Emergence of a Plant Pathogen in Europe Associated with Multiple Intercontinental Introductions
Pathogen introductions have led to numerous disease outbreaks in naive regions of the globe. The plant pathogen Xylella fastidiosa has been associated with various recent epidemics in Europe affecting agricultural crops, such as almond, grapevine, and olive, but also endemic species occurring in nat...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Applied and Environmental Microbiology
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6491 https://aem.asm.org/content/86/3/e01521-19?rss=1 |
| _version_ | 1855492094542479360 |
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| author | Landa, Blanca B. Castillo, Andreina I. Giampetruzzi, Annalisa kahn, Alexandra Román-Écija, Miguel Velasco-Amo, María P. Navas-Cortés, Juan A. Marco-Noales, Ester Barbé, Silvia Moralejo, Eduardo Coletta-Filho, Helvecio D. Saldarelli, Pasquale Saponari, Maria Almeida, Rodrigo P. P. |
| author_browse | Almeida, Rodrigo P. P. Barbé, Silvia Castillo, Andreina I. Coletta-Filho, Helvecio D. Giampetruzzi, Annalisa Landa, Blanca B. Marco-Noales, Ester Moralejo, Eduardo Navas-Cortés, Juan A. Román-Écija, Miguel Saldarelli, Pasquale Saponari, Maria Velasco-Amo, María P. kahn, Alexandra |
| author_facet | Landa, Blanca B. Castillo, Andreina I. Giampetruzzi, Annalisa kahn, Alexandra Román-Écija, Miguel Velasco-Amo, María P. Navas-Cortés, Juan A. Marco-Noales, Ester Barbé, Silvia Moralejo, Eduardo Coletta-Filho, Helvecio D. Saldarelli, Pasquale Saponari, Maria Almeida, Rodrigo P. P. |
| author_sort | Landa, Blanca B. |
| collection | ReDivia |
| description | Pathogen introductions have led to numerous disease outbreaks in naive regions of the globe. The plant pathogen Xylella fastidiosa has been associated with various recent epidemics in Europe affecting agricultural crops, such as almond, grapevine, and olive, but also endemic species occurring in natural forest landscapes and ornamental plants. We compared whole-genome sequences of X. fastidiosa subspecies multiplex from America and strains associated with recent outbreaks in southern Europe to infer their likely origins and paths of introduction within and between the two continents. Phylogenetic analyses indicated multiple introductions of X. fastidiosa subspecies multiplex into Italy, Spain, and France, most of which emerged from a clade with limited genetic diversity with a likely origin in California, USA. The limited genetic diversity observed in X. fastidiosa subspecies multiplex strains originating from California is likely due to the clade itself being an introduction from X. fastidiosa subspecies multiplex populations in the southeastern United States, where this subspecies is most likely endemic. Despite the genetic diversity found in some areas in Europe, there was no clear evidence of recombination occurring among introduced X. fastidiosa strains in Europe. Sequence type taxonomy, based on multilocus sequence typing (MLST), was shown, at least in one case, to not lead to monophyletic clades of this pathogen; whole-genome sequence data were more informative in resolving the history of introductions than MLST data. Although additional data are necessary to carefully tease out the paths of these recent dispersal events, our results indicate that whole-genome sequence data should be considered when developing management strategies for X. fastidiosa outbreaks.
IMPORTANCE Xylella fastidiosa is an economically important plant-pathogenic bacterium that has emerged as a pathogen of global importance associated with a devastating epidemic in olive trees in Italy associated with X. fastidiosa subspecies pauca and other outbreaks in Europe, such as X. fastidiosa subspecies fastidiosa and X. fastidiosa subspecies multiplex in Spain and X. fastidiosa subspecies multiplex in France. We present evidence of multiple introductions of X. fastidiosa subspecies multiplex, likely from the United States, into Spain, Italy, and France. These introductions illustrate the risks associated with the commercial trade of plant material at global scales and the need to develop effective policy to limit the likelihood of pathogen pollution into naive regions. Our study demonstrates the need to utilize whole-genome sequence data to study X. fastidiosa introductions at outbreak stages, since a limited number of genetic markers does not provide sufficient phylogenetic resolution to determine dispersal paths or relationships among strains that are of biological and quarantine relevance. |
| format | Artículo |
| id | ReDivia6491 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Applied and Environmental Microbiology |
| publisherStr | Applied and Environmental Microbiology |
| record_format | dspace |
