A comparative study of viral infectivity, accumulation and symptoms induced by broad bean wilt virus 1 isolate

Broad bean wilt virus 1 (BBWV-1, genus Fabavirus, family Secoviridae) is a bipartite positive-sense single-stranded RNA virus distributed worldwide infecting many herbaceous species. Until now, scarce information regarding biological properties of BBWV-1 isolates is available. This work shows a comp...

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Main Authors: Carpino, Caterina, Elvira-González, Laura, Rubio, Luis, Peri, Ezio, Davino, Salvatore, Galipienso, Luis
Format: article
Language:Inglés
Published: Springer 2020
Subjects:
Online Access:http://hdl.handle.net/20.500.11939/6435
https://link.springer.com/article/10.1007/s42161-018-00198-y
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author Carpino, Caterina
Elvira-González, Laura
Rubio, Luis
Peri, Ezio
Davino, Salvatore
Galipienso, Luis
author_browse Carpino, Caterina
Davino, Salvatore
Elvira-González, Laura
Galipienso, Luis
Peri, Ezio
Rubio, Luis
author_facet Carpino, Caterina
Elvira-González, Laura
Rubio, Luis
Peri, Ezio
Davino, Salvatore
Galipienso, Luis
author_sort Carpino, Caterina
collection ReDivia
description Broad bean wilt virus 1 (BBWV-1, genus Fabavirus, family Secoviridae) is a bipartite positive-sense single-stranded RNA virus distributed worldwide infecting many herbaceous species. Until now, scarce information regarding biological properties of BBWV-1 isolates is available. This work shows a comparative study on virus infectivity (proportion of infected plants over inoculated plants), virus accumulation and symptoms induced by four genetically different BBWV-1 isolates (Ben, B41/99, NSRV and PV0548) which were mechanically inoculated on several herbaceous hosts. The four BBWV-1 isolates infected broad bean, tomato, pepper and Nicotiana benthamiana plants, whereas none of them infected cucumber, common bean and melon. Infectivity ranged from 40 to 60% in tomato and from 75% to 95% in pepper, whereas it was 100% in broad bean and N. benthamiana for the four BBWV-1 isolates. Symptoms showed differences depending on the host plant, the viral isolate and the infection time. Virus accumulation was determined in broad bean and pepper plants and showed differences among host species and BBWV-1 isolates. No association between plant symptom manifestation and viral titre was observed.
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spelling ReDivia64352025-04-25T14:47:04Z A comparative study of viral infectivity, accumulation and symptoms induced by broad bean wilt virus 1 isolate Carpino, Caterina Elvira-González, Laura Rubio, Luis Peri, Ezio Davino, Salvatore Galipienso, Luis BBWV-1 Secoviridae Fabavirus Symptomatology RT-qPCR H20 Plant diseases Infectivity Broad bean wilt virus 1 (BBWV-1, genus Fabavirus, family Secoviridae) is a bipartite positive-sense single-stranded RNA virus distributed worldwide infecting many herbaceous species. Until now, scarce information regarding biological properties of BBWV-1 isolates is available. This work shows a comparative study on virus infectivity (proportion of infected plants over inoculated plants), virus accumulation and symptoms induced by four genetically different BBWV-1 isolates (Ben, B41/99, NSRV and PV0548) which were mechanically inoculated on several herbaceous hosts. The four BBWV-1 isolates infected broad bean, tomato, pepper and Nicotiana benthamiana plants, whereas none of them infected cucumber, common bean and melon. Infectivity ranged from 40 to 60% in tomato and from 75% to 95% in pepper, whereas it was 100% in broad bean and N. benthamiana for the four BBWV-1 isolates. Symptoms showed differences depending on the host plant, the viral isolate and the infection time. Virus accumulation was determined in broad bean and pepper plants and showed differences among host species and BBWV-1 isolates. No association between plant symptom manifestation and viral titre was observed. 2020-05-11T16:23:13Z 2020-05-11T16:23:13Z 2019 article acceptedVersion publishedVersion Carpino, C., Elvira-González, L., Rubio, L., Peri, E., Davino, S., & Galipienso, L. (2019). A comparative study of viral infectivity, accumulation and symptoms induced by broad bean wilt virus 1 isolates. Journal of Plant Pathology, 101(2), 275-281. 1125-4653 http://hdl.handle.net/20.500.11939/6435 10.1007/s42161-018-00198-y https://link.springer.com/article/10.1007/s42161-018-00198-y en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Springer electronico
spellingShingle BBWV-1
Secoviridae
Fabavirus
Symptomatology
RT-qPCR
H20 Plant diseases
Infectivity
Carpino, Caterina
Elvira-González, Laura
Rubio, Luis
Peri, Ezio
Davino, Salvatore
Galipienso, Luis
A comparative study of viral infectivity, accumulation and symptoms induced by broad bean wilt virus 1 isolate
title A comparative study of viral infectivity, accumulation and symptoms induced by broad bean wilt virus 1 isolate
title_full A comparative study of viral infectivity, accumulation and symptoms induced by broad bean wilt virus 1 isolate
title_fullStr A comparative study of viral infectivity, accumulation and symptoms induced by broad bean wilt virus 1 isolate
title_full_unstemmed A comparative study of viral infectivity, accumulation and symptoms induced by broad bean wilt virus 1 isolate
title_short A comparative study of viral infectivity, accumulation and symptoms induced by broad bean wilt virus 1 isolate
title_sort comparative study of viral infectivity accumulation and symptoms induced by broad bean wilt virus 1 isolate
topic BBWV-1
Secoviridae
Fabavirus
Symptomatology
RT-qPCR
H20 Plant diseases
Infectivity
url http://hdl.handle.net/20.500.11939/6435
https://link.springer.com/article/10.1007/s42161-018-00198-y
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