Transcriptional response of Erwinia amylovora to copper shock: in vivo role of the copA gene
Fire blight is a devastating plant disease caused by the bacterium Erwinia amylovora, and its control is frequently based on the use of copper‐based compounds whose mechanisms of action are not well known. Consequently, in this article, we investigate the response of E. amylovora to copper shock by...
| Main Authors: | , , , , , , , |
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| Format: | article |
| Language: | Inglés |
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John Wiley & Sons
2019
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11939/6253 https://onlinelibrary.wiley.com/doi/full/10.1111/mpp.12510 |
| _version_ | 1855032394057252864 |
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| author | Águila-Clarés, Begoña Castiblanco, Luisa F. Quesada, Jose M. Penyalver, Ramón Carbonell, Juan López, María M. Marco-Noales, Ester Sundin, Georges W. |
| author_browse | Carbonell, Juan Castiblanco, Luisa F. López, María M. Marco-Noales, Ester Penyalver, Ramón Quesada, Jose M. Sundin, Georges W. Águila-Clarés, Begoña |
| author_facet | Águila-Clarés, Begoña Castiblanco, Luisa F. Quesada, Jose M. Penyalver, Ramón Carbonell, Juan López, María M. Marco-Noales, Ester Sundin, Georges W. |
| author_sort | Águila-Clarés, Begoña |
| collection | ReDivia |
| description | Fire blight is a devastating plant disease caused by the bacterium Erwinia amylovora, and its control is frequently based on the use of copper‐based compounds whose mechanisms of action are not well known. Consequently, in this article, we investigate the response of E. amylovora to copper shock by a whole‐genome microarray approach. Transcriptional analyses showed that, in the presence of copper, 23 genes were increased in expression; these genes were classified mainly into the transport and stress functional categories. Among them, the copA gene was strongly induced and regulated in a finely tuned manner by copper. Mutation of copA, soxS, arcB, yjcE, ygcF, yhhQ, galF and EAM_3469 genes revealed that tolerance to copper in E. amylovora can be achieved by complex physiological mechanisms, including: (i) the control of copper homeostasis through, at least, the extrusion of Cu(I) by a P‐type ATPase efflux pump CopA; and (ii) the overcoming of copper toxicity caused by oxidative stress by the expression of several reactive oxygen species (ROS)‐related genes, including the two major transcriptional factors SoxS and ArcB. Furthermore, complementation analyses demonstrated the important role of copA for copper tolerance in E. amylovora, not only in vitro, but also in inoculated pear shoots. |
| format | article |
| id | ReDivia6253 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | John Wiley & Sons |
| publisherStr | John Wiley & Sons |
| record_format | dspace |
| spelling | ReDivia62532025-04-25T14:46:40Z Transcriptional response of Erwinia amylovora to copper shock: in vivo role of the copA gene Águila-Clarés, Begoña Castiblanco, Luisa F. Quesada, Jose M. Penyalver, Ramón Carbonell, Juan López, María M. Marco-Noales, Ester Sundin, Georges W. Copper homeostasis copA H20 Plant diseases Fire blight Fire blight is a devastating plant disease caused by the bacterium Erwinia amylovora, and its control is frequently based on the use of copper‐based compounds whose mechanisms of action are not well known. Consequently, in this article, we investigate the response of E. amylovora to copper shock by a whole‐genome microarray approach. Transcriptional analyses showed that, in the presence of copper, 23 genes were increased in expression; these genes were classified mainly into the transport and stress functional categories. Among them, the copA gene was strongly induced and regulated in a finely tuned manner by copper. Mutation of copA, soxS, arcB, yjcE, ygcF, yhhQ, galF and EAM_3469 genes revealed that tolerance to copper in E. amylovora can be achieved by complex physiological mechanisms, including: (i) the control of copper homeostasis through, at least, the extrusion of Cu(I) by a P‐type ATPase efflux pump CopA; and (ii) the overcoming of copper toxicity caused by oxidative stress by the expression of several reactive oxygen species (ROS)‐related genes, including the two major transcriptional factors SoxS and ArcB. Furthermore, complementation analyses demonstrated the important role of copA for copper tolerance in E. amylovora, not only in vitro, but also in inoculated pear shoots. 2019-05-30T11:20:03Z 2019-05-30T11:20:03Z 2018 article Águila‐Clares, B., Castiblanco, L. F., Quesada, J. M., Penyalver, R., Carbonell, J., López, M. M.et al. (2018). Transcriptional response of Erwinia amylovora to copper shock: in vivo role of the copA gene. Molecular plant pathology, 19(1), 169-179. http://hdl.handle.net/20.500.11939/6253 10.1111/mpp.12510 https://onlinelibrary.wiley.com/doi/full/10.1111/mpp.12510 en_US Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ John Wiley & Sons electronico |
| spellingShingle | Copper homeostasis copA H20 Plant diseases Fire blight Águila-Clarés, Begoña Castiblanco, Luisa F. Quesada, Jose M. Penyalver, Ramón Carbonell, Juan López, María M. Marco-Noales, Ester Sundin, Georges W. Transcriptional response of Erwinia amylovora to copper shock: in vivo role of the copA gene |
| title | Transcriptional response of Erwinia amylovora to copper shock: in vivo role of the copA gene |
| title_full | Transcriptional response of Erwinia amylovora to copper shock: in vivo role of the copA gene |
| title_fullStr | Transcriptional response of Erwinia amylovora to copper shock: in vivo role of the copA gene |
| title_full_unstemmed | Transcriptional response of Erwinia amylovora to copper shock: in vivo role of the copA gene |
| title_short | Transcriptional response of Erwinia amylovora to copper shock: in vivo role of the copA gene |
| title_sort | transcriptional response of erwinia amylovora to copper shock in vivo role of the copa gene |
| topic | Copper homeostasis copA H20 Plant diseases Fire blight |
| url | http://hdl.handle.net/20.500.11939/6253 https://onlinelibrary.wiley.com/doi/full/10.1111/mpp.12510 |
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