| spelling | ReDivia64912025-04-25T14:47:17Z Emergence of a Plant Pathogen in Europe Associated with Multiple Intercontinental Introductions Landa, Blanca B. Castillo, Andreina I. Giampetruzzi, Annalisa kahn, Alexandra Román-Écija, Miguel Velasco-Amo, María P. Navas-Cortés, Juan A. Marco-Noales, Ester Barbé, Silvia Moralejo, Eduardo Coletta-Filho, Helvecio D. Saldarelli, Pasquale Saponari, Maria Almeida, Rodrigo P. P. Emerging disease Genetic diversity Outbreaks H20 Plant diseases Xylella fastidiosa Recombination Quarantine Pathogen introductions have led to numerous disease outbreaks in naive regions of the globe. The plant pathogen Xylella fastidiosa has been associated with various recent epidemics in Europe affecting agricultural crops, such as almond, grapevine, and olive, but also endemic species occurring in natural forest landscapes and ornamental plants. We compared whole-genome sequences of X. fastidiosa subspecies multiplex from America and strains associated with recent outbreaks in southern Europe to infer their likely origins and paths of introduction within and between the two continents. Phylogenetic analyses indicated multiple introductions of X. fastidiosa subspecies multiplex into Italy, Spain, and France, most of which emerged from a clade with limited genetic diversity with a likely origin in California, USA. The limited genetic diversity observed in X. fastidiosa subspecies multiplex strains originating from California is likely due to the clade itself being an introduction from X. fastidiosa subspecies multiplex populations in the southeastern United States, where this subspecies is most likely endemic. Despite the genetic diversity found in some areas in Europe, there was no clear evidence of recombination occurring among introduced X. fastidiosa strains in Europe. Sequence type taxonomy, based on multilocus sequence typing (MLST), was shown, at least in one case, to not lead to monophyletic clades of this pathogen; whole-genome sequence data were more informative in resolving the history of introductions than MLST data. Although additional data are necessary to carefully tease out the paths of these recent dispersal events, our results indicate that whole-genome sequence data should be considered when developing management strategies for X. fastidiosa outbreaks. IMPORTANCE Xylella fastidiosa is an economically important plant-pathogenic bacterium that has emerged as a pathogen of global importance associated with a devastating epidemic in olive trees in Italy associated with X. fastidiosa subspecies pauca and other outbreaks in Europe, such as X. fastidiosa subspecies fastidiosa and X. fastidiosa subspecies multiplex in Spain and X. fastidiosa subspecies multiplex in France. We present evidence of multiple introductions of X. fastidiosa subspecies multiplex, likely from the United States, into Spain, Italy, and France. These introductions illustrate the risks associated with the commercial trade of plant material at global scales and the need to develop effective policy to limit the likelihood of pathogen pollution into naive regions. Our study demonstrates the need to utilize whole-genome sequence data to study X. fastidiosa introductions at outbreak stages, since a limited number of genetic markers does not provide sufficient phylogenetic resolution to determine dispersal paths or relationships among strains that are of biological and quarantine relevance. 2020-06-02T12:51:46Z 2020-06-02T12:51:46Z 2020 article acceptedVersion Landa, B. B., Castillo, A. I., Giampetruzzi, A., Kahn, A., Román-Écija, M., Velasco-Amo, M. P., ... & Coletta-Filho, H. D. (2020). Emergence of a plant pathogen in Europe associated with multiple intercontinental introductions. Applied and Environmental Microbiology, 86(3). 0099-2240 1098-5336 http://hdl.handle.net/20.500.11939/6491 10.1128/AEM.01521-19 https://aem.asm.org/content/86/3/e01521-19?rss=1 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Applied and Environmental Microbiology electronico |
| spellingShingle | Emerging disease Genetic diversity Outbreaks H20 Plant diseases Xylella fastidiosa Recombination Quarantine Landa, Blanca B. Castillo, Andreina I. Giampetruzzi, Annalisa kahn, Alexandra Román-Écija, Miguel Velasco-Amo, María P. Navas-Cortés, Juan A. Marco-Noales, Ester Barbé, Silvia Moralejo, Eduardo Coletta-Filho, Helvecio D. Saldarelli, Pasquale Saponari, Maria Almeida, Rodrigo P. P. Emergence of a Plant Pathogen in Europe Associated with Multiple Intercontinental Introductions |
| title | Emergence of a Plant Pathogen in Europe Associated with Multiple Intercontinental Introductions |
| title_full | Emergence of a Plant Pathogen in Europe Associated with Multiple Intercontinental Introductions |
| title_fullStr | Emergence of a Plant Pathogen in Europe Associated with Multiple Intercontinental Introductions |
| title_full_unstemmed | Emergence of a Plant Pathogen in Europe Associated with Multiple Intercontinental Introductions |
| title_short | Emergence of a Plant Pathogen in Europe Associated with Multiple Intercontinental Introductions |
| title_sort | emergence of a plant pathogen in europe associated with multiple intercontinental introductions |
| topic | Emerging disease Genetic diversity Outbreaks H20 Plant diseases Xylella fastidiosa Recombination Quarantine |
| url | http://hdl.handle.net/20.500.11939/6491 https://aem.asm.org/content/86/3/e01521-19?rss=1 |
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