‘Candidatus Liberibacter solanacearum’ haplotypes D and E in carrot plants and seeds in Tunisia
Vegetative disorders similar to those associated with the presence of ‘Candidatus Liberibacter solanacearum’ (CaLsol) were observed in carrot plants in Kairouan, Tunisia from 2014 to 2016. Symptoms including leaf curling, yellowing, bronze and purplish discoloration, stunting of plants and roots, an...
| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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Springer
2018
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/6141 https://link.springer.com/article/10.1007/s42161-018-0045-7 |
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| author | Morán, Félix Ben Othmen, Soukaina Navarro-Herrero, Inmaculada Barbé, Silvia Martínez, M. Carmen Marco-Noales, Ester Chermiti, Brahim López, María M. |
| author_browse | Barbé, Silvia Ben Othmen, Soukaina Chermiti, Brahim López, María M. Marco-Noales, Ester Martínez, M. Carmen Morán, Félix Navarro-Herrero, Inmaculada |
| author_facet | Morán, Félix Ben Othmen, Soukaina Navarro-Herrero, Inmaculada Barbé, Silvia Martínez, M. Carmen Marco-Noales, Ester Chermiti, Brahim López, María M. |
| author_sort | Morán, Félix |
| collection | ReDivia |
| description | Vegetative disorders similar to those associated with the presence of ‘Candidatus Liberibacter solanacearum’ (CaLsol) were observed in carrot plants in Kairouan, Tunisia from 2014 to 2016. Symptoms including leaf curling, yellowing, bronze and purplish discoloration, stunting of plants and roots, and proliferation of secondary roots, affected 20 to 40% of the carrots in some plots. In order to determine if these symptoms were associated with the presence of CaLsol, and/or ‘Ca. Phytoplasma spp.’ and/or Spiroplasma citri, real-time PCR analyses were conducted using specific primers for these pathogens. CaLsol was detected for the first time in Tunisia and for the first time its haplotypes D and E were detected co-infecting a carrot plant. Furthermore, three samples of carrot seed produced in Kairouan tested positive for the haplotype D, showing a high percentage (35 to 63%) of viable bacterial cells after treatment with propidium monoazide. However, all the tests were negative for ‘Ca. Phytoplasma’ spp. as well as for S. citri. The results highlight that several CaLsol haplotypes are emerging carrot pathogens in new areas. |
| format | article |
| id | ReDivia6141 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | ReDivia61412025-04-25T14:46:18Z ‘Candidatus Liberibacter solanacearum’ haplotypes D and E in carrot plants and seeds in Tunisia Morán, Félix Ben Othmen, Soukaina Navarro-Herrero, Inmaculada Barbé, Silvia Martínez, M. Carmen Marco-Noales, Ester Chermiti, Brahim López, María M. ‘Ca. Phytoplasma’ spp. Spiroplasma citri Bactericera sp. real-time PCR H20 Plant diseases Vegetative disorders similar to those associated with the presence of ‘Candidatus Liberibacter solanacearum’ (CaLsol) were observed in carrot plants in Kairouan, Tunisia from 2014 to 2016. Symptoms including leaf curling, yellowing, bronze and purplish discoloration, stunting of plants and roots, and proliferation of secondary roots, affected 20 to 40% of the carrots in some plots. In order to determine if these symptoms were associated with the presence of CaLsol, and/or ‘Ca. Phytoplasma spp.’ and/or Spiroplasma citri, real-time PCR analyses were conducted using specific primers for these pathogens. CaLsol was detected for the first time in Tunisia and for the first time its haplotypes D and E were detected co-infecting a carrot plant. Furthermore, three samples of carrot seed produced in Kairouan tested positive for the haplotype D, showing a high percentage (35 to 63%) of viable bacterial cells after treatment with propidium monoazide. However, all the tests were negative for ‘Ca. Phytoplasma’ spp. as well as for S. citri. The results highlight that several CaLsol haplotypes are emerging carrot pathogens in new areas. 2018-12-03T14:55:58Z 2018-12-03T14:55:58Z 2018 article publishedVersion Ben Othmen, S., Morán, F., Navarro, I., Barbé, S., Martínez, C., Marco-Noales, E. et al. (2018). ‘Candidatus Liberibacter solanacearum’ haplotypes D and E in carrot plants and seeds in Tunisia. Journal of Plant Pathology, 100(2), 197-207. 1125-4653 http://hdl.handle.net/20.500.11939/6141 10.1007/s42161-018-0045-7 https://link.springer.com/article/10.1007/s42161-018-0045-7 en Springer electronico |
| spellingShingle | ‘Ca. Phytoplasma’ spp. Spiroplasma citri Bactericera sp. real-time PCR H20 Plant diseases Morán, Félix Ben Othmen, Soukaina Navarro-Herrero, Inmaculada Barbé, Silvia Martínez, M. Carmen Marco-Noales, Ester Chermiti, Brahim López, María M. ‘Candidatus Liberibacter solanacearum’ haplotypes D and E in carrot plants and seeds in Tunisia |
| title | ‘Candidatus Liberibacter solanacearum’ haplotypes D and E in carrot plants and seeds in Tunisia |
| title_full | ‘Candidatus Liberibacter solanacearum’ haplotypes D and E in carrot plants and seeds in Tunisia |
| title_fullStr | ‘Candidatus Liberibacter solanacearum’ haplotypes D and E in carrot plants and seeds in Tunisia |
| title_full_unstemmed | ‘Candidatus Liberibacter solanacearum’ haplotypes D and E in carrot plants and seeds in Tunisia |
| title_short | ‘Candidatus Liberibacter solanacearum’ haplotypes D and E in carrot plants and seeds in Tunisia |
| title_sort | candidatus liberibacter solanacearum haplotypes d and e in carrot plants and seeds in tunisia |
| topic | ‘Ca. Phytoplasma’ spp. Spiroplasma citri Bactericera sp. real-time PCR H20 Plant diseases |
| url | http://hdl.handle.net/20.500.11939/6141 https://link.springer.com/article/10.1007/s42161-018-0045-7 |
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