Proteomic characterization of rabbit (Oryctolagus cuniculus) sperm from two different genotypes
The present study was conducted to characterise rabbit sperm proteins focusing on the influence of the genetic origin. Six samples were recovered during two months from five males from genotype A (New Zealand White origin) and five from genotype R (California origin). Sperm proteins were extracted a...
| Autores principales: | , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6374 https://www.sciencedirect.com/science/article/abs/pii/S0093691X1830863X |
| _version_ | 1855492072058912768 |
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| author | Casares-Crespo, Lucía Fernández-Serrano, Paula Viudes-De-Castro, María P. |
| author_browse | Casares-Crespo, Lucía Fernández-Serrano, Paula Viudes-De-Castro, María P. |
| author_facet | Casares-Crespo, Lucía Fernández-Serrano, Paula Viudes-De-Castro, María P. |
| author_sort | Casares-Crespo, Lucía |
| collection | ReDivia |
| description | The present study was conducted to characterise rabbit sperm proteins focusing on the influence of the genetic origin. Six samples were recovered during two months from five males from genotype A (New Zealand White origin) and five from genotype R (California origin). Sperm proteins were extracted and subjected to in-gel digestion nano LC-MS/MS and bioinformatics analysis. The resulting library included 487 identified proteins validated with ≥95% Confidence (unused Score ≥ 1.3). All the identified proteins belonged to Oryctolagus cuniculus taxonomy. These data are available via ProteomeXchange with identifier PXD007989. Only 7 proteins were specifically implicated in reproductive processes according to Gene Ontology annotation. Regarding the comparison of the sperm proteins abundance between genotypes, forty proteins were differentially expressed. Among them, 25 proteins were over-expressed in genotype A, while 15 proteins were over-expressed in genotype R. In conclusion, this study characterizes for the first time rabbit sperm proteins and provides evidence that genotype is related to a specific abundance of spermatozoa proteins. |
| format | Artículo |
| id | ReDivia6374 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | ReDivia63742025-04-25T14:46:55Z Proteomic characterization of rabbit (Oryctolagus cuniculus) sperm from two different genotypes Casares-Crespo, Lucía Fernández-Serrano, Paula Viudes-De-Castro, María P. Proteome LC-MS/MS L10 Animal genetics and breeding Rabbits Spermatozoa Genotypes The present study was conducted to characterise rabbit sperm proteins focusing on the influence of the genetic origin. Six samples were recovered during two months from five males from genotype A (New Zealand White origin) and five from genotype R (California origin). Sperm proteins were extracted and subjected to in-gel digestion nano LC-MS/MS and bioinformatics analysis. The resulting library included 487 identified proteins validated with ≥95% Confidence (unused Score ≥ 1.3). All the identified proteins belonged to Oryctolagus cuniculus taxonomy. These data are available via ProteomeXchange with identifier PXD007989. Only 7 proteins were specifically implicated in reproductive processes according to Gene Ontology annotation. Regarding the comparison of the sperm proteins abundance between genotypes, forty proteins were differentially expressed. Among them, 25 proteins were over-expressed in genotype A, while 15 proteins were over-expressed in genotype R. In conclusion, this study characterizes for the first time rabbit sperm proteins and provides evidence that genotype is related to a specific abundance of spermatozoa proteins. 2020-04-14T16:08:59Z 2020-04-14T16:08:59Z 2019 article acceptedVersion Casares-Crespo, L., Fernández-Serrano, P., & Viudes-de-Castro, M. P. (2019). Proteomic characterization of rabbit (Oryctolagus cuniculus) sperm from two different genotypes. Theriogenology, 128, 140-148. 0093-691X http://hdl.handle.net/20.500.11939/6374 10.1016/j.theriogenology.2019.01.026 https://www.sciencedirect.com/science/article/abs/pii/S0093691X1830863X en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Elsevier electronico |
| spellingShingle | Proteome LC-MS/MS L10 Animal genetics and breeding Rabbits Spermatozoa Genotypes Casares-Crespo, Lucía Fernández-Serrano, Paula Viudes-De-Castro, María P. Proteomic characterization of rabbit (Oryctolagus cuniculus) sperm from two different genotypes |
| title | Proteomic characterization of rabbit (Oryctolagus cuniculus) sperm from two different genotypes |
| title_full | Proteomic characterization of rabbit (Oryctolagus cuniculus) sperm from two different genotypes |
| title_fullStr | Proteomic characterization of rabbit (Oryctolagus cuniculus) sperm from two different genotypes |
| title_full_unstemmed | Proteomic characterization of rabbit (Oryctolagus cuniculus) sperm from two different genotypes |
| title_short | Proteomic characterization of rabbit (Oryctolagus cuniculus) sperm from two different genotypes |
| title_sort | proteomic characterization of rabbit oryctolagus cuniculus sperm from two different genotypes |
| topic | Proteome LC-MS/MS L10 Animal genetics and breeding Rabbits Spermatozoa Genotypes |
| url | http://hdl.handle.net/20.500.11939/6374 https://www.sciencedirect.com/science/article/abs/pii/S0093691X1830863X |
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