Fast detection by loop-mediated isothermal amplification (LAMP) of the three begomovirus species infecting tomato in Panama
Potato yellow mosaic Panama virus (PYMPV), Tomato leaf curl Sinaloa virus (ToLCSiV) and Tomato yellow mottle virus (TYMoV) of genus Begomovirus (family Geminiviridae) are the only three begomovirus species detected infecting tomato (Solanum lycopersicum L.) in Panama. PYMPV, ToLCSiV and TYMoV induce...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2019
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/6218 https://link.springer.com/article/10.1007%2Fs10658-017-1358-7 |
| _version_ | 1855492039924252672 |
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| author | Herrera-Vásquez, José A. Puchades, Andrés V. Elvira-González, Laura Jaén-Sanjur, José N. Carpino, Caterina Rubio, Luis Galipienso, Luis |
| author_browse | Carpino, Caterina Elvira-González, Laura Galipienso, Luis Herrera-Vásquez, José A. Jaén-Sanjur, José N. Puchades, Andrés V. Rubio, Luis |
| author_facet | Herrera-Vásquez, José A. Puchades, Andrés V. Elvira-González, Laura Jaén-Sanjur, José N. Carpino, Caterina Rubio, Luis Galipienso, Luis |
| author_sort | Herrera-Vásquez, José A. |
| collection | ReDivia |
| description | Potato yellow mosaic Panama virus (PYMPV), Tomato leaf curl Sinaloa virus (ToLCSiV) and Tomato yellow mottle virus (TYMoV) of genus Begomovirus (family Geminiviridae) are the only three begomovirus species detected infecting tomato (Solanum lycopersicum L.) in Panama. PYMPV, ToLCSiV and TYMoV induce symptoms of stunting, yellowing, curling, distortion of leaves and reduction of fruit size and cause important economic loses. A loop-mediated amplification under isothermal conditions (LAMP) assay was developed for the individual detection of these three begomovirus species by using a set of three primer pairs specific per each one of them. Amplification products were visualized by gel electrophoresis or direct Gel-Red staining of DNA into the reaction tube. PYMPV, ToLCSiV and TYMoV were detected in total DNA extracts obtained from different plant tissues such as leaves, stems, flowers, fruits and roots of infected tomato plants collected in different production regions of Panama. LAMP sensitivity was similar to that of conventional PCR but, the first procedure was faster and cheaper than the last one. Moreover, all three viruses were successfully detected by LAMP and not by conventional PCR from sap extracts obtained from leaf tissues of infected tomato plants which were embedded into 3MM Whatman paper and stored several days, facilitating the samples processing as well as the material movement among different laboratories. Therefore, LAMP is a specific, rapid and cheap procedure to detect all three begomoviruses infecting tomato in Panama and it is suitable for field surveys and sanitation programs. |
| format | Artículo |
| id | ReDivia6218 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| record_format | dspace |
| spelling | ReDivia62182025-04-25T14:46:32Z Fast detection by loop-mediated isothermal amplification (LAMP) of the three begomovirus species infecting tomato in Panama Herrera-Vásquez, José A. Puchades, Andrés V. Elvira-González, Laura Jaén-Sanjur, José N. Carpino, Caterina Rubio, Luis Galipienso, Luis Potato yellow mosaic Panama virus (PYMPV), Tomato leaf curl Sinaloa virus (ToLCSiV) and Tomato yellow mottle virus (TYMoV) of genus Begomovirus (family Geminiviridae) are the only three begomovirus species detected infecting tomato (Solanum lycopersicum L.) in Panama. PYMPV, ToLCSiV and TYMoV induce symptoms of stunting, yellowing, curling, distortion of leaves and reduction of fruit size and cause important economic loses. A loop-mediated amplification under isothermal conditions (LAMP) assay was developed for the individual detection of these three begomovirus species by using a set of three primer pairs specific per each one of them. Amplification products were visualized by gel electrophoresis or direct Gel-Red staining of DNA into the reaction tube. PYMPV, ToLCSiV and TYMoV were detected in total DNA extracts obtained from different plant tissues such as leaves, stems, flowers, fruits and roots of infected tomato plants collected in different production regions of Panama. LAMP sensitivity was similar to that of conventional PCR but, the first procedure was faster and cheaper than the last one. Moreover, all three viruses were successfully detected by LAMP and not by conventional PCR from sap extracts obtained from leaf tissues of infected tomato plants which were embedded into 3MM Whatman paper and stored several days, facilitating the samples processing as well as the material movement among different laboratories. Therefore, LAMP is a specific, rapid and cheap procedure to detect all three begomoviruses infecting tomato in Panama and it is suitable for field surveys and sanitation programs. 2019-05-15T10:37:42Z 2019-05-15T10:37:42Z 2018 article Herrera-Vasquez, J.A.; Puchades, A. V.; Elvira-Gonzalez, L.; Jaen-Sanjur, J. N.; Carpino, C.; Rubio, L.; Galipienso, L. (2018). Fast detection by loop-mediated isothermal amplification (LAMP) of the three begomovirus species infecting tomato in panama. European Journal of Plant Pathology, 151(1), 243-250. http://hdl.handle.net/20.500.11939/6218 10.1007/s10658-017-1358-7 https://link.springer.com/article/10.1007%2Fs10658-017-1358-7 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ electronico |
| spellingShingle | Herrera-Vásquez, José A. Puchades, Andrés V. Elvira-González, Laura Jaén-Sanjur, José N. Carpino, Caterina Rubio, Luis Galipienso, Luis Fast detection by loop-mediated isothermal amplification (LAMP) of the three begomovirus species infecting tomato in Panama |
| title | Fast detection by loop-mediated isothermal amplification (LAMP) of the three begomovirus species infecting tomato in Panama |
| title_full | Fast detection by loop-mediated isothermal amplification (LAMP) of the three begomovirus species infecting tomato in Panama |
| title_fullStr | Fast detection by loop-mediated isothermal amplification (LAMP) of the three begomovirus species infecting tomato in Panama |
| title_full_unstemmed | Fast detection by loop-mediated isothermal amplification (LAMP) of the three begomovirus species infecting tomato in Panama |
| title_short | Fast detection by loop-mediated isothermal amplification (LAMP) of the three begomovirus species infecting tomato in Panama |
| title_sort | fast detection by loop mediated isothermal amplification lamp of the three begomovirus species infecting tomato in panama |
| url | http://hdl.handle.net/20.500.11939/6218 https://link.springer.com/article/10.1007%2Fs10658-017-1358-7 |
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