Mutations in the voltage-gated sodium channel gene associated with deltamethrin resistance in commercially sourced Phytoseiulus persimilis
The implementation of Integrated Pest Management in current agricultural practice is a convenient and very effective strategy to keep pest populations under control. The use of biological control agents, such as Phytoseiulus persimilis, is key for the success of such an approach. This predatory mite...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Artículo preliminar |
| Publicado: |
Wiley
2020
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6390 https://onlinelibrary.wiley.com/doi/abs/10.1111/imb.12642 |
| _version_ | 1855492075097686016 |
|---|---|
| author | Benavent-Albarracín, Luís Alonso-Valiente, Miquel Catalán, José Urbaneja, Alberto Davies, T.G. Emyr Williamson, Martin S. González-Cabrera, Joel |
| author_browse | Alonso-Valiente, Miquel Benavent-Albarracín, Luís Catalán, José Davies, T.G. Emyr González-Cabrera, Joel Urbaneja, Alberto Williamson, Martin S. |
| author_facet | Benavent-Albarracín, Luís Alonso-Valiente, Miquel Catalán, José Urbaneja, Alberto Davies, T.G. Emyr Williamson, Martin S. González-Cabrera, Joel |
| author_sort | Benavent-Albarracín, Luís |
| collection | ReDivia |
| description | The implementation of Integrated Pest Management in current agricultural practice is a convenient and very effective strategy to keep pest populations under control. The use of biological control agents, such as Phytoseiulus persimilis, is key for the success of such an approach. This predatory mite is widely used as it is very effective for controlling Tetranychus urticae, one of the most devastating crop pests. Here, we identify several mutations located in the voltage‐gated sodium channel (VGSC) of commercially sourced P. persimilis that correlate with a reduced susceptibility to the pyrethroid deltamethrin. We found that the mites sourced from two different biocontrol product companies have intrinsic genotypic differences that correlate with their phenotype when tested with different concentrations of deltamethrin. Mites from Syngenta Bioline, carrying the mutations M918L and A1536T, were able to survive deltamethrin concentrations of up to 10 ppm, while the mites from Koppert Biological Systems, with the combination M918L, L925V and S1539T, survived treatment with 40 ppm. All of the point mutations identified in the predatory mite samples are located in a particular region of the VGSC, previously proposed as the binding site for this family of pesticides and identified as a ‘hot spot’ for resistance. |
| format | Artículo preliminar |
| id | ReDivia6390 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | ReDivia63902025-04-25T14:46:58Z Mutations in the voltage-gated sodium channel gene associated with deltamethrin resistance in commercially sourced Phytoseiulus persimilis Benavent-Albarracín, Luís Alonso-Valiente, Miquel Catalán, José Urbaneja, Alberto Davies, T.G. Emyr Williamson, Martin S. González-Cabrera, Joel pyrethroids VGSC deltamethrin target site resistance predatory mites P. persimilis H10 Pests of plants Integrated pest management The implementation of Integrated Pest Management in current agricultural practice is a convenient and very effective strategy to keep pest populations under control. The use of biological control agents, such as Phytoseiulus persimilis, is key for the success of such an approach. This predatory mite is widely used as it is very effective for controlling Tetranychus urticae, one of the most devastating crop pests. Here, we identify several mutations located in the voltage‐gated sodium channel (VGSC) of commercially sourced P. persimilis that correlate with a reduced susceptibility to the pyrethroid deltamethrin. We found that the mites sourced from two different biocontrol product companies have intrinsic genotypic differences that correlate with their phenotype when tested with different concentrations of deltamethrin. Mites from Syngenta Bioline, carrying the mutations M918L and A1536T, were able to survive deltamethrin concentrations of up to 10 ppm, while the mites from Koppert Biological Systems, with the combination M918L, L925V and S1539T, survived treatment with 40 ppm. All of the point mutations identified in the predatory mite samples are located in a particular region of the VGSC, previously proposed as the binding site for this family of pesticides and identified as a ‘hot spot’ for resistance. 