A new Capsicum baccatum accession shows tolerance to wild‐type and resistance‐breaking isolates of Tomato spotted wilt virus
Tomato spotted wilt virus (TSWV) causes economically important losses in many crops, worldwide. In pepper (Capsicum annuum), the best method for disease control has been breeding resistant cultivars by introgression of gene Tsw from Capsicum chinense. However, this resistance has two drawbacks: (a)...
| Autores principales: | , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6366 https://onlinelibrary.wiley.com/doi/abs/10.1111/aab.12229 |
| _version_ | 1855032414155309056 |
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| author | Soler, Salvador Debreczeni, Diana E. Vidal, Eduardo Aramburu, Jose López, C. Galipienso, Luis Rubio, Luis |
| author_browse | Aramburu, Jose Debreczeni, Diana E. Galipienso, Luis López, C. Rubio, Luis Soler, Salvador Vidal, Eduardo |
| author_facet | Soler, Salvador Debreczeni, Diana E. Vidal, Eduardo Aramburu, Jose López, C. Galipienso, Luis Rubio, Luis |
| author_sort | Soler, Salvador |
| collection | ReDivia |
| description | Tomato spotted wilt virus (TSWV) causes economically important losses in many crops, worldwide. In pepper (Capsicum annuum), the best method for disease control has been breeding resistant cultivars by introgression of gene Tsw from Capsicum chinense. However, this resistance has two drawbacks: (a) it is not efficient if plants are infected at early growth stages and under prolonged high temperatures, and (b) it is rapidly overcome by TSWV evolution. In this work, we selected and evaluated a new accession from Capsicum baccatum, named PIM26‐1, using a novel approach consisting in measuring how three parameters related to virus infection changed over time, in comparison to a susceptible pepper variety (Negral) and a resistant (with Tsw) accession (PI‐159236): (a) The level of resistance to virus accumulation was estimated as an opposite to absolute fitness, W=er, being r the viral multiplication rate calculated by quantitative RT‐PCR; (b); the level of resistance to virus infection was estimated as the Kaplan–Meier survival time for no infection using DAS‐ELISA to identify TSWV‐infected plants; (c) the level of tolerance was estimated as the Kaplan–Meier survival time for no appearance of severe symptoms. Our results showed that the levels of both resistance parameters against TSWV wild type (WT) and Tsw‐resistance breaking (TBR) isolates were higher in PIM26‐1 than in the susceptible pepper variety Negral and similar to the resistant variety PI‐159236 against the TBR isolate. However, PIM26‐1 showed a very high tolerance (none of the plants developed severe symptoms) to the WT and TBR isolates in contrast to Negral for WT and TBR or PI‐159236 for TBR (most TSWV‐inoculated plants developed severe symptoms). All this indicate that the new accession PIM26‐1 is a good candidate for breeding programmes to avoid damages caused by TSWV TBR isolates in pepper. |
| format | article |
| id | ReDivia6366 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | ReDivia63662025-04-25T14:46:54Z A new Capsicum baccatum accession shows tolerance to wild‐type and resistance‐breaking isolates of Tomato spotted wilt virus Soler, Salvador Debreczeni, Diana E. Vidal, Eduardo Aramburu, Jose López, C. Galipienso, Luis Rubio, Luis TSWV Tospovirus Resistance Kaplan-Meier H20 Plant diseases Plant breeding Pepper Bunyaviridae Fitness (physical) Tomato spotted wilt virus (TSWV) causes economically important losses in many crops, worldwide. In pepper (Capsicum annuum), the best method for disease control has been breeding resistant cultivars by introgression of gene Tsw from Capsicum chinense. However, this resistance has two drawbacks: (a) it is not efficient if plants are infected at early growth stages and under prolonged high temperatures, and (b) it is rapidly overcome by TSWV evolution. In this work, we selected and evaluated a new accession from Capsicum baccatum, named PIM26‐1, using a novel approach consisting in measuring how three parameters related to virus infection changed over time, in comparison to a susceptible pepper variety (Negral) and a resistant (with Tsw) accession (PI‐159236): (a) The level of resistance to virus accumulation was estimated as an opposite to absolute fitness, W=er, being r the viral multiplication rate calculated by quantitative RT‐PCR; (b); the level of resistance to virus infection was estimated as the Kaplan–Meier survival time for no infection using DAS‐ELISA to identify TSWV‐infected plants; (c) the level of tolerance was estimated as the Kaplan–Meier survival time for no appearance of severe symptoms. Our results showed that the levels of both resistance parameters against TSWV wild type (WT) and Tsw‐resistance breaking (TBR) isolates were higher in PIM26‐1 than in the susceptible pepper variety Negral and similar to the resistant variety PI‐159236 against the TBR isolate. However, PIM26‐1 showed a very high tolerance (none of the plants developed severe symptoms) to the WT and TBR isolates in contrast to Negral for WT and TBR or PI‐159236 for TBR (most TSWV‐inoculated plants developed severe symptoms). All this indicate that the new accession PIM26‐1 is a good candidate for breeding programmes to avoid damages caused by TSWV TBR isolates in pepper. 2020-04-14T14:15:22Z 2020-04-14T14:15:22Z 2015 article acceptedVersion Soler, S., Debreczeni, D. E., Vidal, E., Aramburu, J., López, C., Galipienso, L., & Rubio, L. (2015). A new Capsicum baccatum accession shows tolerance to wild‐type and resistance‐breaking isolates of Tomato spotted wilt virus. Annals of applied biology, 167(3), 343-353. 1744-7348 http://hdl.handle.net/20.500.11939/6366 10.1111/aab.12229 https://onlinelibrary.wiley.com/doi/abs/10.1111/aab.12229 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Wiley electronico |
| spellingShingle | TSWV Tospovirus Resistance Kaplan-Meier H20 Plant diseases Plant breeding Pepper Bunyaviridae Fitness (physical) Soler, Salvador Debreczeni, Diana E. Vidal, Eduardo Aramburu, Jose López, C. Galipienso, Luis Rubio, Luis A new Capsicum baccatum accession shows tolerance to wild‐type and resistance‐breaking isolates of Tomato spotted wilt virus |
| title | A new Capsicum baccatum accession shows tolerance to wild‐type and resistance‐breaking isolates of Tomato spotted wilt virus |
| title_full | A new Capsicum baccatum accession shows tolerance to wild‐type and resistance‐breaking isolates of Tomato spotted wilt virus |
| title_fullStr | A new Capsicum baccatum accession shows tolerance to wild‐type and resistance‐breaking isolates of Tomato spotted wilt virus |
| title_full_unstemmed | A new Capsicum baccatum accession shows tolerance to wild‐type and resistance‐breaking isolates of Tomato spotted wilt virus |
| title_short | A new Capsicum baccatum accession shows tolerance to wild‐type and resistance‐breaking isolates of Tomato spotted wilt virus |
| title_sort | new capsicum baccatum accession shows tolerance to wild type and resistance breaking isolates of tomato spotted wilt virus |
| topic | TSWV Tospovirus Resistance Kaplan-Meier H20 Plant diseases Plant breeding Pepper Bunyaviridae Fitness (physical) |
| url | http://hdl.handle.net/20.500.11939/6366 https://onlinelibrary.wiley.com/doi/abs/10.1111/aab.12229 |
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