First Report of Grapevine Red Globe Virus in Grapevine in Germany
Grapevine red globe virus (GRGV) is a member of the genus Maculavirus, family Tymoviridae, found to infect grapevine for the first time in Italy (Sabanadzovic et al. 2000). GRGV has also been reported in Greece, the United States, France, China, and Spain (Cretazzo et al. 2017; Fan et al. 2016). No...
| Autores principales: | , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
The American Phytopathological Society
2019
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6154 https://apsjournals.apsnet.org/doi/10.1094/PDIS-01-18-0105-PDN |
| _version_ | 1855492027937980416 |
|---|---|
| author | Ruiz-García, Ana B. Nourinejhad Zarghani, Shaheen Okic, Arnela Olmos, Antonio Wetzel, Thierry |
| author_browse | Nourinejhad Zarghani, Shaheen Okic, Arnela Olmos, Antonio Ruiz-García, Ana B. Wetzel, Thierry |
| author_facet | Ruiz-García, Ana B. Nourinejhad Zarghani, Shaheen Okic, Arnela Olmos, Antonio Wetzel, Thierry |
| author_sort | Ruiz-García, Ana B. |
| collection | ReDivia |
| description | Grapevine red globe virus (GRGV) is a member of the genus Maculavirus, family Tymoviridae, found to infect grapevine for the first time in Italy (Sabanadzovic et al. 2000). GRGV has also been reported in Greece, the United States, France, China, and Spain (Cretazzo et al. 2017; Fan et al. 2016). No specific symptoms have been associated with GRGV infection (Martelli et al. 2002). In 2016, grapevine leaves from a Pinot Gris cultivar from the Rhineland-Palatinate area showing leaf mottling and flecking were collected and analyzed by high-throughput sequencing of siRNAs using TrueSeq Illumina technology on a HiSeq 1000 system. De novo assembly of the 5,658,588 reads was performed by Geneious software. The BlastN and BlastX analysis of the 316 contigs obtained revealed the presence of 65 contigs related to GRGV, with a percentage of nucleotide similarity ranging from 85.2 to 100%. Illumina reads were mapped to reference using the Graciano-T101 isolate (GenBank accession no. NC_030693.1), allowing the recovery of 90.1% of the viral sequence scattered along the genome. A partial sequence corresponding to a fragment of the GRGV replicase gene of one of the German isolates has been deposited in GenBank database (accession no. MG779496). Bioinformatic analysis of the remaining contigs showed the presence of Arabis mosaic virus, grapevine rupestris stem pitting associated virus, grapevine yellow speckle viroid-1, and hop stunt viroid. The presence of GRGV in the German vineyards was confirmed by reverse-transcription polymerase chain reaction (RT-PCR) using two different sets of primers, the generic primers for tymoviruses, 5′-CATGCANGTSAGRGGRCCRAA-3′ and 5′-CYCARCAYAARGTVAACGA-3′ (Kominek et al. 2009), and newly designed primers specific for GRGV, GRGV-CF-F1 5′-GAATTCGCTGTCGGCCACTC-3′ and GRGV-CF-R1 5′-AGTGAGAGGAGAGATTCCATC-3′. The expected RT-PCR products, of 453 and 386 bp respectively, were successfully amplified, cloned, and sequenced. BlastN analysis of all amplicons confirmed the presence of the virus. GRGV was also detected by RT-PCR in 14 out of 57 randomly collected samples from symptomatic and asymptomatic grapevines, in different grapevine growing areas from the Rhineland-Palatinate area. To our knowledge, this is the first report of GRGV in Germany. Further studies will be necessary to assess the relevance and potential threat of this virus to the grape production in Germany and to determine whether it should be regulated. |
| format | Artículo |
| id | ReDivia6154 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | The American Phytopathological Society |
| publisherStr | The American Phytopathological Society |
| record_format | dspace |
