Identification of a new polymorphism in the MP/CP region of Spanish isolates of Grapevine Pinot gris virus
Grapevine Pinot gris virus (GPGV) genome consists of one molecule of positive single stranded RNA that contains three overlapping open reading frames (ORFs) and two untranslated regions (5’ and 3’ UTRs). ORF1 encodes a methyltransferase, a helicase and the RNA dependent RNA polymerase (RdRp),ORF2...
| Main Authors: | , , , , , , |
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| Format: | poster |
| Language: | Inglés |
| Published: |
2018
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11939/6138 http://www.congresosef2018.es/librodeabstractsdelxixcongresodelasociedadespaaoladefitopatologia/! |
| Summary: | Grapevine Pinot gris virus (GPGV) genome consists of one molecule of positive single stranded
RNA that contains three overlapping open reading frames (ORFs) and two untranslated
regions (5’ and 3’ UTRs). ORF1 encodes a methyltransferase, a helicase and the RNA
dependent RNA polymerase (RdRp),ORF2 encodes the movement protein (MP) and ORF3
encodes the coat protein (CP). Several studies have reported the existence of high
variability in GPGV genomes. Among this genomic diversity, a polymorphism involving a stop
codon that produces a six amino acids shorter MP has been previously described. This
polymorphism has been related to the symptoms observed in GPGV infected grapevines.
In this study the genomic variability of MP/CP region, symptomatology and phylogenetic
relationships of 12 Spanish isolates have been determined. A new polymorphism has been
identified in the MP/CP region of some Spanish isolates. It involves the presence of a stop
codon signal three nucleotides downstream from the polymorphism previously reported and
would determine the synthesis of a five amino acids shorter MP. However, the phylogenetic
study of these genome variants showed that the new polymorphism clusters in a different clade
than the previously reported one, suggesting that these two polymorphisms, that result in a
similar shortening of the GPGV MP, are independent evolutionary events. No association
between the presence of these genetic variants and the symptomatology of Spanish GPGV
infected grapevines has been found, suggesting that other factors would be involved in the
epidemiology of the GPGV associated disease. These results give new insights in the study
of GPGV genomic variability. |
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