Genetic diversity reflects geographical origin of Ralstonia solanacearum strains isolated from plant and water sources in Spain
The characterization and intraspecific diversity of a collection of 45 Ralstonia solanacearum strains isolated in Spain from different sources and geographical origins is reported. To test the influence of the site and the host on strain diversity, phenotypic and genotypic analysis were performed...
| Autores principales: | , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
Sociedad Española de Microbiología
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6450 https://link.springer.com/journal/10123/volumes-and-issues |
| _version_ | 1855032429095419904 |
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| author | Caruso, Paola Biosca, Elena G. Bertolini, Edson Marco-Noales, Ester Gorris, María T. Licciardello, Concetta López, María M. |
| author_browse | Bertolini, Edson Biosca, Elena G. Caruso, Paola Gorris, María T. Licciardello, Concetta López, María M. Marco-Noales, Ester |
| author_facet | Caruso, Paola Biosca, Elena G. Bertolini, Edson Marco-Noales, Ester Gorris, María T. Licciardello, Concetta López, María M. |
| author_sort | Caruso, Paola |
| collection | ReDivia |
| description | The characterization and intraspecific diversity of a collection of 45 Ralstonia solanacearum strains isolated
in Spain from different sources and geographical origins is reported. To test the influence of the site and the host on strain
diversity, phenotypic and genotypic analysis were performed by a polyphasic approach. Biochemical and metabolic profiles
were compared. Serological relationship was evaluated by Indirect-ELISA using polyclonal and monoclonal antibodies.
For genotypic analysis, hrpB and egl DNA sequence analysis, repetitive sequences (rep-PCR), amplified fragment length
polymorphism (AFLP) profiles and macrorestriction with XbaI followed by pulsed field gel electrophoresis (PFGE) were
performed.
The biochemical and metabolic characterization, serological tests, rep-PCR typing and phylogenetic analysis showed that all
analysed strains belonged to phylotype II sequevar 1 and shared homogeneous profiles. However, interesting differences among
strains were found by AFLP and macrorestriction with XbaI followed by PFGE techniques, some profiles being related to the
geographical origin of the strains. Diversity results obtained offer new insights into the biogeography of this quarantine organism
and its possible sources and reservoirs in Spain and Mediterranean countries. |
| format | article |
| id | ReDivia6450 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Sociedad Española de Microbiología |
| publisherStr | Sociedad Española de Microbiología |
| record_format | dspace |
| spelling | ReDivia64502025-04-25T14:47:08Z Genetic diversity reflects geographical origin of Ralstonia solanacearum strains isolated from plant and water sources in Spain Caruso, Paola Biosca, Elena G. Bertolini, Edson Marco-Noales, Ester Gorris, María T. Licciardello, Concetta López, María M. Bacterial wilt PFGE AFLP F30 Plant genetics and breeding Potatoes Soil The characterization and intraspecific diversity of a collection of 45 Ralstonia solanacearum strains isolated in Spain from different sources and geographical origins is reported. To test the influence of the site and the host on strain diversity, phenotypic and genotypic analysis were performed by a polyphasic approach. Biochemical and metabolic profiles were compared. Serological relationship was evaluated by Indirect-ELISA using polyclonal and monoclonal antibodies. For genotypic analysis, hrpB and egl DNA sequence analysis, repetitive sequences (rep-PCR), amplified fragment length polymorphism (AFLP) profiles and macrorestriction with XbaI followed by pulsed field gel electrophoresis (PFGE) were performed. The biochemical and metabolic characterization, serological tests, rep-PCR typing and phylogenetic analysis showed that all analysed strains belonged to phylotype II sequevar 1 and shared homogeneous profiles. However, interesting differences among strains were found by AFLP and macrorestriction with XbaI followed by PFGE techniques, some profiles being related to the geographical origin of the strains. Diversity results obtained offer new insights into the biogeography of this quarantine organism and its possible sources and reservoirs in Spain and Mediterranean countries. 2020-05-19T16:43:06Z 2020-05-19T16:43:06Z 2017 article publishedVersion Caruso, P., Biosca, E. G., Bertolini, E., Marco-Noales, E., Gorris, M. T., Licciardello, C., & López, M. M. (2017). Genetic diversity reflects geographical origin of Ralstonia solanacearum strains isolated from plant and water sources in Spain. International Microbiology, 20(4), 155-164. 1139-6709 http://hdl.handle.net/20.500.11939/6450 10.2436/20.1501.01.298 https://link.springer.com/journal/10123/volumes-and-issues en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Sociedad Española de Microbiología electronico |
| spellingShingle | Bacterial wilt PFGE AFLP F30 Plant genetics and breeding Potatoes Soil Caruso, Paola Biosca, Elena G. Bertolini, Edson Marco-Noales, Ester Gorris, María T. Licciardello, Concetta López, María M. Genetic diversity reflects geographical origin of Ralstonia solanacearum strains isolated from plant and water sources in Spain |
| title | Genetic diversity reflects geographical origin of Ralstonia solanacearum strains isolated from plant and water sources in Spain |
| title_full | Genetic diversity reflects geographical origin of Ralstonia solanacearum strains isolated from plant and water sources in Spain |
| title_fullStr | Genetic diversity reflects geographical origin of Ralstonia solanacearum strains isolated from plant and water sources in Spain |
| title_full_unstemmed | Genetic diversity reflects geographical origin of Ralstonia solanacearum strains isolated from plant and water sources in Spain |
| title_short | Genetic diversity reflects geographical origin of Ralstonia solanacearum strains isolated from plant and water sources in Spain |
| title_sort | genetic diversity reflects geographical origin of ralstonia solanacearum strains isolated from plant and water sources in spain |
| topic | Bacterial wilt PFGE AFLP F30 Plant genetics and breeding Potatoes Soil |
| url | http://hdl.handle.net/20.500.11939/6450 https://link.springer.com/journal/10123/volumes-and-issues |
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