Aphid Transmission Alters the Genomic and Defective RNA Populations of Citrus tristeza virus Isolates
A total of 14 Spanish isolates of Citrus tristeza virus (CTV) and 1 isolate from Japan were transmitted by Aphis gossypii, and the subisolates obtained were compared with the source isolates for symptom expression and double-stranded RNA (dsRNA) pattern. Of the 14 Spanish isolates, 9 showed alte...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
The American Phytopathological Society (APS)
2020
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6443 https://apsjournals.apsnet.org/doi/10.1094/PHYTO.2000.90.2.134 |
| _version_ | 1855492085071740928 |
|---|---|
| author | Albiach-Martí, María R. Guerri, José Hermoso-De-Mendoza, Alfonso Laigret, F. Ballester-Olmos, José F. Moreno, Pedro |
| author_browse | Albiach-Martí, María R. Ballester-Olmos, José F. Guerri, José Hermoso-De-Mendoza, Alfonso Laigret, F. Moreno, Pedro |
| author_facet | Albiach-Martí, María R. Guerri, José Hermoso-De-Mendoza, Alfonso Laigret, F. Ballester-Olmos, José F. Moreno, Pedro |
| author_sort | Albiach-Martí, María R. |
| collection | ReDivia |
| description | A total of 14 Spanish isolates of Citrus tristeza virus (CTV) and 1 isolate
from Japan were transmitted by Aphis gossypii, and the subisolates obtained
were compared with the source isolates for symptom expression and
double-stranded RNA (dsRNA) pattern. Of the 14 Spanish isolates, 9 showed
altered dsRNA patterns after aphid transmission but only minor variations
in the intensity of symptoms induced on Mexican lime. Northern
blot hybridization with complementary DNA (cDNA) probes corresponding
to both the 5¢ and the 3¢ termini of the CTV genomic RNA (gRNA)
showed that the dsRNA bands that could be used to discriminate between
the dsRNA pattern of the source and the aphid-transmitted isolates were the
replicative forms of defective RNAs (D-RNAs). Conversely, the Japanese
isolate and two subisolates obtained from it by aphid transmission had
the same dsRNA pattern, but one of the subisolates induced milder symptoms
in several hosts. Dot-blot hybridization with cDNA probes representing
several regions of the gRNA showed that most of the aphidtransmitted
isolates differed from the corresponding source isolate by
their hybridization pattern. Our results indicate that aphid transmission
often sorts the populations of gRNA variants and D-RNAs present in
CTV isolates. |
| format | Artículo |
| id | ReDivia6443 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | The American Phytopathological Society (APS) |
| publisherStr | The American Phytopathological Society (APS) |
| record_format | dspace |
| spelling | ReDivia64432025-04-25T14:47:06Z Aphid Transmission Alters the Genomic and Defective RNA Populations of Citrus tristeza virus Isolates Albiach-Martí, María R. Guerri, José Hermoso-De-Mendoza, Alfonso Laigret, F. Ballester-Olmos, José F. Moreno, Pedro Graft inoculation H20 Plant diseases A total of 14 Spanish isolates of Citrus tristeza virus (CTV) and 1 isolate from Japan were transmitted by Aphis gossypii, and the subisolates obtained were compared with the source isolates for symptom expression and double-stranded RNA (dsRNA) pattern. Of the 14 Spanish isolates, 9 showed altered dsRNA patterns after aphid transmission but only minor variations in the intensity of symptoms induced on Mexican lime. Northern blot hybridization with complementary DNA (cDNA) probes corresponding to both the 5¢ and the 3¢ termini of the CTV genomic RNA (gRNA) showed that the dsRNA bands that could be used to discriminate between the dsRNA pattern of the source and the aphid-transmitted isolates were the replicative forms of defective RNAs (D-RNAs). Conversely, the Japanese isolate and two subisolates obtained from it by aphid transmission had the same dsRNA pattern, but one of the subisolates induced milder symptoms in several hosts. Dot-blot hybridization with cDNA probes representing several regions of the gRNA showed that most of the aphidtransmitted isolates differed from the corresponding source isolate by their hybridization pattern. Our results indicate that aphid transmission often sorts the populations of gRNA variants and D-RNAs present in CTV isolates. 2020-05-14T14:39:33Z 2020-05-14T14:39:33Z 2000 article publishedVersion Albiach-Martí, M. R., Guerri, J., de Mendoza, A. H., Laigret, F., Ballester-Olmos, J. F., & Moreno, P. (2000). Aphid transmission alters the genomic and defective RNA populations of citrus tristeza virus isolates. Phytopathology, 90(2), 134-138. 0031-949X http://hdl.handle.net/20.500.11939/6443 10.1094/PHYTO.2000.90.2.134 https://apsjournals.apsnet.org/doi/10.1094/PHYTO.2000.90.2.134 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ The American Phytopathological Society (APS) electronico |
| spellingShingle | Graft inoculation H20 Plant diseases Albiach-Martí, María R. Guerri, José Hermoso-De-Mendoza, Alfonso Laigret, F. Ballester-Olmos, José F. Moreno, Pedro Aphid Transmission Alters the Genomic and Defective RNA Populations of Citrus tristeza virus Isolates |
| title | Aphid Transmission Alters the Genomic and Defective RNA Populations of Citrus tristeza virus Isolates |
| title_full | Aphid Transmission Alters the Genomic and Defective RNA Populations of Citrus tristeza virus Isolates |
| title_fullStr | Aphid Transmission Alters the Genomic and Defective RNA Populations of Citrus tristeza virus Isolates |
| title_full_unstemmed | Aphid Transmission Alters the Genomic and Defective RNA Populations of Citrus tristeza virus Isolates |
| title_short | Aphid Transmission Alters the Genomic and Defective RNA Populations of Citrus tristeza virus Isolates |
| title_sort | aphid transmission alters the genomic and defective rna populations of citrus tristeza virus isolates |
| topic | Graft inoculation H20 Plant diseases |
| url | http://hdl.handle.net/20.500.11939/6443 https://apsjournals.apsnet.org/doi/10.1094/PHYTO.2000.90.2.134 |
| work_keys_str_mv | AT albiachmartimariar aphidtransmissionaltersthegenomicanddefectivernapopulationsofcitrustristezavirusisolates AT guerrijose aphidtransmissionaltersthegenomicanddefectivernapopulationsofcitrustristezavirusisolates AT hermosodemendozaalfonso aphidtransmissionaltersthegenomicanddefectivernapopulationsofcitrustristezavirusisolates AT laigretf aphidtransmissionaltersthegenomicanddefectivernapopulationsofcitrustristezavirusisolates AT ballesterolmosjosef aphidtransmissionaltersthegenomicanddefectivernapopulationsofcitrustristezavirusisolates AT morenopedro aphidtransmissionaltersthegenomicanddefectivernapopulationsofcitrustristezavirusisolates |