Recent Advances on Detection and Characterization of Fruit Tree Viruses Using High-Throughput Sequencing Technologies
Perennial crops, such as fruit trees, are infected by many viruses, which are transmitted through vegetative propagation and grafting of infected plant material. Some of these pathogens cause severe crop losses and often reduce the productive life of the orchards. Detection and characterization of t...
| Main Authors: | , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
2019
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| Online Access: | http://hdl.handle.net/20.500.11939/6223 |
| _version_ | 1855492040945565696 |
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| author | Maliogka, Varvara Minafra, Angelantonio Saldarelli, Pasquale Ruiz-García, Ana B. Glasa, Miroslav Katis, Nikolaos Olmos, Antonio |
| author_browse | Glasa, Miroslav Katis, Nikolaos Maliogka, Varvara Minafra, Angelantonio Olmos, Antonio Ruiz-García, Ana B. Saldarelli, Pasquale |
| author_facet | Maliogka, Varvara Minafra, Angelantonio Saldarelli, Pasquale Ruiz-García, Ana B. Glasa, Miroslav Katis, Nikolaos Olmos, Antonio |
| author_sort | Maliogka, Varvara |
| collection | ReDivia |
| description | Perennial crops, such as fruit trees, are infected by many viruses, which are transmitted through vegetative propagation and grafting of infected plant material. Some of these pathogens cause severe crop losses and often reduce the productive life of the orchards. Detection and characterization of these agents in fruit trees is challenging, however, during the last years, the wide application of high-throughput sequencing (HTS) technologies has significantly facilitated this task. In this review, we present recent advances in the discovery, detection, and characterization of fruit tree viruses and virus-like agents accomplished by HTS approaches. A high number of new viruses have been described in the last 5 years, some of them exhibiting novel genomic features that have led to the proposal of the creation of new genera, and the revision of the current virus taxonomy status. Interestingly, several of the newly identified viruses belong to virus genera previously unknown to infect fruit tree species (e.g., Fabavirus, Luteovirus) a fact that challenges our perspective of plant viruses in general. Finally, applied methodologies, including the use of different molecules as templates, as well as advantages and disadvantages and future directions of HTS in fruit tree virology are discussed. |
| format | Artículo |
| id | ReDivia6223 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| record_format | dspace |
| spelling | ReDivia62232025-04-25T14:46:34Z Recent Advances on Detection and Characterization of Fruit Tree Viruses Using High-Throughput Sequencing Technologies Maliogka, Varvara Minafra, Angelantonio Saldarelli, Pasquale Ruiz-García, Ana B. Glasa, Miroslav Katis, Nikolaos Olmos, Antonio Perennial crops, such as fruit trees, are infected by many viruses, which are transmitted through vegetative propagation and grafting of infected plant material. Some of these pathogens cause severe crop losses and often reduce the productive life of the orchards. Detection and characterization of these agents in fruit trees is challenging, however, during the last years, the wide application of high-throughput sequencing (HTS) technologies has significantly facilitated this task. In this review, we present recent advances in the discovery, detection, and characterization of fruit tree viruses and virus-like agents accomplished by HTS approaches. A high number of new viruses have been described in the last 5 years, some of them exhibiting novel genomic features that have led to the proposal of the creation of new genera, and the revision of the current virus taxonomy status. Interestingly, several of the newly identified viruses belong to virus genera previously unknown to infect fruit tree species (e.g., Fabavirus, Luteovirus) a fact that challenges our perspective of plant viruses in general. Finally, applied methodologies, including the use of different molecules as templates, as well as advantages and disadvantages and future directions of HTS in fruit tree virology are discussed. 2019-05-15T10:37:42Z 2019-05-15T10:37:42Z 2018 article Maliogka, V.I.; Minafra, A.; Saldarelli, P.; Ruiz-Garcia, A. B.; Glasa, M.; Katis, N.; Olmos, A. (2018). Recent advances on detection and characterization of fruit tree viruses using high-throughput sequencing technologies. Viruses-Basel, 10(8), 436. http://hdl.handle.net/20.500.11939/6223 10.3390/v10080436 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ electronico |
| spellingShingle | Maliogka, Varvara Minafra, Angelantonio Saldarelli, Pasquale Ruiz-García, Ana B. Glasa, Miroslav Katis, Nikolaos Olmos, Antonio Recent Advances on Detection and Characterization of Fruit Tree Viruses Using High-Throughput Sequencing Technologies |
| title | Recent Advances on Detection and Characterization of Fruit Tree Viruses Using High-Throughput Sequencing Technologies |
| title_full | Recent Advances on Detection and Characterization of Fruit Tree Viruses Using High-Throughput Sequencing Technologies |
| title_fullStr | Recent Advances on Detection and Characterization of Fruit Tree Viruses Using High-Throughput Sequencing Technologies |
| title_full_unstemmed | Recent Advances on Detection and Characterization of Fruit Tree Viruses Using High-Throughput Sequencing Technologies |
| title_short | Recent Advances on Detection and Characterization of Fruit Tree Viruses Using High-Throughput Sequencing Technologies |
| title_sort | recent advances on detection and characterization of fruit tree viruses using high throughput sequencing technologies |
| url | http://hdl.handle.net/20.500.11939/6223 |
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