Role of the yeast multidrug transporter Qdr2 in cation homeostasis and the oxidative stress response
We have identified QDR2 in a screening for genes able to confer tolerance to sodium and/or lithium stress upon overexpression. Qdr2 is a multidrug transporter of the major facilitator superfamily, originally described for its ability to transport the antimalarial drug quinidine and the herbicide bar...
| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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Oxford University Press
2020
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/6495 https://academic.oup.com/femsyr/article/13/1/97/545699 |
| _version_ | 1855032437421113344 |
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| author | Ríos, Gabino Cabedo-López, Marc Rull, Baltasar Yenush, Lynne Serrano, Ramón Mulet, José M. |
| author_browse | Cabedo-López, Marc Mulet, José M. Rull, Baltasar Ríos, Gabino Serrano, Ramón Yenush, Lynne |
| author_facet | Ríos, Gabino Cabedo-López, Marc Rull, Baltasar Yenush, Lynne Serrano, Ramón Mulet, José M. |
| author_sort | Ríos, Gabino |
| collection | ReDivia |
| description | We have identified QDR2 in a screening for genes able to confer tolerance to sodium and/or lithium stress upon overexpression. Qdr2 is a multidrug transporter of the major facilitator superfamily, originally described for its ability to transport the antimalarial drug quinidine and the herbicide barban. To identify its physiological substrate, we have screened for phenotypes dependent on QDR2 and found that Qdr2 is able to transport monovalent and divalent cations with poor selectivity, as shown by growth tests and the determination of internal cation content. Moreover, strains overexpressing or lacking QDR2 also exhibit phenotypes when reactive oxygen species- producing agents, such as hydrogen peroxide or menadione were added to the growth medium. We have also found that the presence of copper and hydrogen peroxide repress the expression of QDR2. In addition, the copper uptake of a qdr2 mutant strain is similar to a wild type, but the extrusion is clearly impaired. Based on our results, we propose that free divalent copper is the main physiological substrate of Qdr2. As copper is a substrate for several redox reactions that occur within the cytoplasm, its function in copper homeostasis explains its role in the oxidative stress response. |
| format | article |
| id | ReDivia6495 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | ReDivia64952025-04-25T14:47:17Z Role of the yeast multidrug transporter Qdr2 in cation homeostasis and the oxidative stress response Ríos, Gabino Cabedo-López, Marc Rull, Baltasar Yenush, Lynne Serrano, Ramón Mulet, José M. Ion transport Copper homeostasis F62 Plant physiology - Growth and development Oxidative stress We have identified QDR2 in a screening for genes able to confer tolerance to sodium and/or lithium stress upon overexpression. Qdr2 is a multidrug transporter of the major facilitator superfamily, originally described for its ability to transport the antimalarial drug quinidine and the herbicide barban. To identify its physiological substrate, we have screened for phenotypes dependent on QDR2 and found that Qdr2 is able to transport monovalent and divalent cations with poor selectivity, as shown by growth tests and the determination of internal cation content. Moreover, strains overexpressing or lacking QDR2 also exhibit phenotypes when reactive oxygen species- producing agents, such as hydrogen peroxide or menadione were added to the growth medium. We have also found that the presence of copper and hydrogen peroxide repress the expression of QDR2. In addition, the copper uptake of a qdr2 mutant strain is similar to a wild type, but the extrusion is clearly impaired. Based on our results, we propose that free divalent copper is the main physiological substrate of Qdr2. As copper is a substrate for several redox reactions that occur within the cytoplasm, its function in copper homeostasis explains its role in the oxidative stress response. 2020-06-03T15:40:25Z 2020-06-03T15:40:25Z 2013 article acceptedVersion Ríos, G., Cabedo, M., Rull, B., Yenush, L., Serrano, R., & Mulet, J. M. (2013). Role of the yeast multidrug transporter Qdr2 in cation homeostasis and the oxidative stress response. FEMS yeast research, 13(1), 97-106. 1567-1364 1567-1356 http://hdl.handle.net/20.500.11939/6495 10.1111/1567-1364.12013 https://academic.oup.com/femsyr/article/13/1/97/545699 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Oxford University Press electronico |
| spellingShingle | Ion transport Copper homeostasis F62 Plant physiology - Growth and development Oxidative stress Ríos, Gabino Cabedo-López, Marc Rull, Baltasar Yenush, Lynne Serrano, Ramón Mulet, José M. Role of the yeast multidrug transporter Qdr2 in cation homeostasis and the oxidative stress response |
| title | Role of the yeast multidrug transporter Qdr2 in cation homeostasis and the oxidative stress response |
| title_full | Role of the yeast multidrug transporter Qdr2 in cation homeostasis and the oxidative stress response |
| title_fullStr | Role of the yeast multidrug transporter Qdr2 in cation homeostasis and the oxidative stress response |
| title_full_unstemmed | Role of the yeast multidrug transporter Qdr2 in cation homeostasis and the oxidative stress response |
| title_short | Role of the yeast multidrug transporter Qdr2 in cation homeostasis and the oxidative stress response |
| title_sort | role of the yeast multidrug transporter qdr2 in cation homeostasis and the oxidative stress response |
| topic | Ion transport Copper homeostasis F62 Plant physiology - Growth and development Oxidative stress |
| url | http://hdl.handle.net/20.500.11939/6495 https://academic.oup.com/femsyr/article/13/1/97/545699 |
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