Isolation and culture of strawberry protoplasts and field evaluation of regenerated plants
Protoplasts are an useful biotechnological tool for plant improvement. In strawberry, very few studies on protoplast technology have been carried out. In this investigation, a procedure for the isolation and culture of strawberry protoplasts, cv. ‘Chandler’, has been developed. The effect of several...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6439 https://www.sciencedirect.com/science/article/abs/pii/S0304423819304418#! |
| _version_ | 1855492084285308928 |
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| author | Barceló, Marta wallin, Anita Medina, Juan J. Gil-Ariza, David J. López-Casado, Gloria Juárez, José Sánchez-Sevilla, José F. López-Encina, Carlos López-Aranda, José M. Mercado, José A. Pliego-Alfaro, Fernando |
| author_browse | Barceló, Marta Gil-Ariza, David J. Juárez, José López-Aranda, José M. López-Casado, Gloria López-Encina, Carlos Medina, Juan J. Mercado, José A. Pliego-Alfaro, Fernando Sánchez-Sevilla, José F. wallin, Anita |
| author_facet | Barceló, Marta wallin, Anita Medina, Juan J. Gil-Ariza, David J. López-Casado, Gloria Juárez, José Sánchez-Sevilla, José F. López-Encina, Carlos López-Aranda, José M. Mercado, José A. Pliego-Alfaro, Fernando |
| author_sort | Barceló, Marta |
| collection | ReDivia |
| description | Protoplasts are an useful biotechnological tool for plant improvement. In strawberry, very few studies on protoplast technology have been carried out. In this investigation, a procedure for the isolation and culture of strawberry protoplasts, cv. ‘Chandler’, has been developed. The effect of several factors affecting the successful isolation of protoplasts and formation of microcalli, e.g. explant source, washing procedure, hormonal composition of the culture medium and protoplast density, were evaluated. For shoot regeneration, microcalli derived from isolated protoplasts were transferred to MS medium supplemented with 0.2 mg l−1 NAA and either 5 mg l−1 BA or 3 mg l−1 TDZ, obtaining a similar regeneration rate, 17%, in both media. Twenty-one independent protoclones were transferred to field conditions for agronomic evaluation. Significant alterations in the growth habit, density of foliage, leaf color and leaf morphology were detected in some lines. Fruit yield was significantly reduced in 15 out of the 21 protoclones evaluated due to a reduction in fruit weight and/or the number of fruits. Ploidy level was unaffected in a sample of 6 lines selected at random; however, a study of genetic stability by using 10 EST-SSR markers showed genetic alterations in all the lines analyzed. Despite the high rate of somaclonal variation detected in the protoclones, some of the lines displayed an agronomical behavior similar to control plants, indicating that this protocol could be useful for genetic improvement in this species. |
| format | Artículo |
| id | ReDivia6439 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | ReDivia64392025-04-25T14:47:06Z Isolation and culture of strawberry protoplasts and field evaluation of regenerated plants Barceló, Marta wallin, Anita Medina, Juan J. Gil-Ariza, David J. López-Casado, Gloria Juárez, José Sánchez-Sevilla, José F. López-Encina, Carlos López-Aranda, José M. Mercado, José A. Pliego-Alfaro, Fernando Agronomic evaluation Protoplast isolation Protoplast regeneration F30 Plant genetics and breeding Fragaria ananassa Somaclonal variation Strawberry (tree) Protoplasts are an useful biotechnological tool for plant improvement. In strawberry, very few studies on protoplast technology have been carried out. In this investigation, a procedure for the isolation and culture of strawberry protoplasts, cv. ‘Chandler’, has been developed. The effect of several factors affecting the successful isolation of protoplasts and formation of microcalli, e.g. explant source, washing procedure, hormonal composition of the culture medium and protoplast density, were evaluated. For shoot regeneration, microcalli derived from isolated protoplasts were transferred to MS medium supplemented with 0.2 mg l−1 NAA and either 5 mg l−1 BA or 3 mg l−1 TDZ, obtaining a similar regeneration rate, 17%, in both media. Twenty-one independent protoclones were transferred to field conditions for agronomic evaluation. Significant alterations in the growth habit, density of foliage, leaf color and leaf morphology were detected in some lines. Fruit yield was significantly reduced in 15 out of the 21 protoclones evaluated due to a reduction in fruit weight and/or the number of fruits. Ploidy level was unaffected in a sample of 6 lines selected at random; however, a study of genetic stability by using 10 EST-SSR markers showed genetic alterations in all the lines analyzed. Despite the high rate of somaclonal variation detected in the protoclones, some of the lines displayed an agronomical behavior similar to control plants, indicating that this protocol could be useful for genetic improvement in this species. 2020-05-14T10:54:42Z 2020-05-14T10:54:42Z 2019 article Barceló, M., Wallin, A., Medina, J. J., Gil-Ariza, D. J., López-Casado, G., Juarez, J., ... & Pliego-Alfaro, F. (2019). Isolation and culture of strawberry protoplasts and field evaluation of regenerated plants. Scientia Horticulturae, 256, 108552. 0304-4238 http://hdl.handle.net/20.500.11939/6439 10.1016/j.scienta.2019.108552 https://www.sciencedirect.com/science/article/abs/pii/S0304423819304418#! en closedAccess Elsevier electronico |
| spellingShingle | Agronomic evaluation Protoplast isolation Protoplast regeneration F30 Plant genetics and breeding Fragaria ananassa Somaclonal variation Strawberry (tree) Barceló, Marta wallin, Anita Medina, Juan J. Gil-Ariza, David J. López-Casado, Gloria Juárez, José Sánchez-Sevilla, José F. López-Encina, Carlos López-Aranda, José M. Mercado, José A. Pliego-Alfaro, Fernando Isolation and culture of strawberry protoplasts and field evaluation of regenerated plants |
| title | Isolation and culture of strawberry protoplasts and field evaluation of regenerated plants |
| title_full | Isolation and culture of strawberry protoplasts and field evaluation of regenerated plants |
| title_fullStr | Isolation and culture of strawberry protoplasts and field evaluation of regenerated plants |
| title_full_unstemmed | Isolation and culture of strawberry protoplasts and field evaluation of regenerated plants |
| title_short | Isolation and culture of strawberry protoplasts and field evaluation of regenerated plants |
| title_sort | isolation and culture of strawberry protoplasts and field evaluation of regenerated plants |
| topic | Agronomic evaluation Protoplast isolation Protoplast regeneration F30 Plant genetics and breeding Fragaria ananassa Somaclonal variation Strawberry (tree) |
| url | http://hdl.handle.net/20.500.11939/6439 https://www.sciencedirect.com/science/article/abs/pii/S0304423819304418#! |
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