2020-04-22T10:37:07Z 2020-04-22T10:37:07Z 2020 acceptedVersion Benavent‐Albarracín, L., Alonso, M., Catalán, J., Urbaneja, A., Davies, T. G. E., Williamson, M. S., & González‐Cabrera, J. (2020). Mutations in the voltage‐gated sodium channel gene associated with deltamethrin resistance in commercially sourced Phytoseiulus persimilis. Insect Molecular Biology, 29(4), 373-380. http://hdl.handle.net/20.500.11939/6390 10.1111/imb.12642 https://onlinelibrary.wiley.com/doi/abs/10.1111/imb.12642 info:eu-repo/grantAgreement/MINECO/Programa Estatal de Promoción del Talento y su Empleabilidad/BES2016-077086 info:eu-repo/grantAgreement/MINECO/Programa Estatal de Promoción del Talento y su Empleabilidad/RYC-2013-13834 info:eu-repo/grantAgreement/MINECO/Programa estatal de i+D+i Orientada a los Retos de la Sociedad/CGL2015-65025-R//ESTUDIO DE LOS MECANISMOS DE RESISTENCIA A ACARICIDAS EN VARROA DESTRUCTOR Spanish Ministry of Economy 259 and Competitiveness (BES-2016-077086) Ramón y Cajal Program (RYC-2013- 261 13834) Spanish Ministry of Economy and Competitiveness (CGL2015‐65025‐262 R, MINECO/FEDER, UE) Conselleria d’Agricultura, Pesca i Alimentació de la Generalitat Valenciana Wiley |
| spellingShingle | pyrethroids VGSC deltamethrin target site resistance predatory mites P. persimilis H10 Pests of plants Integrated pest management Benavent-Albarracín, Luís Alonso-Valiente, Miquel Catalán, José Urbaneja, Alberto Davies, T.G. Emyr Williamson, Martin S. González-Cabrera, Joel Mutations in the voltage-gated sodium channel gene associated with deltamethrin resistance in commercially sourced Phytoseiulus persimilis |
| title | Mutations in the voltage-gated sodium channel gene associated with deltamethrin resistance in commercially sourced Phytoseiulus persimilis |
| title_full | Mutations in the voltage-gated sodium channel gene associated with deltamethrin resistance in commercially sourced Phytoseiulus persimilis |
| title_fullStr | Mutations in the voltage-gated sodium channel gene associated with deltamethrin resistance in commercially sourced Phytoseiulus persimilis |
| title_full_unstemmed | Mutations in the voltage-gated sodium channel gene associated with deltamethrin resistance in commercially sourced Phytoseiulus persimilis |
| title_short | Mutations in the voltage-gated sodium channel gene associated with deltamethrin resistance in commercially sourced Phytoseiulus persimilis |
| title_sort | mutations in the voltage gated sodium channel gene associated with deltamethrin resistance in commercially sourced phytoseiulus persimilis |
| topic | pyrethroids VGSC deltamethrin target site resistance predatory mites P. persimilis H10 Pests of plants Integrated pest management |
| url | http://hdl.handle.net/20.500.11939/6390 https://onlinelibrary.wiley.com/doi/abs/10.1111/imb.12642 |
| work_keys_str_mv | AT benaventalbarracinluis mutationsinthevoltagegatedsodiumchannelgeneassociatedwithdeltamethrinresistanceincommerciallysourcedphytoseiuluspersimilis AT alonsovalientemiquel mutationsinthevoltagegatedsodiumchannelgeneassociatedwithdeltamethrinresistanceincommerciallysourcedphytoseiuluspersimilis AT catalanjose mutationsinthevoltagegatedsodiumchannelgeneassociatedwithdeltamethrinresistanceincommerciallysourcedphytoseiuluspersimilis AT urbanejaalberto mutationsinthevoltagegatedsodiumchannelgeneassociatedwithdeltamethrinresistanceincommerciallysourcedphytoseiuluspersimilis AT daviestgemyr mutationsinthevoltagegatedsodiumchannelgeneassociatedwithdeltamethrinresistanceincommerciallysourcedphytoseiuluspersimilis AT williamsonmartins mutationsinthevoltagegatedsodiumchannelgeneassociatedwithdeltamethrinresistanceincommerciallysourcedphytoseiuluspersimilis AT gonzalezcabrerajoel mutationsinthevoltagegatedsodiumchannelgeneassociatedwithdeltamethrinresistanceincommerciallysourcedphytoseiuluspersimilis |