Pepper Rootstock and Scion Physiological Responses Under Drought Stress
In vegetables, tolerance to drought can be improved by grafting commercial varieties onto drought tolerant rootstocks. Grafting has emerged as a tool that copes with drought stress. In previous results, the A25 pepper rootstock accession showed good tolerance to drought in fruit production terms com...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6162 https://www.frontiersin.org/articles/10.3389/fpls.2019.00038/full |
| _version_ | 1855492029487775744 |
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| author | López-Serrano, Lidia Canet-Sanchis, Guillermo Vuletin Selak, Gabriela Penella, Consuelo San Bautista, Alberto López-Galarza, Salvador Calatayud, Ángeles |
| author_browse | Calatayud, Ángeles Canet-Sanchis, Guillermo López-Galarza, Salvador López-Serrano, Lidia Penella, Consuelo San Bautista, Alberto Vuletin Selak, Gabriela |
| author_facet | López-Serrano, Lidia Canet-Sanchis, Guillermo Vuletin Selak, Gabriela Penella, Consuelo San Bautista, Alberto López-Galarza, Salvador Calatayud, Ángeles |
| author_sort | López-Serrano, Lidia |
| collection | ReDivia |
| description | In vegetables, tolerance to drought can be improved by grafting commercial varieties onto drought tolerant rootstocks. Grafting has emerged as a tool that copes with drought stress. In previous results, the A25 pepper rootstock accession showed good tolerance to drought in fruit production terms compared with non-grafted plants and other rootstocks. The aim of this work was to study if short-term exposure to drought in grafted plants using A25 as a rootstock would show tolerance to drought now. To fulfill this objective, some physiological processes involved in roots (rootstock) and leaves (scion) of grafted pepper plants were analyzed. Pepper plants not grafted (A), self-grafted (A/A), and grafted onto a tolerant pepper rootstock A25 (A/A25) were grown under severe water stress induced by PEG addition (-0.55 MPa) or under control conditions for 7 days in hydroponic pure solution. According to our results, water stress severity was alleviated by using the A25 rootstock in grafted plants (A/A25), which indicated that mechanisms stimulated by roots are essential to withstand stress. A/A25 had a bigger root biomass compared with plants A and A/A that resulted in better water absorption, water retention capacity and a sustained CO2 assimilation rate. Consequently, plants A/A25 had a better carbon balance, supported by greater nitrate reductase activity located mainly in leaves. In the non-grafted and self-grafted plants, the photosynthesis rate lowered due to stomatal closure, which limited transpiration. Consequently, part of NO3- uptake was reduced in roots. This condition limited water uptake and CO2 fixation in plants A and A/A under drought stress, and accelerated oxidative damage by producing reactive oxygen species (ROS) and H2O2, which were highest in their leaves, indicating great sensitivity to drought stress and induced membrane lipid peroxidation. However, drought deleterious effects were slightly marked in plants A compared to A/A. To conclude, the A25 rootstock protects the scion against oxidative stress, which is provoked by drought, and shows better C and N balances that enabled the biomass to be maintained under water stress for short-term exposure, with higher yields in the field. |
| format | Artículo |
| id | ReDivia6162 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| record_format | dspace |
| spelling | ReDivia61622025-04-25T14:46:22Z Pepper Rootstock and Scion Physiological Responses Under Drought Stress López-Serrano, Lidia Canet-Sanchis, Guillermo Vuletin Selak, Gabriela Penella, Consuelo San Bautista, Alberto López-Galarza, Salvador Calatayud, Ángeles Grafted F60 Plant physiology and biochemistry Drought tolerance Gas exchange Oxidative stress Pepper Rootstocks Plant water relations In vegetables, tolerance to drought can be improved by grafting commercial varieties onto drought tolerant rootstocks. Grafting has emerged as a tool that copes with drought stress. In previous results, the A25 pepper rootstock accession showed good tolerance to drought in fruit production terms compared with non-grafted plants and other rootstocks. The aim of this work was to study if short-term exposure to drought in grafted plants using A25 as a rootstock would show tolerance to drought now. To fulfill this objective, some physiological processes involved in roots (rootstock) and leaves (scion) of grafted pepper plants were analyzed. Pepper plants not grafted (A), self-grafted (A/A), and grafted onto a tolerant pepper rootstock A25 (A/A25) were grown under severe water stress induced by PEG addition (-0.55 MPa) or under control conditions for 7 days in hydroponic pure solution. According to our results, water stress severity was alleviated by using the A25 rootstock in grafted plants (A/A25), which indicated that mechanisms stimulated by roots are essential to withstand stress. A/A25 had a bigger root biomass compared with plants A and A/A that resulted in better water absorption, water retention capacity and a sustained CO2 assimilation rate. Consequently, plants A/A25 had a better carbon balance, supported by greater nitrate reductase activity located mainly in leaves. In the non-grafted and self-grafted plants, the photosynthesis rate lowered due to stomatal closure, which limited transpiration. Consequently, part of NO3- uptake was reduced in roots. This condition limited water uptake and CO2 fixation in plants A and A/A under drought stress, and accelerated oxidative damage by producing reactive oxygen species (ROS) and H2O2, which were highest in their leaves, indicating great sensitivity to drought stress and induced membrane lipid peroxidation. However, drought deleterious effects were slightly marked in plants A compared to A/A. To conclude, the A25 rootstock protects the scion against oxidative stress, which is provoked by drought, and shows better C and N balances that enabled the biomass to be maintained under water stress for short-term exposure, with higher yields in the field. 2019-02-11T11:38:30Z 2019-02-11T11:38:30Z 2019 article publishedVersion López-Serrano, L., Canet-Sanchis, G., Vuletin Selak, G., Penella, C., San Bautista, A., López-Galarza, S. and Calatayud, A. (2019). Pepper Rootstock and Scion Physiological Responses Under Drought Stress. Front. Plant Sci. 10:38, 1-13. http://hdl.handle.net/20.500.11939/6162 10.3389/fpls.2019.00038 https://www.frontiersin.org/articles/10.3389/fpls.2019.00038/full en electronico |
| spellingShingle | Grafted F60 Plant physiology and biochemistry Drought tolerance Gas exchange Oxidative stress Pepper Rootstocks Plant water relations López-Serrano, Lidia Canet-Sanchis, Guillermo Vuletin Selak, Gabriela Penella, Consuelo San Bautista, Alberto López-Galarza, Salvador Calatayud, Ángeles Pepper Rootstock and Scion Physiological Responses Under Drought Stress |
| title | Pepper Rootstock and Scion Physiological Responses Under Drought Stress |
| title_full | Pepper Rootstock and Scion Physiological Responses Under Drought Stress |
| title_fullStr | Pepper Rootstock and Scion Physiological Responses Under Drought Stress |
| title_full_unstemmed | Pepper Rootstock and Scion Physiological Responses Under Drought Stress |
| title_short | Pepper Rootstock and Scion Physiological Responses Under Drought Stress |
| title_sort | pepper rootstock and scion physiological responses under drought stress |
| topic | Grafted F60 Plant physiology and biochemistry Drought tolerance Gas exchange Oxidative stress Pepper Rootstocks Plant water relations |
| url | http://hdl.handle.net/20.500.11939/6162 https://www.frontiersin.org/articles/10.3389/fpls.2019.00038/full |
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