Reliable detection of Peach latent mosaic viroid (PLMVd) by real-time RT-PCR
| Main Authors: | , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
2019
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| Online Access: | http://hdl.handle.net/20.500.11939/6225 https://apsjournals.apsnet.org/doi/pdfplus/10.1094/PHYTO-108-10-S1.1 |
| _version_ | 1855492041316761600 |
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| author | Martínez, M. Carmen Serra, P. Bertolini, Edson Cambra, Mariano Flores, R. |
| author_browse | Bertolini, Edson Cambra, Mariano Flores, R. Martínez, M. Carmen Serra, P. |
| author_facet | Martínez, M. Carmen Serra, P. Bertolini, Edson Cambra, Mariano Flores, R. |
| author_sort | Martínez, M. Carmen |
| collection | ReDivia |
| description | |
| format | Artículo |
| id | ReDivia6225 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| record_format | dspace |
| spelling | ReDivia62252025-04-25T14:46:34Z Reliable detection of Peach latent mosaic viroid (PLMVd) by real-time RT-PCR Martínez, M. Carmen Serra, P. Bertolini, Edson Cambra, Mariano Flores, R. Peach latent mosaic viroid (PLMVd) is a worldwide-distributed pathogen that causes leaf mosaics, delays in foliation, flowering and ripening, irregularly-shaped and discolored fruits with cracked sutures and, in a few cases, albinism (peach calico). PLMVd variants are clustered into two different classes, I and II, with the latter presenting multiple changes with respect to the former in the region delimited by positions 161-197 (stems p6a and p6b). We have developed a real-time RT-PCR method able to detect PLMVd variants. Three different TaqMan probes were designed: P1 and P2 specific for class I and II variants, and P3 for all PLMVd variants deposited in databases. Our real-time RT-PCR approach has been used to characterize PLMVd populations present in the peach indicator GF305 after independent inoculation of representative variants of class I and II, and co-inoculation with both variants. Plants were analyzed by hybridization with a full-length PLMVd riboprobe, sequencing of conventional RT-PCR products, and realtime RT-PCR. Under greenhouse conditions the results showed a strong competition between both variants during host colonization, since only class II variants were detected in the co-inoculated plants. These results point to a higher biological fitness of class II PLMVd variants. 2019-05-15T10:37:42Z 2019-05-15T10:37:42Z 2018 article Martínez, C.; Serra, P.; Bertolini, E.; Cambra, M. J.; Flores, R. (2018). Reliable detection of peach latent mosaic viroid (PLMVd) by real-time RT-PCR. Phytopathology, 108(10), 60. http://hdl.handle.net/20.500.11939/6225 10.1094/PHYTO-108-10-S1.1 https://apsjournals.apsnet.org/doi/pdfplus/10.1094/PHYTO-108-10-S1.1 en closedAccess electronico |
| spellingShingle | Martínez, M. Carmen Serra, P. Bertolini, Edson Cambra, Mariano Flores, R. Reliable detection of Peach latent mosaic viroid (PLMVd) by real-time RT-PCR |
| title | Reliable detection of Peach latent mosaic viroid (PLMVd) by real-time RT-PCR |
| title_full | Reliable detection of Peach latent mosaic viroid (PLMVd) by real-time RT-PCR |
| title_fullStr | Reliable detection of Peach latent mosaic viroid (PLMVd) by real-time RT-PCR |
| title_full_unstemmed | Reliable detection of Peach latent mosaic viroid (PLMVd) by real-time RT-PCR |
| title_short | Reliable detection of Peach latent mosaic viroid (PLMVd) by real-time RT-PCR |
| title_sort | reliable detection of peach latent mosaic viroid plmvd by real time rt pcr |
| url | http://hdl.handle.net/20.500.11939/6225 https://apsjournals.apsnet.org/doi/pdfplus/10.1094/PHYTO-108-10-S1.1 |
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