Non-destructive assessment of chloride in persimmon leaves using a miniature visible near-infrared spectrometer
New methods for the non-destructive, rapid and reliable assessment of leaf chloride (Cl−) are needed in many agricultural salt-threatened areas throughout the World. Although portable visible near-infrared spectrometers could be useful for this task, accompanying models for leaf Cl− assessment on...
| Main Authors: | , |
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| Format: | acceptedVersion |
| Language: | Inglés |
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Elsevier
2020
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| Online Access: | http://hdl.handle.net/20.500.11939/6451 https://www.sciencedirect.com/science/article/pii/S0168169919302212?via%3Dihub |
| _version_ | 1855032429278920704 |
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| author | Visconti, Fernando De-Paz, José M. |
| author_browse | De-Paz, José M. Visconti, Fernando |
| author_facet | Visconti, Fernando De-Paz, José M. |
| author_sort | Visconti, Fernando |
| collection | ReDivia |
| description | New methods for the non-destructive, rapid and reliable assessment of leaf chloride (Cl−) are needed in many
agricultural salt-threatened areas throughout the World. Although portable visible near-infrared spectrometers
could be useful for this task, accompanying models for leaf Cl− assessment on basis their visible near-infrared
(Vis-NIR) spectra had yet to be developed. To study the feasibility of such models, a persimmon orchard was
grown in E Spain and irrigated with eight waters ranging in Cl− concentration from 70 to 350 mg/L. Then, six
times throughout the growing season the Vis-NIR foliar reflectance spectra of various representative leaves per
Cl− treatment were recorded with a portable miniature Vis-NIR spectrometer. The leaf Cl− was subsequently
determined in the same leaves with a laboratory reference method. Next, both the Cl− and spectral data were
analyzed using descriptive statistics, mathematical data transformations, principal components analysis (PCA)
and partial least squares regression (PLSR). A wide, sufficiently normal-distributed and representative range of
leaf Cl− content from 0.17 to 3.14%Cl− was obtained. With correlation coefficients exceeding +0.65 between
390 and 472 nm and between 690 and 692 nm, the foliar reflectance was the spectral data correlated the most
with the leaf Cl−. Remarkably, both wavelengths intervals are where chlorophyll a has its two absorbance
maxima in the visible region. A PLSR model including all the wavelengths in the Vis-NIR reflectance spectra plus
the day of the year, attained values of R2=0.78, RMSE=0.34%Cl− and RPD=2.18 in the external validation.
However, for leaf Cl− below 1.5%Cl−, i.e., when Cl− assessment is most needed, this model presented
R2=0.55, but RMSE=0.08%Cl− and RPD=5.5, in addition to a bias of +0.04%Cl− and a standard error of
0.05%Cl−. These results show that portable Vis-NIR spectrometers can be useful for the non-destructive, rapid
and even reliable assessment of leaf Cl−. The potential of this methodology for other crop plants and inorganic
ions should be tested. |
| format | acceptedVersion |
| id | ReDivia6451 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | ReDivia64512025-04-25T14:47:08Z Non-destructive assessment of chloride in persimmon leaves using a miniature visible near-infrared spectrometer Visconti, Fernando De-Paz, José M. Chemometrics Visible-NIR spectroscopy Persimmon Partial least squares N20 Agricultural machinery and equipment Chlorides New methods for the non-destructive, rapid and reliable assessment of leaf chloride (Cl−) are needed in many agricultural salt-threatened areas throughout the World. Although portable visible near-infrared spectrometers could be useful for this task, accompanying models for leaf Cl− assessment on basis their visible near-infrared (Vis-NIR) spectra had yet to be developed. To study the feasibility of such models, a persimmon orchard was grown in E Spain and irrigated with eight waters ranging in Cl− concentration from 70 to 350 mg/L. Then, six times throughout the growing season the Vis-NIR foliar reflectance spectra of various representative leaves per Cl− treatment were recorded with a portable miniature Vis-NIR spectrometer. The leaf Cl− was subsequently determined in the same leaves with a laboratory reference method. Next, both the Cl− and spectral data were analyzed using descriptive statistics, mathematical data transformations, principal components analysis (PCA) and partial least squares regression (PLSR). A wide, sufficiently normal-distributed and representative range of leaf Cl− content from 0.17 to 3.14%Cl− was obtained. With correlation coefficients exceeding +0.65 between 390 and 472 nm and between 690 and 692 nm, the foliar reflectance was the spectral data correlated the most with the leaf Cl−. Remarkably, both wavelengths intervals are where chlorophyll a has its two absorbance maxima in the visible region. A PLSR model including all the wavelengths in the Vis-NIR reflectance spectra plus the day of the year, attained values of R2=0.78, RMSE=0.34%Cl− and RPD=2.18 in the external validation. However, for leaf Cl− below 1.5%Cl−, i.e., when Cl− assessment is most needed, this model presented R2=0.55, but RMSE=0.08%Cl− and RPD=5.5, in addition to a bias of +0.04%Cl− and a standard error of 0.05%Cl−. These results show that portable Vis-NIR spectrometers can be useful for the non-destructive, rapid and even reliable assessment of leaf Cl−. The potential of this methodology for other crop plants and inorganic ions should be tested. 2020-05-19T17:20:37Z 2020-05-19T17:20:37Z 2019 acceptedVersion Visconti, F., & de Paz, J. M. (2019). Non-destructive assessment of chloride in persimmon leaves using a miniature visible near-infrared spectrometer. Computers and Electronics in Agriculture, 164, 104894. 0168-1699 http://hdl.handle.net/20.500.11939/6451 10.1016/j.compag.2019.104894 https://www.sciencedirect.com/science/article/pii/S0168169919302212?via%3Dihub en Atribución-NoComercial-SinDerivadas 3.0 España Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Elsevier electronico |
| spellingShingle | Chemometrics Visible-NIR spectroscopy Persimmon Partial least squares N20 Agricultural machinery and equipment Chlorides Visconti, Fernando De-Paz, José M. Non-destructive assessment of chloride in persimmon leaves using a miniature visible near-infrared spectrometer |
| title | Non-destructive assessment of chloride in persimmon leaves using a miniature visible near-infrared spectrometer |
| title_full | Non-destructive assessment of chloride in persimmon leaves using a miniature visible near-infrared spectrometer |
| title_fullStr | Non-destructive assessment of chloride in persimmon leaves using a miniature visible near-infrared spectrometer |
| title_full_unstemmed | Non-destructive assessment of chloride in persimmon leaves using a miniature visible near-infrared spectrometer |
| title_short | Non-destructive assessment of chloride in persimmon leaves using a miniature visible near-infrared spectrometer |
| title_sort | non destructive assessment of chloride in persimmon leaves using a miniature visible near infrared spectrometer |
| topic | Chemometrics Visible-NIR spectroscopy Persimmon Partial least squares N20 Agricultural machinery and equipment Chlorides |
| url | http://hdl.handle.net/20.500.11939/6451 https://www.sciencedirect.com/science/article/pii/S0168169919302212?via%3Dihub |
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