PdMFS1 Transporter Contributes to Penicilliun digitatum Fungicide Resistance and Fungal Virulence during Citrus Fruit Infection
A new Penicillium digitatum major facilitator superfamily (MFS) transporter (PdMFS1) was identified and functionally characterized in order to shed more light on the mechanisms underlying fungicide resistance. PdMFS1 can play an important role in the intensification of resistance to fungicides norma...
| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2020
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/6300 https://www.mdpi.com/2309-608X/5/4/100 |
| _version_ | 1855032404209565696 |
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| author | De-Ramón-Carbonell, Marta López-Pérez, Mario Gonzalez-Candelas, Luis Sánchez-Torres, Paloma |
| author_browse | De-Ramón-Carbonell, Marta Gonzalez-Candelas, Luis López-Pérez, Mario Sánchez-Torres, Paloma |
| author_facet | De-Ramón-Carbonell, Marta López-Pérez, Mario Gonzalez-Candelas, Luis Sánchez-Torres, Paloma |
| author_sort | De-Ramón-Carbonell, Marta |
| collection | ReDivia |
| description | A new Penicillium digitatum major facilitator superfamily (MFS) transporter (PdMFS1) was identified and functionally characterized in order to shed more light on the mechanisms underlying fungicide resistance. PdMFS1 can play an important role in the intensification of resistance to fungicides normally used in P. digitatum postharvest treatments. In the PdMFS1 disrupted mutants, a slight effect in response to chemical fungicides was observed, but fungicide sensitivity was highly affected in the overexpression mutants which became resistant to wide range of chemical fungicides. Moreover, P. digitatum knock-out mutants exhibited a lower rate of fungal virulence when infected oranges were stored at 20 degrees C. Disease symptoms were higher in the PdMFS1 overexpression mutants coming from the low-virulent P. digitatum parental strain. In addition, the gene expression analysis showed an induction of PdMFS1 transcription in all overexpression mutants regardless from which progenitor came from, and four-time intensification of the parental wild type strain during citrus infection reinforcing PdMFS1 role in fungal virulence. The P. digitatum MFS transporter PdMFS1 contributes not only to the acquisition of wide range of fungicide resistance but also in fungal virulence during citrus infection. |
| format | article |
| id | ReDivia6300 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| record_format | dspace |
| spelling | ReDivia63002025-04-25T14:46:47Z PdMFS1 Transporter Contributes to Penicilliun digitatum Fungicide Resistance and Fungal Virulence during Citrus Fruit Infection De-Ramón-Carbonell, Marta López-Pérez, Mario Gonzalez-Candelas, Luis Sánchez-Torres, Paloma A new Penicillium digitatum major facilitator superfamily (MFS) transporter (PdMFS1) was identified and functionally characterized in order to shed more light on the mechanisms underlying fungicide resistance. PdMFS1 can play an important role in the intensification of resistance to fungicides normally used in P. digitatum postharvest treatments. In the PdMFS1 disrupted mutants, a slight effect in response to chemical fungicides was observed, but fungicide sensitivity was highly affected in the overexpression mutants which became resistant to wide range of chemical fungicides. Moreover, P. digitatum knock-out mutants exhibited a lower rate of fungal virulence when infected oranges were stored at 20 degrees C. Disease symptoms were higher in the PdMFS1 overexpression mutants coming from the low-virulent P. digitatum parental strain. In addition, the gene expression analysis showed an induction of PdMFS1 transcription in all overexpression mutants regardless from which progenitor came from, and four-time intensification of the parental wild type strain during citrus infection reinforcing PdMFS1 role in fungal virulence. The P. digitatum MFS transporter PdMFS1 contributes not only to the acquisition of wide range of fungicide resistance but also in fungal virulence during citrus infection. 2020-02-21T11:51:30Z 2020-02-21T11:51:30Z 2019 article publishedVersion de Ramon-Carbonell, M., Lopez-Perez, M., Gonzalez-Candelas, L., & Sanchez-Torres, P. (2019). PdMFS1 Transporter Contributes to Penicilliun digitatum Fungicide Resistance and Fungal Virulence during Citrus Fruit Infection. Journal of Fungi, 5(4). 2309-608X http://hdl.handle.net/20.500.11939/6300 10.3390/jof5040100 https://www.mdpi.com/2309-608X/5/4/100 en openAccess electronico |
| spellingShingle | De-Ramón-Carbonell, Marta López-Pérez, Mario Gonzalez-Candelas, Luis Sánchez-Torres, Paloma PdMFS1 Transporter Contributes to Penicilliun digitatum Fungicide Resistance and Fungal Virulence during Citrus Fruit Infection |
| title | PdMFS1 Transporter Contributes to Penicilliun digitatum Fungicide Resistance and Fungal Virulence during Citrus Fruit Infection |
| title_full | PdMFS1 Transporter Contributes to Penicilliun digitatum Fungicide Resistance and Fungal Virulence during Citrus Fruit Infection |
| title_fullStr | PdMFS1 Transporter Contributes to Penicilliun digitatum Fungicide Resistance and Fungal Virulence during Citrus Fruit Infection |
| title_full_unstemmed | PdMFS1 Transporter Contributes to Penicilliun digitatum Fungicide Resistance and Fungal Virulence during Citrus Fruit Infection |
| title_short | PdMFS1 Transporter Contributes to Penicilliun digitatum Fungicide Resistance and Fungal Virulence during Citrus Fruit Infection |
| title_sort | pdmfs1 transporter contributes to penicilliun digitatum fungicide resistance and fungal virulence during citrus fruit infection |
| url | http://hdl.handle.net/20.500.11939/6300 https://www.mdpi.com/2309-608X/5/4/100 |
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