Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection
Alternaria brown spot (ABS) is a serious disease affecting susceptible citrus genotypes, which is a strong concern regarding citrus breeding programs. Resistance is conferred by a recessive locus (ABSr) previously located by our group within a 3.3 Mb genome region near the centromere in chromosome I...
| Main Authors: | , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Frontiers Media
2020
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11939/6449 https://www.frontiersin.org/articles/10.3389/fpls.2016.01948/full |
| _version_ | 1855492086327934976 |
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| author | Cuenca, José Aleza, Pablo García-Lor, Andrés Ollitrault, Patrick Navarro, Luis |
| author_browse | Aleza, Pablo Cuenca, José García-Lor, Andrés Navarro, Luis Ollitrault, Patrick |
| author_facet | Cuenca, José Aleza, Pablo García-Lor, Andrés Ollitrault, Patrick Navarro, Luis |
| author_sort | Cuenca, José |
| collection | ReDivia |
| description | Alternaria brown spot (ABS) is a serious disease affecting susceptible citrus genotypes, which is a strong concern regarding citrus breeding programs. Resistance is conferred by a recessive locus (ABSr) previously located by our group within a 3.3 Mb genome region near the centromere in chromosome III. This work addresses fine-linkage mapping of this region for identifying candidate resistance genes and develops new molecular markers for ABS-resistance effective marker-assisted selection (MAS). Markers closely linked to ABSr locus were used for fine mapping using a 268-segregating diploid progeny derived from a heterozygous susceptible × resistant cross. Fine mapping limited the genomic region containing the ABSr resistance gene to 366 kb, flanked by markers at 0.4 and 0.7 cM. This region contains nine genes related to pathogen resistance. Among them, eight are resistance (R) gene homologs, with two of them harboring a serine/threonine protein kinase domain. These two genes along with a gene encoding a S-adenosyl-L-methionine-dependent-methyltransferase protein, should be considered as strong candidates for ABS-resistance. Moreover, the closest SNP was genotyped in 40 citrus varieties, revealing very high association with the resistant/susceptible phenotype. This new marker is currently used in our citrus breeding program for ABS-resistant parent and cultivar selection, at diploid, triploid and tetraploid level. |
| format | Artículo |
| id | ReDivia6449 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | ReDivia64492025-04-25T14:47:07Z Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection Cuenca, José Aleza, Pablo García-Lor, Andrés Ollitrault, Patrick Navarro, Luis Fungal disease resistence H20 Plant diseases Mandarins Gene mapping Molecular markers Alternaria alternata Alternaria brown spot (ABS) is a serious disease affecting susceptible citrus genotypes, which is a strong concern regarding citrus breeding programs. Resistance is conferred by a recessive locus (ABSr) previously located by our group within a 3.3 Mb genome region near the centromere in chromosome III. This work addresses fine-linkage mapping of this region for identifying candidate resistance genes and develops new molecular markers for ABS-resistance effective marker-assisted selection (MAS). Markers closely linked to ABSr locus were used for fine mapping using a 268-segregating diploid progeny derived from a heterozygous susceptible × resistant cross. Fine mapping limited the genomic region containing the ABSr resistance gene to 366 kb, flanked by markers at 0.4 and 0.7 cM. This region contains nine genes related to pathogen resistance. Among them, eight are resistance (R) gene homologs, with two of them harboring a serine/threonine protein kinase domain. These two genes along with a gene encoding a S-adenosyl-L-methionine-dependent-methyltransferase protein, should be considered as strong candidates for ABS-resistance. Moreover, the closest SNP was genotyped in 40 citrus varieties, revealing very high association with the resistant/susceptible phenotype. This new marker is currently used in our citrus breeding program for ABS-resistant parent and cultivar selection, at diploid, triploid and tetraploid level. 2020-05-19T16:39:40Z 2020-05-19T16:39:40Z 2016 article publishedVersion Cuenca, J., Aleza, P., Garcia-Lor, A., Ollitrault, P., & Navarro, L. (2016). Fine mapping for identification of citrus alternaria brown spot candidate resistance genes and development of new SNP markers for marker-assisted selection. Frontiers in plant science, 7, 1948. 1664-462X http://hdl.handle.net/20.500.11939/6449 10.3389/fpls.2016.01948 https://www.frontiersin.org/articles/10.3389/fpls.2016.01948/full en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Frontiers Media electronico |
| spellingShingle | Fungal disease resistence H20 Plant diseases Mandarins Gene mapping Molecular markers Alternaria alternata Cuenca, José Aleza, Pablo García-Lor, Andrés Ollitrault, Patrick Navarro, Luis Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection |
| title | Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection |
| title_full | Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection |
| title_fullStr | Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection |
| title_full_unstemmed | Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection |
| title_short | Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection |
| title_sort | fine mapping for identification of citrus alternaria brown spot candidate resistance genes and development of new snp markers for marker assisted selection |
| topic | Fungal disease resistence H20 Plant diseases Mandarins Gene mapping Molecular markers Alternaria alternata |
| url | http://hdl.handle.net/20.500.11939/6449 https://www.frontiersin.org/articles/10.3389/fpls.2016.01948/full |
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