A single injection of corifollitropin alfa supplemented with human chorionic gonadotropin increases follicular recruitment and transferable embryos in the rabbit
Superovulation protocols are designed to achieve maximum embryo yields. Nevertheless, ovarian response control and the quality of obtained embryos are still a challenge. On the other hand, to save the superovulated embryos until their subsequent use, it is usual to cryopreserve them, so it is also c...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Blackwell Verlag GmbH
2020
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| Online Access: | http://hdl.handle.net/20.500.11939/6441 https://onlinelibrary.wiley.com/doi/abs/10.1111/rda.13411 |
| _version_ | 1855492084658601984 |
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| author | Viudes-De-Castro, María P. Marco-Jiménez, Francisco Más Pellicer, Alba García-Domínguez, Ximo Talavan, Amparo Salvador Vicente, José |
| author_browse | García-Domínguez, Ximo Marco-Jiménez, Francisco Más Pellicer, Alba Salvador Vicente, José Talavan, Amparo Viudes-De-Castro, María P. |
| author_facet | Viudes-De-Castro, María P. Marco-Jiménez, Francisco Más Pellicer, Alba García-Domínguez, Ximo Talavan, Amparo Salvador Vicente, José |
| author_sort | Viudes-De-Castro, María P. |
| collection | ReDivia |
| description | Superovulation protocols are designed to achieve maximum embryo yields. Nevertheless, ovarian response control and the quality of obtained embryos are still a challenge. On the other hand, to save the superovulated embryos until their subsequent use, it is usual to cryopreserve them, so it is also crucial to assess their cryotolerance. The aim of this study was to compare the efficacy of a single injection of corifollitropin alfa (FSH‐CTP) alone or supplemented with human chorionic gonadotropin (hCG) and to determine the impact of this stimulation on in vitro and in vivo development of fresh or devitrified embryos. Our outcomes showed that ovulation rate and recovered embryos were significantly increased when hCG was used. In vitro development of fresh and devitrified embryos and survival at birth were not significantly affected by superstimulation treatment. Results of this study suggest that a single injection of long‐acting FSH‐CTP supplemented with hCG can be effectively used in rabbits to elicit an increase in ovulation rate and number of recovered embryos. Furthermore, we demonstrated that hCG supplementation had no negative effects in embryo cryosurvival and development, showing similar survival rate at birth than FSH‐CTP alone group. |
| format | Artículo |
| id | ReDivia6441 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Blackwell Verlag GmbH |
| publisherStr | Blackwell Verlag GmbH |
| record_format | dspace |
| spelling | ReDivia64412025-04-25T14:47:06Z A single injection of corifollitropin alfa supplemented with human chorionic gonadotropin increases follicular recruitment and transferable embryos in the rabbit Viudes-De-Castro, María P. Marco-Jiménez, Francisco Más Pellicer, Alba García-Domínguez, Ximo Talavan, Amparo Salvador Vicente, José L53 Animal physiology - Reproduction Superovulation protocols are designed to achieve maximum embryo yields. Nevertheless, ovarian response control and the quality of obtained embryos are still a challenge. On the other hand, to save the superovulated embryos until their subsequent use, it is usual to cryopreserve them, so it is also crucial to assess their cryotolerance. The aim of this study was to compare the efficacy of a single injection of corifollitropin alfa (FSH‐CTP) alone or supplemented with human chorionic gonadotropin (hCG) and to determine the impact of this stimulation on in vitro and in vivo development of fresh or devitrified embryos. Our outcomes showed that ovulation rate and recovered embryos were significantly increased when hCG was used. In vitro development of fresh and devitrified embryos and survival at birth were not significantly affected by superstimulation treatment. Results of this study suggest that a single injection of long‐acting FSH‐CTP supplemented with hCG can be effectively used in rabbits to elicit an increase in ovulation rate and number of recovered embryos. Furthermore, we demonstrated that hCG supplementation had no negative effects in embryo cryosurvival and development, showing similar survival rate at birth than FSH‐CTP alone group. 2020-05-14T11:11:37Z 2020-05-14T11:11:37Z 2019 article acceptedVersion Viudes‐de‐Castro, M. P., Marco‐Jiménez, F., Más Pellicer, A., García‐Domínguez, X., Talaván, A. M., & Vicente, J. S. (2019). A single injection of corifollitropin alfa supplemented with human chorionic gonadotropin increases follicular recruitment and transferable embryos in the rabbit. Reproduction in Domestic Animals, 54(4), 696-701. 1439-0531 http://hdl.handle.net/20.500.11939/6441 10.1111/rda.13411 https://onlinelibrary.wiley.com/doi/abs/10.1111/rda.13411 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Blackwell Verlag GmbH electronico |
| spellingShingle | L53 Animal physiology - Reproduction Viudes-De-Castro, María P. Marco-Jiménez, Francisco Más Pellicer, Alba García-Domínguez, Ximo Talavan, Amparo Salvador Vicente, José A single injection of corifollitropin alfa supplemented with human chorionic gonadotropin increases follicular recruitment and transferable embryos in the rabbit |
| title | A single injection of corifollitropin alfa supplemented with human chorionic gonadotropin increases follicular recruitment and transferable embryos in the rabbit |
| title_full | A single injection of corifollitropin alfa supplemented with human chorionic gonadotropin increases follicular recruitment and transferable embryos in the rabbit |
| title_fullStr | A single injection of corifollitropin alfa supplemented with human chorionic gonadotropin increases follicular recruitment and transferable embryos in the rabbit |
| title_full_unstemmed | A single injection of corifollitropin alfa supplemented with human chorionic gonadotropin increases follicular recruitment and transferable embryos in the rabbit |
| title_short | A single injection of corifollitropin alfa supplemented with human chorionic gonadotropin increases follicular recruitment and transferable embryos in the rabbit |
| title_sort | single injection of corifollitropin alfa supplemented with human chorionic gonadotropin increases follicular recruitment and transferable embryos in the rabbit |
| topic | L53 Animal physiology - Reproduction |
| url | http://hdl.handle.net/20.500.11939/6441 https://onlinelibrary.wiley.com/doi/abs/10.1111/rda.13411 |
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