| spelling | ReDivia61542025-04-25T14:46:20Z First Report of Grapevine Red Globe Virus in Grapevine in Germany Ruiz-García, Ana B. Nourinejhad Zarghani, Shaheen Okic, Arnela Olmos, Antonio Wetzel, Thierry Grapevine red globe virus NGS Illumina siRNA H20 Plant diseases Grapevine red globe virus (GRGV) is a member of the genus Maculavirus, family Tymoviridae, found to infect grapevine for the first time in Italy (Sabanadzovic et al. 2000). GRGV has also been reported in Greece, the United States, France, China, and Spain (Cretazzo et al. 2017; Fan et al. 2016). No specific symptoms have been associated with GRGV infection (Martelli et al. 2002). In 2016, grapevine leaves from a Pinot Gris cultivar from the Rhineland-Palatinate area showing leaf mottling and flecking were collected and analyzed by high-throughput sequencing of siRNAs using TrueSeq Illumina technology on a HiSeq 1000 system. De novo assembly of the 5,658,588 reads was performed by Geneious software. The BlastN and BlastX analysis of the 316 contigs obtained revealed the presence of 65 contigs related to GRGV, with a percentage of nucleotide similarity ranging from 85.2 to 100%. Illumina reads were mapped to reference using the Graciano-T101 isolate (GenBank accession no. NC_030693.1), allowing the recovery of 90.1% of the viral sequence scattered along the genome. A partial sequence corresponding to a fragment of the GRGV replicase gene of one of the German isolates has been deposited in GenBank database (accession no. MG779496). Bioinformatic analysis of the remaining contigs showed the presence of Arabis mosaic virus, grapevine rupestris stem pitting associated virus, grapevine yellow speckle viroid-1, and hop stunt viroid. The presence of GRGV in the German vineyards was confirmed by reverse-transcription polymerase chain reaction (RT-PCR) using two different sets of primers, the generic primers for tymoviruses, 5′-CATGCANGTSAGRGGRCCRAA-3′ and 5′-CYCARCAYAARGTVAACGA-3′ (Kominek et al. 2009), and newly designed primers specific for GRGV, GRGV-CF-F1 5′-GAATTCGCTGTCGGCCACTC-3′ and GRGV-CF-R1 5′-AGTGAGAGGAGAGATTCCATC-3′. The expected RT-PCR products, of 453 and 386 bp respectively, were successfully amplified, cloned, and sequenced. BlastN analysis of all amplicons confirmed the presence of the virus. GRGV was also detected by RT-PCR in 14 out of 57 randomly collected samples from symptomatic and asymptomatic grapevines, in different grapevine growing areas from the Rhineland-Palatinate area. To our knowledge, this is the first report of GRGV in Germany. Further studies will be necessary to assess the relevance and potential threat of this virus to the grape production in Germany and to determine whether it should be regulated. 2019-01-14T13:58:13Z 2019-01-14T13:58:13Z 2018 article A. B. Ruiz-García, S. Nourinejhad Zarghani, A. Okic, A. Olmos, T. Wetzel (2018) First report of Grapevine Red Globe virus in Grapevine in Germany. Plant Disease 102:8, 1675 1943-7692 http://hdl.handle.net/20.500.11939/6154 10.1094/PDIS-01-18-0105-PDN https://apsjournals.apsnet.org/doi/10.1094/PDIS-01-18-0105-PDN en_US Info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/RTA2014-00061-C03-01 COST Action FA1407 (DIVAS), RTA2014-00061-C03-01 closedAccess The American Phytopathological Society electronico |
| spellingShingle | Grapevine red globe virus NGS Illumina siRNA H20 Plant diseases Ruiz-García, Ana B. Nourinejhad Zarghani, Shaheen Okic, Arnela Olmos, Antonio Wetzel, Thierry First Report of Grapevine Red Globe Virus in Grapevine in Germany |
| title | First Report of Grapevine Red Globe Virus in Grapevine in Germany |
| title_full | First Report of Grapevine Red Globe Virus in Grapevine in Germany |
| title_fullStr | First Report of Grapevine Red Globe Virus in Grapevine in Germany |
| title_full_unstemmed | First Report of Grapevine Red Globe Virus in Grapevine in Germany |
| title_short | First Report of Grapevine Red Globe Virus in Grapevine in Germany |
| title_sort | first report of grapevine red globe virus in grapevine in germany |
| topic | Grapevine red globe virus NGS Illumina siRNA H20 Plant diseases |
| url | http://hdl.handle.net/20.500.11939/6154 https://apsjournals.apsnet.org/doi/10.1094/PDIS-01-18-0105-PDN |
| work_keys_str_mv | AT ruizgarciaanab firstreportofgrapevineredglobevirusingrapevineingermany AT nourinejhadzarghanishaheen firstreportofgrapevineredglobevirusingrapevineingermany AT okicarnela firstreportofgrapevineredglobevirusingrapevineingermany AT olmosantonio firstreportofgrapevineredglobevirusingrapevineingermany AT wetzelthierry firstreportofgrapevineredglobevirusingrapevineingermany |