Precocious flowering of juvenile citrus induced by a viral vector based on Citrus leaf blotch virus: a new tool for genetics and breeding
The long juvenile period of citrus trees (often more than 6 years) has hindered genetic improvement by traditional breeding methods and genetic studies. In this work, we have developed a biotechnology tool to promote transition from the vegetative to the reproductive phase in juvenile citrus plants...
| Main Authors: | , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd
2020
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11939/6423 https://onlinelibrary.wiley.com/doi/full/10.1111/pbi.12555 |
| _version_ | 1855492081155309568 |
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| author | Velázquez, Karelia Agüero, Jesús Vives, María C. Aleza, Pablo Pina, José A. Moreno, Pedro Navarro, Luis Guerri, José |
| author_browse | Agüero, Jesús Aleza, Pablo Guerri, José Moreno, Pedro Navarro, Luis Pina, José A. Velázquez, Karelia Vives, María C. |
| author_facet | Velázquez, Karelia Agüero, Jesús Vives, María C. Aleza, Pablo Pina, José A. Moreno, Pedro Navarro, Luis Guerri, José |
| author_sort | Velázquez, Karelia |
| collection | ReDivia |
| description | The long juvenile period of citrus trees (often more than 6 years) has hindered genetic improvement by traditional breeding methods and genetic studies. In this work, we have developed a biotechnology tool to promote transition from the vegetative to the reproductive phase in juvenile citrus plants by expression of the Arabidopsis thaliana or citrus FLOWERING LOCUS T (FT) genes using a Citrus leaf blotch virus-based vector (clbvINpr-AtFT and clbvINpr-CiFT, respectively). Citrus plants of different genotypes graft inoculated with either of these vectors started flowering within 4–6 months, with no alteration of the plant architecture, leaf, flower or fruit morphology in comparison with noninoculated adult plants. The vector did not integrate in or recombine with the plant genome nor was it pollen or vector transmissible, albeit seed transmission at low rate was detected. The clbvINpr-AtFT is very stable, and flowering was observed over a period of at least 5 years. Precocious flowering of juvenile citrus plants after vector infection provides a helpful and safe tool to dramatically speed up genetic studies and breeding programmes. |
| format | Artículo |
| id | ReDivia6423 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd |
| publisherStr | Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd |
| record_format | dspace |
| spelling | ReDivia64232025-04-25T14:47:02Z Precocious flowering of juvenile citrus induced by a viral vector based on Citrus leaf blotch virus: a new tool for genetics and breeding Velázquez, Karelia Agüero, Jesús Vives, María C. Aleza, Pablo Pina, José A. Moreno, Pedro Navarro, Luis Guerri, José Citrus vector Speed up genetic studies Early flowering in juvenile citrus plant F30 Plant genetics and breeding The long juvenile period of citrus trees (often more than 6 years) has hindered genetic improvement by traditional breeding methods and genetic studies. In this work, we have developed a biotechnology tool to promote transition from the vegetative to the reproductive phase in juvenile citrus plants by expression of the Arabidopsis thaliana or citrus FLOWERING LOCUS T (FT) genes using a Citrus leaf blotch virus-based vector (clbvINpr-AtFT and clbvINpr-CiFT, respectively). Citrus plants of different genotypes graft inoculated with either of these vectors started flowering within 4–6 months, with no alteration of the plant architecture, leaf, flower or fruit morphology in comparison with noninoculated adult plants. The vector did not integrate in or recombine with the plant genome nor was it pollen or vector transmissible, albeit seed transmission at low rate was detected. The clbvINpr-AtFT is very stable, and flowering was observed over a period of at least 5 years. Precocious flowering of juvenile citrus plants after vector infection provides a helpful and safe tool to dramatically speed up genetic studies and breeding programmes. 2020-05-06T11:02:08Z 2020-05-06T11:02:08Z 2016 article publishedVersion Velázquez, K., Agüero, J., Vives, M. C., Aleza, P., Pina, J. A., Moreno, P. et al. (2016). Precocious flowering of juvenile citrus induced by a viral vector based on Citrus leaf blotch virus: a new tool for genetics and breeding. Plant biotechnology journal, 14(10), 1976-1985. 1467-7652 http://hdl.handle.net/20.500.11939/6423 10.1111/pbi.12555 https://onlinelibrary.wiley.com/doi/full/10.1111/pbi.12555 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd electronico |
| spellingShingle | Citrus vector Speed up genetic studies Early flowering in juvenile citrus plant F30 Plant genetics and breeding Velázquez, Karelia Agüero, Jesús Vives, María C. Aleza, Pablo Pina, José A. Moreno, Pedro Navarro, Luis Guerri, José Precocious flowering of juvenile citrus induced by a viral vector based on Citrus leaf blotch virus: a new tool for genetics and breeding |
| title | Precocious flowering of juvenile citrus induced by a viral vector based on Citrus leaf blotch virus: a new tool for genetics and breeding |
| title_full | Precocious flowering of juvenile citrus induced by a viral vector based on Citrus leaf blotch virus: a new tool for genetics and breeding |
| title_fullStr | Precocious flowering of juvenile citrus induced by a viral vector based on Citrus leaf blotch virus: a new tool for genetics and breeding |
| title_full_unstemmed | Precocious flowering of juvenile citrus induced by a viral vector based on Citrus leaf blotch virus: a new tool for genetics and breeding |
| title_short | Precocious flowering of juvenile citrus induced by a viral vector based on Citrus leaf blotch virus: a new tool for genetics and breeding |
| title_sort | precocious flowering of juvenile citrus induced by a viral vector based on citrus leaf blotch virus a new tool for genetics and breeding |
| topic | Citrus vector Speed up genetic studies Early flowering in juvenile citrus plant F30 Plant genetics and breeding |
| url | http://hdl.handle.net/20.500.11939/6423 https://onlinelibrary.wiley.com/doi/full/10.1111/pbi.12555 |
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