High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics
Little cherry virus 1 (LChV1, Velarivirus, Closteroviridae) is a widespread pathogen of sweet or sour cherry and other Prunus species, which exhibits high genetic diversity and lacks a putative efficient transmission vector. Thus far, four distinct phylogenetic clusters of LChV1 have been described,...
| Main Authors: | , , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
2019
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| Online Access: | http://hdl.handle.net/20.500.11939/6220 |
| _version_ | 1855492040313274368 |
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| author | Katsiani, Asimina Maliogka, Varvara Katis, Nikolaos Svanella-Dumas, Laurence Olmos, Antonio Ruiz-García, Ana B. Marais, Armelle Faure, Chantal Theil, Sebastien Lotos, Leonidas Candresse, Thierry |
| author_browse | Candresse, Thierry Faure, Chantal Katis, Nikolaos Katsiani, Asimina Lotos, Leonidas Maliogka, Varvara Marais, Armelle Olmos, Antonio Ruiz-García, Ana B. Svanella-Dumas, Laurence Theil, Sebastien |
| author_facet | Katsiani, Asimina Maliogka, Varvara Katis, Nikolaos Svanella-Dumas, Laurence Olmos, Antonio Ruiz-García, Ana B. Marais, Armelle Faure, Chantal Theil, Sebastien Lotos, Leonidas Candresse, Thierry |
| author_sort | Katsiani, Asimina |
| collection | ReDivia |
| description | Little cherry virus 1 (LChV1, Velarivirus, Closteroviridae) is a widespread pathogen of sweet or sour cherry and other Prunus species, which exhibits high genetic diversity and lacks a putative efficient transmission vector. Thus far, four distinct phylogenetic clusters of LChV1 have been described, including isolates from different Prunus species. The recent application of high throughput sequencing (HTS) technologies in fruit tree virology has facilitated the acquisition of new viral genomes and the study of virus diversity. In the present work, several new LChV1 isolates from different countries were fully sequenced using different HTS approaches. Our results reveal the presence of further genetic diversity within the LChV1 species. Interestingly, mixed infections of the same sweet cherry tree with different LChV1 variants were identified for the first time. Taken together, the high intra-host and intra-species diversities of LChV1 might affect its pathogenicity and have clear implications for its accurate diagnostics. |
| format | Artículo |
| id | ReDivia6220 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| record_format | dspace |
| spelling | ReDivia62202025-04-25T14:46:33Z High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics Katsiani, Asimina Maliogka, Varvara Katis, Nikolaos Svanella-Dumas, Laurence Olmos, Antonio Ruiz-García, Ana B. Marais, Armelle Faure, Chantal Theil, Sebastien Lotos, Leonidas Candresse, Thierry Little cherry virus 1 (LChV1, Velarivirus, Closteroviridae) is a widespread pathogen of sweet or sour cherry and other Prunus species, which exhibits high genetic diversity and lacks a putative efficient transmission vector. Thus far, four distinct phylogenetic clusters of LChV1 have been described, including isolates from different Prunus species. The recent application of high throughput sequencing (HTS) technologies in fruit tree virology has facilitated the acquisition of new viral genomes and the study of virus diversity. In the present work, several new LChV1 isolates from different countries were fully sequenced using different HTS approaches. Our results reveal the presence of further genetic diversity within the LChV1 species. Interestingly, mixed infections of the same sweet cherry tree with different LChV1 variants were identified for the first time. Taken together, the high intra-host and intra-species diversities of LChV1 might affect its pathogenicity and have clear implications for its accurate diagnostics. 2019-05-15T10:37:42Z 2019-05-15T10:37:42Z 2018 article Katsiani, A.; Maliogka, V. I.; Katis, N.; Svanella-Dumas, L.; Olmos, A.; Ruiz-Garcia, A. B.; Marais, A.; Faure, C.; Theil, S.; Lotos, L.; Candresse, T. (2018). High-throughput sequencing reveals further diversity of little cherry virus 1 with implications for diagnostics. Viruses-Basel, 10(7), 385. http://hdl.handle.net/20.500.11939/6220 10.3390/v10070385 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ electronico |
| spellingShingle | Katsiani, Asimina Maliogka, Varvara Katis, Nikolaos Svanella-Dumas, Laurence Olmos, Antonio Ruiz-García, Ana B. Marais, Armelle Faure, Chantal Theil, Sebastien Lotos, Leonidas Candresse, Thierry High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics |
| title | High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics |
| title_full | High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics |
| title_fullStr | High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics |
| title_full_unstemmed | High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics |
| title_short | High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics |
| title_sort | high throughput sequencing reveals further diversity of little cherry virus 1 with implications for diagnostics |
| url | http://hdl.handle.net/20.500.11939/6220 |
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