Molecular Characterization and Stress Tolerance Evaluation of New Allotetraploid Somatic Hybrids Between Carrizo Citrange and Citrus macrophylla W. rootstocks
Polyploidy is one of the main forces that drives the evolution of plants and provides great advantages for breeding. Somatic hybridization by protoplast fusion is used in citrus breeding programs. This method allows combining the whole parental genomes in a single genotype, adding complementary d...
| Autores principales: | , , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6256 https://www.frontiersin.org/articles/10.3389/fpls.2018.00901/full |
| _version_ | 1855032394586783744 |
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| author | Ruiz, Marta Pensabene-Bellavia, Giovanni Quinones, Ana García-Lor, Andrés Morillon, Raphael Ollitrault, Patrick Primo-Millo, Eduardo Navarro, Luis Aleza, Pablo |
| author_browse | Aleza, Pablo García-Lor, Andrés Morillon, Raphael Navarro, Luis Ollitrault, Patrick Pensabene-Bellavia, Giovanni Primo-Millo, Eduardo Quinones, Ana Ruiz, Marta |
| author_facet | Ruiz, Marta Pensabene-Bellavia, Giovanni Quinones, Ana García-Lor, Andrés Morillon, Raphael Ollitrault, Patrick Primo-Millo, Eduardo Navarro, Luis Aleza, Pablo |
| author_sort | Ruiz, Marta |
| collection | ReDivia |
| description | Polyploidy is one of the main forces that drives the evolution of plants and provides
great advantages for breeding. Somatic hybridization by protoplast fusion is used in
citrus breeding programs. This method allows combining the whole parental genomes in
a single genotype, adding complementary dominant characters, regardless of parental
heterozygosity. It also contributes to surpass limitations imposed by reproductive biology
and quickly generates progenies that combine the required traits. Two allotetraploid
somatic hybrids recovered from the citrus rootstocks—Citrus macrophylla (CM) and
Carrizo citrange (CC)—were characterized for morphology, genome composition using
molecular markers (SNP, SSR, and InDel), and their tolerance to iron chlorosis, salinity,
and Citrus tristeza virus (CTV). Both hybrids combine the whole parental genomes even
though the loss of parental alleles was detected in most linkage groups. Mitochondrial
genome was inherited from CM in both the hybrids, whereas recombination was
observed for chloroplastic genome. Thus, somatic hybrids differ from each other in their
genome composition, indicating that losses and rearrangements occurred during the
fusion process. Both inherited the tolerance to stem pitting caused by CTV from CC,
are tolerant to iron chlorosis such as CM, and have a higher tolerance to salinity than
the sensitive CC. These hybrids have potential as improved rootstocks to grow citrus in
areas with calcareous and saline soils where CTV is present, such as the Mediterranean
region. The provided knowledge on the effects of somatic hybridization on the genome
composition, anatomy, and physiology of citrus rootstocks will be key for breeding
programs that aim to address current and future needs of the citrus industry. |
| format | article |
| id | ReDivia6256 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| record_format | dspace |
| spelling | ReDivia62562025-04-25T14:46:40Z Molecular Characterization and Stress Tolerance Evaluation of New Allotetraploid Somatic Hybrids Between Carrizo Citrange and Citrus macrophylla W. rootstocks Ruiz, Marta Pensabene-Bellavia, Giovanni Quinones, Ana García-Lor, Andrés Morillon, Raphael Ollitrault, Patrick Primo-Millo, Eduardo Navarro, Luis Aleza, Pablo Rootstock breeding Protoplast fusion Genome instability Tetraploid Polyploid F30 Plant genetics and breeding Polyploidy is one of the main forces that drives the evolution of plants and provides great advantages for breeding. Somatic hybridization by protoplast fusion is used in citrus breeding programs. This method allows combining the whole parental genomes in a single genotype, adding complementary dominant characters, regardless of parental heterozygosity. It also contributes to surpass limitations imposed by reproductive biology and quickly generates progenies that combine the required traits. Two allotetraploid somatic hybrids recovered from the citrus rootstocks—Citrus macrophylla (CM) and Carrizo citrange (CC)—were characterized for morphology, genome composition using molecular markers (SNP, SSR, and InDel), and their tolerance to iron chlorosis, salinity, and Citrus tristeza virus (CTV). Both hybrids combine the whole parental genomes even though the loss of parental alleles was detected in most linkage groups. Mitochondrial genome was inherited from CM in both the hybrids, whereas recombination was observed for chloroplastic genome. Thus, somatic hybrids differ from each other in their genome composition, indicating that losses and rearrangements occurred during the fusion process. Both inherited the tolerance to stem pitting caused by CTV from CC, are tolerant to iron chlorosis such as CM, and have a higher tolerance to salinity than the sensitive CC. These hybrids have potential as improved rootstocks to grow citrus in areas with calcareous and saline soils where CTV is present, such as the Mediterranean region. The provided knowledge on the effects of somatic hybridization on the genome composition, anatomy, and physiology of citrus rootstocks will be key for breeding programs that aim to address current and future needs of the citrus industry. 2019-06-07T07:57:19Z 2019-06-07T07:57:19Z 2018 article publishedVersion Ruiz M, Pensabene-Bellavia G, Quiñones A, García-Lor A, Morillon R, Ollitrault P, Primo-Millo E, Navarro L and Aleza P (2018) Molecular Characterization and Stress Tolerance Evaluation of New Allotetraploid Somatic Hybrids Between Carrizo Citrange and Citrus macrophylla W. rootstocks. Front. Plant Sci. 9:901. http://hdl.handle.net/20.500.11939/6256 10.3389/fpls.2018.00901 https://www.frontiersin.org/articles/10.3389/fpls.2018.00901/full en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ electronico |
| spellingShingle | Rootstock breeding Protoplast fusion Genome instability Tetraploid Polyploid F30 Plant genetics and breeding Ruiz, Marta Pensabene-Bellavia, Giovanni Quinones, Ana García-Lor, Andrés Morillon, Raphael Ollitrault, Patrick Primo-Millo, Eduardo Navarro, Luis Aleza, Pablo Molecular Characterization and Stress Tolerance Evaluation of New Allotetraploid Somatic Hybrids Between Carrizo Citrange and Citrus macrophylla W. rootstocks |
| title | Molecular Characterization and Stress Tolerance Evaluation of New Allotetraploid Somatic Hybrids Between Carrizo Citrange and Citrus macrophylla W. rootstocks |
| title_full | Molecular Characterization and Stress Tolerance Evaluation of New Allotetraploid Somatic Hybrids Between Carrizo Citrange and Citrus macrophylla W. rootstocks |
| title_fullStr | Molecular Characterization and Stress Tolerance Evaluation of New Allotetraploid Somatic Hybrids Between Carrizo Citrange and Citrus macrophylla W. rootstocks |
| title_full_unstemmed | Molecular Characterization and Stress Tolerance Evaluation of New Allotetraploid Somatic Hybrids Between Carrizo Citrange and Citrus macrophylla W. rootstocks |
| title_short | Molecular Characterization and Stress Tolerance Evaluation of New Allotetraploid Somatic Hybrids Between Carrizo Citrange and Citrus macrophylla W. rootstocks |
| title_sort | molecular characterization and stress tolerance evaluation of new allotetraploid somatic hybrids between carrizo citrange and citrus macrophylla w rootstocks |
| topic | Rootstock breeding Protoplast fusion Genome instability Tetraploid Polyploid F30 Plant genetics and breeding |
| url | http://hdl.handle.net/20.500.11939/6256 https://www.frontiersin.org/articles/10.3389/fpls.2018.00901/full |
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