Evaluation of conditions for in vitro storage of commercial and minor grapevine (Vitis vinifera L.) cultivars
In vitro culture represents a tool for the ex situ conservation of a high number of sanitised plants in a reduced space. However, the culture media and/or other growing conditions need to be optimised to minimising plant growth and storage cost. Growth on MW medium was evaluated in the commercial cu...
| Main Authors: | , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
2019
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| Online Access: | http://hdl.handle.net/20.500.11939/6240 https://www.tandfonline.com/doi/abs/10.1080/14620316.2017.1352462?journalCode=thsb20 |
| _version_ | 1855492044036767744 |
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| author | San Pedro, Tania Peiró, Rosa Jiménez, Carles Olmos, Antonio Gisbert, Carmina |
| author_browse | Gisbert, Carmina Jiménez, Carles Olmos, Antonio Peiró, Rosa San Pedro, Tania |
| author_facet | San Pedro, Tania Peiró, Rosa Jiménez, Carles Olmos, Antonio Gisbert, Carmina |
| author_sort | San Pedro, Tania |
| collection | ReDivia |
| description | In vitro culture represents a tool for the ex situ conservation of a high number of sanitised plants in a reduced space. However, the culture media and/or other growing conditions need to be optimised to minimising plant growth and storage cost. Growth on MW medium was evaluated in the commercial cultivars 'Airen', 'Bobal', 'Chardonnay', 'Garnacha Blanca', 'Moscatel de Alejandria', 'Moscatel de Grano Menudo', 'Pedro Ximenez', 'Pinot Blanc', 'Pinot Gris', 'Sauvignon Blanc', and 'Tempranillo'; the minor cultivars 'Chelva', 'Valenci Negre', 'Valenci Blanc', and 'Verdil'; and the endangered cv. 'Esclafacherre'. Different growth rates were observed among cultivars: those with faster growth need to be subcultured every 1.5-2.0 months; those with the slowest growth every 3.5-4.0 months. The effect of halving the sucrose in MW reduced the growth of the cultivars that grew faster without compromising survival. When IBA was removed from MW, growth was also reduced in some cultivars. Therefore, small modifications of the MW composition are adequate for grapevine in vitro storage under standard incubation conditions. This is an advantage with respect to the change of temperature used in other work to achieve growth reduction, and allows the use of the same chamber for different in vitro culture procedures. |
| format | Artículo |
| id | ReDivia6240 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| record_format | dspace |
| spelling | ReDivia62402025-04-25T14:46:37Z Evaluation of conditions for in vitro storage of commercial and minor grapevine (Vitis vinifera L.) cultivars San Pedro, Tania Peiró, Rosa Jiménez, Carles Olmos, Antonio Gisbert, Carmina In vitro culture represents a tool for the ex situ conservation of a high number of sanitised plants in a reduced space. However, the culture media and/or other growing conditions need to be optimised to minimising plant growth and storage cost. Growth on MW medium was evaluated in the commercial cultivars 'Airen', 'Bobal', 'Chardonnay', 'Garnacha Blanca', 'Moscatel de Alejandria', 'Moscatel de Grano Menudo', 'Pedro Ximenez', 'Pinot Blanc', 'Pinot Gris', 'Sauvignon Blanc', and 'Tempranillo'; the minor cultivars 'Chelva', 'Valenci Negre', 'Valenci Blanc', and 'Verdil'; and the endangered cv. 'Esclafacherre'. Different growth rates were observed among cultivars: those with faster growth need to be subcultured every 1.5-2.0 months; those with the slowest growth every 3.5-4.0 months. The effect of halving the sucrose in MW reduced the growth of the cultivars that grew faster without compromising survival. When IBA was removed from MW, growth was also reduced in some cultivars. Therefore, small modifications of the MW composition are adequate for grapevine in vitro storage under standard incubation conditions. This is an advantage with respect to the change of temperature used in other work to achieve growth reduction, and allows the use of the same chamber for different in vitro culture procedures. 2019-05-15T10:37:43Z 2019-05-15T10:37:43Z 2018 article San Pedro, T.; Peiro, R.; Jimenez, C.; Olmos, A.; Gisbert, C. (2018). Evaluation of conditions for in vitro storage of commercial and minor grapevine (vitis vinifera L.) cultivars. Journal of Horticultural Science & Biotechnology, 93(1), 19-25. http://hdl.handle.net/20.500.11939/6240 10.1080/14620316.2017.1352462 https://www.tandfonline.com/doi/abs/10.1080/14620316.2017.1352462?journalCode=thsb20 en closedAccess electronico |
| spellingShingle | San Pedro, Tania Peiró, Rosa Jiménez, Carles Olmos, Antonio Gisbert, Carmina Evaluation of conditions for in vitro storage of commercial and minor grapevine (Vitis vinifera L.) cultivars |
| title | Evaluation of conditions for in vitro storage of commercial and minor grapevine (Vitis vinifera L.) cultivars |
| title_full | Evaluation of conditions for in vitro storage of commercial and minor grapevine (Vitis vinifera L.) cultivars |
| title_fullStr | Evaluation of conditions for in vitro storage of commercial and minor grapevine (Vitis vinifera L.) cultivars |
| title_full_unstemmed | Evaluation of conditions for in vitro storage of commercial and minor grapevine (Vitis vinifera L.) cultivars |
| title_short | Evaluation of conditions for in vitro storage of commercial and minor grapevine (Vitis vinifera L.) cultivars |
| title_sort | evaluation of conditions for in vitro storage of commercial and minor grapevine vitis vinifera l cultivars |
| url | http://hdl.handle.net/20.500.11939/6240 https://www.tandfonline.com/doi/abs/10.1080/14620316.2017.1352462?journalCode=thsb20 |
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