The G123 rice mutant, carrying a mutation in SE13, presents alterations in the expression patterns of photosynthetic and major flowering regulatory genes
Day length is a determinant of flowering time in rice. Phytochromes participate in flowering regulation by measuring the number of daylight hours to which the plant is exposed. Here we describe G123, a rice mutant generated by irradiation, which displays insensitivity to the photoperiod and early fl...
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| Format: | publishedVersion |
| Language: | Inglés |
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Plos
2020
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| Online Access: | http://hdl.handle.net/20.500.11939/6452 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0233120 |
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| author | Reig-Valiente, Juan L. Borredá, Carles Talón, Manuel Domingo, Concha |
| author_browse | Borredá, Carles Domingo, Concha Reig-Valiente, Juan L. Talón, Manuel |
| author_facet | Reig-Valiente, Juan L. Borredá, Carles Talón, Manuel Domingo, Concha |
| author_sort | Reig-Valiente, Juan L. |
| collection | ReDivia |
| description | Day length is a determinant of flowering time in rice. Phytochromes participate in flowering regulation by measuring the number of daylight hours to which the plant is exposed. Here we describe G123, a rice mutant generated by irradiation, which displays insensitivity to the photoperiod and early flowering under both long day and short day conditions. To detect the mutation responsible for the early flowering phenotype exhibited by G123, we generated an F2 population, derived from crossing with the wild-type, and used a pipeline to detect genomic structural variation, initially developed for human genomes. We detected a deletion in the G123 genome that affects the PHOTOPERIOD SENSITIVITY13 (SE13) gene, which encodes a phytochromobilin synthase, an enzyme implicated in phytochrome chromophore biosynthesis. The transcriptomic analysis, performed by RNA-seq, in the G123 plants indicated an alteration in photosynthesis and other processes related to response to light. The expression patterns of the main flowering regulatory genes, such as Ghd7, Ghd8 and PRR37, were altered in the plants grown under both long day and short day conditions. These findings indicate that phytochromes are also involved in the regulation of these genes under short day conditions, and extend the role of phytochromes in flowering regulation in rice |
| format | publishedVersion |
| id | ReDivia6452 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Plos |
| publisherStr | Plos |
| record_format | dspace |
| spelling | ReDivia64522025-04-25T14:47:08Z The G123 rice mutant, carrying a mutation in SE13, presents alterations in the expression patterns of photosynthetic and major flowering regulatory genes Reig-Valiente, Juan L. Borredá, Carles Talón, Manuel Domingo, Concha F60 Plant physiology and biochemistry Rice Flowering Day length is a determinant of flowering time in rice. Phytochromes participate in flowering regulation by measuring the number of daylight hours to which the plant is exposed. Here we describe G123, a rice mutant generated by irradiation, which displays insensitivity to the photoperiod and early flowering under both long day and short day conditions. To detect the mutation responsible for the early flowering phenotype exhibited by G123, we generated an F2 population, derived from crossing with the wild-type, and used a pipeline to detect genomic structural variation, initially developed for human genomes. We detected a deletion in the G123 genome that affects the PHOTOPERIOD SENSITIVITY13 (SE13) gene, which encodes a phytochromobilin synthase, an enzyme implicated in phytochrome chromophore biosynthesis. The transcriptomic analysis, performed by RNA-seq, in the G123 plants indicated an alteration in photosynthesis and other processes related to response to light. The expression patterns of the main flowering regulatory genes, such as Ghd7, Ghd8 and PRR37, were altered in the plants grown under both long day and short day conditions. These findings indicate that phytochromes are also involved in the regulation of these genes under short day conditions, and extend the role of phytochromes in flowering regulation in rice 2020-05-21T09:34:31Z 2020-05-21T09:34:31Z 2020 publishedVersion Reig-Valiente, J. L, Borredá, C., Talón, M. & Domingo C. (2020). The G123 rice mutant, carrying a mutation in SE13, presents alterations in the expression patterns of photosynthetic and major flowering regulatory genes. PLoS ONE 15(5): e0233120. http://hdl.handle.net/20.500.11939/6452 10.1371/journal.pone.0233120 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0233120 en_US info:eu-repo/grantAgreement/MINECO/Programa estatal de i+D+i Orientada a los Retos de la Sociedad/RTA2014-00058-C03-01//Generación de recursos genéticos y de conocimiento para la obtención de nuevas variedades de arroz portadoras de mejoras en los caracteres más relevantes del cultivo en todas las zonas arroceras de España RTA2014-00058-C03-01 01/14-FSE-0 ACIF/2016/117 Plos electronico |
| spellingShingle | F60 Plant physiology and biochemistry Rice Flowering Reig-Valiente, Juan L. Borredá, Carles Talón, Manuel Domingo, Concha The G123 rice mutant, carrying a mutation in SE13, presents alterations in the expression patterns of photosynthetic and major flowering regulatory genes |
| title | The G123 rice mutant, carrying a mutation in SE13, presents alterations in the expression patterns of photosynthetic and major flowering regulatory genes |
| title_full | The G123 rice mutant, carrying a mutation in SE13, presents alterations in the expression patterns of photosynthetic and major flowering regulatory genes |
| title_fullStr | The G123 rice mutant, carrying a mutation in SE13, presents alterations in the expression patterns of photosynthetic and major flowering regulatory genes |
| title_full_unstemmed | The G123 rice mutant, carrying a mutation in SE13, presents alterations in the expression patterns of photosynthetic and major flowering regulatory genes |
| title_short | The G123 rice mutant, carrying a mutation in SE13, presents alterations in the expression patterns of photosynthetic and major flowering regulatory genes |
| title_sort | g123 rice mutant carrying a mutation in se13 presents alterations in the expression patterns of photosynthetic and major flowering regulatory genes |
| topic | F60 Plant physiology and biochemistry Rice Flowering |
| url | http://hdl.handle.net/20.500.11939/6452 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0233120 |
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