Detection of Southern tomato virus by molecular hybridisation
Southern tomato virus (STV) is a double‐stranded RNA (dsRNA) virus belonging to the genus Amalgavirus from the family Amalgamaviridae. STV has been detected in tomato plants showing symptoms of stunting, fruit discoloration and size reduction, although its role on symptom development is unclear. Als...
| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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2018
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/6085 https://onlinelibrary.wiley.com/doi/full/10.1111/aab.12367 |
| _version_ | 1855032364045959168 |
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| author | Puchades, Andrés V. Carpino, Caterina Alfaro-Fernández, Ana Font-San-Ambrosio, Isabel Davino, Salvatore Guerri, José Rubio, Luis Galipienso, Luis |
| author_browse | Alfaro-Fernández, Ana Carpino, Caterina Davino, Salvatore Font-San-Ambrosio, Isabel Galipienso, Luis Guerri, José Puchades, Andrés V. Rubio, Luis |
| author_facet | Puchades, Andrés V. Carpino, Caterina Alfaro-Fernández, Ana Font-San-Ambrosio, Isabel Davino, Salvatore Guerri, José Rubio, Luis Galipienso, Luis |
| author_sort | Puchades, Andrés V. |
| collection | ReDivia |
| description | Southern tomato virus (STV) is a double‐stranded RNA (dsRNA) virus belonging to the genus Amalgavirus from the family Amalgamaviridae. STV has been detected in tomato plants showing symptoms of stunting, fruit discoloration and size reduction, although its role on symptom development is unclear. Also, little is known about the incidence and epidemiology of this virus and how it spreads in tomato crops. In this work, we developed a molecular hybridisation method by using a digoxigenin‐labelled RNA probe based on the nucleotide sequence of the STV putative coat protein which was tested with different procedures for preparation of plant material. This technique was sensitive enough to detect STV from sap extracts (obtained just by grinding in buffer) from different plant tissues such as leaves, fruits, roots and seeds. This procedure is suitable for field surveys since it allows a cheap and quick processing of a high number of samples. Surveys performed in three important tomato production areas (Peninsular Spain, the Canary Islands and Sicily) showed that STV is widely spread, with incidences ranging from 18% to 74% in different local and commercial tomato varieties. |
| format | article |
| id | ReDivia6085 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | ReDivia60852025-04-25T14:46:09Z Detection of Southern tomato virus by molecular hybridisation Puchades, Andrés V. Carpino, Caterina Alfaro-Fernández, Ana Font-San-Ambrosio, Isabel Davino, Salvatore Guerri, José Rubio, Luis Galipienso, Luis Southern tomato virus (STV) is a double‐stranded RNA (dsRNA) virus belonging to the genus Amalgavirus from the family Amalgamaviridae. STV has been detected in tomato plants showing symptoms of stunting, fruit discoloration and size reduction, although its role on symptom development is unclear. Also, little is known about the incidence and epidemiology of this virus and how it spreads in tomato crops. In this work, we developed a molecular hybridisation method by using a digoxigenin‐labelled RNA probe based on the nucleotide sequence of the STV putative coat protein which was tested with different procedures for preparation of plant material. This technique was sensitive enough to detect STV from sap extracts (obtained just by grinding in buffer) from different plant tissues such as leaves, fruits, roots and seeds. This procedure is suitable for field surveys since it allows a cheap and quick processing of a high number of samples. Surveys performed in three important tomato production areas (Peninsular Spain, the Canary Islands and Sicily) showed that STV is widely spread, with incidences ranging from 18% to 74% in different local and commercial tomato varieties. 2018-05-09T16:31:01Z 2018-05-09T16:31:01Z 2017 article Puchades, A.V., Carpino, C., Alfaro‐Fernandez, A., Font‐San‐Ambrosio, M. I., Davino, S., Guerri, J., Rubio, L., Galipienso, L. (2017). Detection of southern tomato virus by molecular hybridisation. Annals of Applied Biology, 171(2), 172-178. http://hdl.handle.net/20.500.11939/6085 10.1111/aab.12367 https://onlinelibrary.wiley.com/doi/full/10.1111/aab.12367 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ electronico |
| spellingShingle | Puchades, Andrés V. Carpino, