Unravelling the contribution of the Penicillium expansum PeSte12 transcription factor to virulence during apple fruit infection
Blue mould disease caused by Penicillium expansum infection is one of the most important diseases of pome fruit accounting for important economic losses. In the present study, the PeSte12 transcription factor gene was identified, and deletant mutants were produced by gene replacement. Knockout mutan...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2019
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/6241 |
| _version_ | 1855492044229705728 |
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| author | Sánchez-Torres, Paloma Vilanova, Laura Ballester, Ana Rosa López-Pérez, Mario Teixidó, Neus Vinas, Inmaculada Usall, Josep Gonzalez-Candelas, Luis Torres, Rosario |
| author_browse | Ballester, Ana Rosa Gonzalez-Candelas, Luis López-Pérez, Mario Sánchez-Torres, Paloma Teixidó, Neus Torres, Rosario Usall, Josep Vilanova, Laura Vinas, Inmaculada |
| author_facet | Sánchez-Torres, Paloma Vilanova, Laura Ballester, Ana Rosa López-Pérez, Mario Teixidó, Neus Vinas, Inmaculada Usall, Josep Gonzalez-Candelas, Luis Torres, Rosario |
| author_sort | Sánchez-Torres, Paloma |
| collection | ReDivia |
| description | Blue mould disease caused by Penicillium expansum infection is one of the most important diseases of pome fruit accounting for important economic losses. In the present study, the PeSte12 transcription factor gene was identified, and deletant mutants were produced by gene replacement. Knockout mutants showed a significant decrease of virulence during apple fruit infection. Virulence was affected by the maturity stage of the fruit (immature, mature and over-mature), and disease severity was notably reduced when the apples were stored at 0 degrees C. The Delta PeSte12 mutants resulted defective in asexual reproduction, producing less conidia, but this characteristic did not correlate with differences in microscopic morphology. In addition, the DPeSte12 mutants produced higher quantity of hydrogen peroxide than the wild type strain. Gene expression analysis revealed that PeSte12 was induced over time during apple infection compared to axenic growth, particularly from 2 dpi, reinforcing its role in virulence. Analysis of transcriptional abundance of several genes in Delta PeSte12 mutants showed that in most of the evaluated genes, PeSte12 seemed to act as a negative regulator during axenic growth, as most of them exhibited an increasing expression pattern along the time period evaluated. The highest expression values corresponded to detoxification, ATPase activity, protein folding and basic metabolism. Gene expression analysis during apple infection showed that 3 out of 9 analysed genes were up regulated; thus, PeSte12 seemed to exert a positive control to particular type of aldolase. These results demonstrate the PeSte12 transcription factor could play an important role in P. expansum's virulence and asexual reproduction. (C) 2017 Elsevier Ltd. All rights reserved. |
| format | Artículo |
| id | ReDivia6241 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| record_format | dspace |
| spelling | ReDivia62412025-04-25T14:46:37Z Unravelling the contribution of the Penicillium expansum PeSte12 transcription factor to virulence during apple fruit infection Sánchez-Torres, Paloma Vilanova, Laura Ballester, Ana Rosa López-Pérez, Mario Teixidó, Neus Vinas, Inmaculada Usall, Josep Gonzalez-Candelas, Luis Torres, Rosario Blue mould disease caused by Penicillium expansum infection is one of the most important diseases of pome fruit accounting for important economic losses. In the present study, the PeSte12 transcription factor gene was identified, and deletant mutants were produced by gene replacement. Knockout mutants showed a significant decrease of virulence during apple fruit infection. Virulence was affected by the maturity stage of the fruit (immature, mature and over-mature), and disease severity was notably reduced when the apples were stored at 0 degrees C. The Delta PeSte12 mutants resulted defective in asexual reproduction, producing less conidia, but this characteristic did not correlate with differences in microscopic morphology. In addition, the DPeSte12 mutants produced higher quantity of hydrogen peroxide than the wild type strain. Gene expression analysis revealed that PeSte12 was induced over time during apple infection compared to axenic growth, particularly from 2 dpi, reinforcing its role in virulence. Analysis of transcriptional abundance of several genes in Delta PeSte12 mutants showed that in most of the evaluated genes, PeSte12 seemed to act as a negative regulator during axenic growth, as most of them exhibited an increasing expression pattern along the time period evaluated. The highest expression values corresponded to detoxification, ATPase activity, protein folding and basic metabolism. Gene expression analysis during apple infection showed that 3 out of 9 analysed genes were up regulated; thus, PeSte12 seemed to exert a positive control to particular type of aldolase. These results demonstrate the PeSte12 transcription factor could play an important role in P. expansum's virulence and asexual reproduction. (C) 2017 Elsevier Ltd. All rights reserved. 2019-05-15T10:37:43Z 2019-05-15T10:37:43Z 2018 article acceptedVersion Sánchez-Torres, P.; Vilanova, L.; Rosa Ballester, A.; López-Pérez, M.; Teixido, N.; Vinas, I.; Usall, J.; Gonzalez-Candelas, L.; Torres, R. (2018). Unravelling the contribution of the penicillium expansum PeSte12 transcription factor to virulence during apple fruit infection. Food Microbiology, 69, 123-135. http://hdl.handle.net/20.500.11939/6241 10.1016/j.fm.2017.08.005 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ electronico |
| spellingShingle | Sánchez-Torres, Paloma Vilanova, Laura Ballester, Ana Rosa López-Pérez, Mario Teixidó, Neus Vinas, Inmaculada Usall, Josep Gonzalez-Candelas, Luis Torres, Rosario Unravelling the contribution of the Penicillium expansum PeSte12 transcription factor to virulence during apple fruit infection |
| title | Unravelling the contribution of the Penicillium expansum PeSte12 transcription factor to virulence during apple fruit infection |
| title_full | Unravelling the contribution of the Penicillium expansum PeSte12 transcription factor to virulence during apple fruit infection |
| title_fullStr | Unravelling the contribution of the Penicillium expansum PeSte12 transcription factor to virulence during apple fruit infection |
| title_full_unstemmed | Unravelling the contribution of the Penicillium expansum PeSte12 transcription factor to virulence during apple fruit infection |
| title_short | Unravelling the contribution of the Penicillium expansum PeSte12 transcription factor to virulence during apple fruit infection |
| title_sort | unravelling the contribution of the penicillium expansum peste12 transcription factor to virulence during apple fruit infection |
| url | http://hdl.handle.net/20.500.11939/6241 |
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