Genetic Analysis of Tomato Root Colonization by Arbuscular Mycorrhizal Fungi
Background and Aims Arbuscular mycorrhizal fungi (AMF) play an important role in plant nutrition and protection against pests and diseases, as well as in soil structuration, nutrient cycling and, generally speaking, in sustainable agriculture, particularly under drought, salinity and low input or o...
| Main Authors: | , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Oxford University Press
2020
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| Online Access: | http://hdl.handle.net/20.500.11939/6487 https://academic.oup.com/aob/article/124/6/933/5312893 |
| _version_ | 1855492093709910016 |
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| author | Plouznikoff, Katia Asins, María J. Dupré de Boulois, Hervé Carbonell, Emilio A. Declerck, Stéphane |
| author_browse | Asins, María J. Carbonell, Emilio A. Declerck, Stéphane Dupré de Boulois, Hervé Plouznikoff, Katia |
| author_facet | Plouznikoff, Katia Asins, María J. Dupré de Boulois, Hervé Carbonell, Emilio A. Declerck, Stéphane |
| author_sort | Plouznikoff, Katia |
| collection | ReDivia |
| description | Background and Aims
Arbuscular mycorrhizal fungi (AMF) play an important role in plant nutrition and protection against pests and diseases, as well as in soil structuration, nutrient cycling and, generally speaking, in sustainable agriculture, particularly under drought, salinity and low input or organic agriculture. However, little is known about the genetics of the AMF–plant association in tomato. The aim of this study was the genetic analysis of root AMF colonization in tomato via the detection of the quantitative trait loci (QTLs) involved.
Methods
A population of 130 recombinant inbred lines derived from the wild species Solanum pimpinellifolium, genotyped for 1899 segregating, non-redundant single nucleotide polymorphisms (SNPs) from the SolCAP tomato panel, was characterized for intensity, frequency and arbuscular abundance of AMF colonization to detect the QTLs involved and to analyse the genes within their peaks (2–2.6 Mbp).
Key Results
The three AMF colonization parameters were highly correlated (0.78–0.97) and the best one, with the highest heritability (0.23), corresponded to colonization intensity. A total of eight QTLs in chromosomes 1, 3, 4, 5, 6, 8, 9 and 10 were detected. Seven of them simultaneously affected intensity and arbuscule abundance. The allele increasing the expression of the trait usually came from the wild parent in accordance with the parental means, and several epistatic interactions were found relevant for breeding purposes. SlCCaMK and SlLYK13 were found among the candidate genes. Carbohydrate transmembrane transporter activity, lipid metabolism and transport, metabolic processes related to nitrogen and phosphate-containing compounds, regulation of carbohydrates, and other biological processes involved in the plant defence were found to be over-represented within the QTL peaks.
Conclusions
Intensity is genetically the best morphological measure of tomato root AMF colonization. Wild alleles can improve AMF colonization, and the gene contents of AMF colonization QTLs might be important for explaining the establishment and functioning of the AMF–plant symbiosis. |
| format | Artículo |
| id | ReDivia6487 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | ReDivia64872025-04-25T14:47:15Z Genetic Analysis of Tomato Root Colonization by Arbuscular Mycorrhizal Fungi Plouznikoff, Katia Asins, María J. Dupré de Boulois, Hervé Carbonell, Emilio A. Declerck, Stéphane AMF colonization QTL analysis Candidate genes solCAP SNPs Solanum pimpinellifolium Biological process Underdominance F30 Plant genetics and breeding Heritability Solanum lycopersicum Epistasis Background and Aims Arbuscular mycorrhizal fungi (AMF) play an important role in plant nutrition and protection against pests and diseases, as well as in soil structuration, nutrient cycling and, generally speaking, in sustainable agriculture, particularly under drought, salinity and low input or organic agriculture. However, little is known about the genetics of the AMF–plant association in tomato. The aim of this study was the genetic analysis of root AMF colonization in tomato via the detection of the quantitative trait loci (QTLs) involved. Methods A population of 130 recombinant inbred lines derived from the wild species Solanum pimpinellifolium, genotyped for 1899 segregating, non-redundant single nucleotide polymorphisms (SNPs) from the SolCAP tomato panel, was characterized for intensity, frequency and arbuscular abundance of AMF colonization to detect the QTLs involved and to analyse the genes within their peaks (2–2.6 Mbp). Key Results The three AMF colonization parameters were highly correlated (0.78–0.97) and the best one, with the highest heritability (0.23), corresponded to colonization intensity. A total of eight QTLs in chromosomes 1, 3, 4, 5, 6, 8, 9 and 10 were detected. Seven of them simultaneously affected intensity and arbuscule abundance. The allele increasing the expression of the trait usually came from the wild parent in accordance with the parental means, and several epistatic interactions were found relevant for breeding purposes. SlCCaMK and SlLYK13 were found among the candidate genes. Carbohydrate transmembrane transporter activity, lipid metabolism and transport, metabolic processes related to nitrogen and phosphate-containing compounds, regulation of carbohydrates, and other biological processes involved in the plant defence were found to be over-represented within the QTL peaks. Conclusions Intensity is genetically the best morphological measure of tomato root AMF colonization. Wild alleles can improve AMF colonization, and the gene contents of AMF colonization QTLs might be important for explaining the establishment and functioning of the AMF–plant symbiosis. 2020-06-02T08:16:05Z 2020-06-02T08:16:05Z 2019 article Plouznikoff, K., Asins, M. J., de Boulois, H. D., Carbonell, E. A., & Declerck, S. (2019). Genetic analysis of tomato root colonization by arbuscular mycorrhizal fungi. Annals of botany, 124(6), 933-946. 1095-8290 0305-7364 http://hdl.handle.net/20.500.11939/6487 10.1093/aob/mcy240 https://academic.oup.com/aob/article/124/6/933/5312893 en closedAccess Oxford University Press electronico |
| spellingShingle | AMF colonization QTL analysis Candidate genes solCAP SNPs Solanum pimpinellifolium Biological process Underdominance F30 Plant genetics and breeding Heritability Solanum lycopersicum Epistasis Plouznikoff, Katia Asins, María J. Dupré de Boulois, Hervé Carbonell, Emilio A. Declerck, Stéphane Genetic Analysis of Tomato Root Colonization by Arbuscular Mycorrhizal Fungi |
| title | Genetic Analysis of Tomato Root Colonization by Arbuscular Mycorrhizal Fungi |
| title_full | Genetic Analysis of Tomato Root Colonization by Arbuscular Mycorrhizal Fungi |
| title_fullStr | Genetic Analysis of Tomato Root Colonization by Arbuscular Mycorrhizal Fungi |
| title_full_unstemmed | Genetic Analysis of Tomato Root Colonization by Arbuscular Mycorrhizal Fungi |
| title_short | Genetic Analysis of Tomato Root Colonization by Arbuscular Mycorrhizal Fungi |
| title_sort | genetic analysis of tomato root colonization by arbuscular mycorrhizal fungi |
| topic | AMF colonization QTL analysis Candidate genes solCAP SNPs Solanum pimpinellifolium Biological process Underdominance F30 Plant genetics and breeding Heritability Solanum lycopersicum Epistasis |
| url | http://hdl.handle.net/20.500.11939/6487 https://academic.oup.com/aob/article/124/6/933/5312893 |
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