MBW complexes impinge on anthocyanidin reductase gene regulation for proanthocyanidin biosynthesis in persimmon fruit
MBW protein complexes containing MYB, bHLH and WD40 repeat factors are known transcriptional regulators of secondary metabolites production such as proanthocyanidins and anthocyanins, and developmental processes such as trichome formation in many plant species. DkMYB2 and DkMYB4 (MYB-type), DkMYC1 (...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
Springer Nature
2020
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6490 https://www.nature.com/articles/s41598-020-60635-w |
| _version_ | 1855032436552892416 |
|---|---|
| author | Gil-Muñoz, Francisco Sánchez-Navarro, Jesús A. Besada, Cristina Salvador, Alejandra Badenes, María L. Naval, María M. Ríos, Gabino |
| author_browse | Badenes, María L. Besada, Cristina Gil-Muñoz, Francisco Naval, María M. Ríos, Gabino Salvador, Alejandra Sánchez-Navarro, Jesús A. |
| author_facet | Gil-Muñoz, Francisco Sánchez-Navarro, Jesús A. Besada, Cristina Salvador, Alejandra Badenes, María L. Naval, María M. Ríos, Gabino |
| author_sort | Gil-Muñoz, Francisco |
| collection | ReDivia |
| description | MBW protein complexes containing MYB, bHLH and WD40 repeat factors are known transcriptional regulators of secondary metabolites production such as proanthocyanidins and anthocyanins, and developmental processes such as trichome formation in many plant species. DkMYB2 and DkMYB4 (MYB-type), DkMYC1 (bHLH-type) and DkWDR1 (WD40-type) factors have been proposed by different authors to take part of persimmon MBW complexes for proanthocyanidin accumulation in immature fruit, leading to its characteristic astringent flavour with important agronomical and ecological effects. We have confirmed the nuclear localization of these proteins and their mutual physical interaction by bimolecular fluorescence complementation analysis. In addition, transient expression of DkMYB2, DkMYB4 and DkMYC1 cooperatively increase the expression of a persimmon anthocyanidin reductase gene (ANR), involved in the biosynthesis of cis-flavan-3-ols, the structural units of proanthocyanidin compounds. Collectively, these data support the presence of MBW complexes in persimmon fruit and suggest their coordinated participation in ANR regulation for proanthocyanidin production. |
| format | article |
| id | ReDivia6490 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | dspace |
| spelling | ReDivia64902025-04-25T14:47:16Z MBW complexes impinge on anthocyanidin reductase gene regulation for proanthocyanidin biosynthesis in persimmon fruit Gil-Muñoz, Francisco Sánchez-Navarro, Jesús A. Besada, Cristina Salvador, Alejandra Badenes, María L. Naval, María M. Ríos, Gabino Agricultural genetics Plant development Secondary metabolism F30 Plant genetics and breeding Fruiting Plant breeding MBW protein complexes containing MYB, bHLH and WD40 repeat factors are known transcriptional regulators of secondary metabolites production such as proanthocyanidins and anthocyanins, and developmental processes such as trichome formation in many plant species. DkMYB2 and DkMYB4 (MYB-type), DkMYC1 (bHLH-type) and DkWDR1 (WD40-type) factors have been proposed by different authors to take part of persimmon MBW complexes for proanthocyanidin accumulation in immature fruit, leading to its characteristic astringent flavour with important agronomical and ecological effects. We have confirmed the nuclear localization of these proteins and their mutual physical interaction by bimolecular fluorescence complementation analysis. In addition, transient expression of DkMYB2, DkMYB4 and DkMYC1 cooperatively increase the expression of a persimmon anthocyanidin reductase gene (ANR), involved in the biosynthesis of cis-flavan-3-ols, the structural units of proanthocyanidin compounds. Collectively, these data support the presence of MBW complexes in persimmon fruit and suggest their coordinated participation in ANR regulation for proanthocyanidin production. 2020-06-02T08:20:30Z 2020-06-02T08:20:30Z 2020 article publishedVersion Gil-Muñoz, F., Sánchez-Navarro, J. A., Besada, C., Salvador, A., Badenes, M. L., Naval, M. M. et al. (2020). MBW complexes impinge on anthocyanidin reductase gene regulation for proanthocyanidin biosynthesis in persimmon fruit. Scientific reports, 10(1), 1-11. 2045-2322 http://hdl.handle.net/20.500.11939/6490 10.1038/s41598-020-60635-w https://www.nature.com/articles/s41598-020-60635-w en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Springer Nature electronico |
| spellingShingle | Agricultural genetics Plant development Secondary metabolism F30 Plant genetics and breeding Fruiting Plant breeding Gil-Muñoz, Francisco Sánchez-Navarro, Jesús A. Besada, Cristina Salvador, Alejandra Badenes, María L. Naval, María M. Ríos, Gabino MBW complexes impinge on anthocyanidin reductase gene regulation for proanthocyanidin biosynthesis in persimmon fruit |
| title | MBW complexes impinge on anthocyanidin reductase gene regulation for proanthocyanidin biosynthesis in persimmon fruit |
| title_full | MBW complexes impinge on anthocyanidin reductase gene regulation for proanthocyanidin biosynthesis in persimmon fruit |
| title_fullStr | MBW complexes impinge on anthocyanidin reductase gene regulation for proanthocyanidin biosynthesis in persimmon fruit |
| title_full_unstemmed | MBW complexes impinge on anthocyanidin reductase gene regulation for proanthocyanidin biosynthesis in persimmon fruit |
| title_short | MBW complexes impinge on anthocyanidin reductase gene regulation for proanthocyanidin biosynthesis in persimmon fruit |
| title_sort | mbw complexes impinge on anthocyanidin reductase gene regulation for proanthocyanidin biosynthesis in persimmon fruit |
| topic | Agricultural genetics Plant development Secondary metabolism F30 Plant genetics and breeding Fruiting Plant breeding |
| url | http://hdl.handle.net/20.500.11939/6490 https://www.nature.com/articles/s41598-020-60635-w |
| work_keys_str_mv | AT gilmunozfrancisco mbwcomplexesimpingeonanthocyanidinreductasegeneregulationforproanthocyanidinbiosynthesisinpersimmonfruit AT sancheznavarrojesusa mbwcomplexesimpingeonanthocyanidinreductasegeneregulationforproanthocyanidinbiosynthesisinpersimmonfruit AT besadacristina mbwcomplexesimpingeonanthocyanidinreductasegeneregulationforproanthocyanidinbiosynthesisinpersimmonfruit AT salvadoralejandra mbwcomplexesimpingeonanthocyanidinreductasegeneregulationforproanthocyanidinbiosynthesisinpersimmonfruit AT badenesmarial mbwcomplexesimpingeonanthocyanidinreductasegeneregulationforproanthocyanidinbiosynthesisinpersimmonfruit AT navalmariam mbwcomplexesimpingeonanthocyanidinreductasegeneregulationforproanthocyanidinbiosynthesisinpersimmonfruit AT riosgabino mbwcomplexesimpingeonanthocyanidinreductasegeneregulationforproanthocyanidinbiosynthesisinpersimmonfruit |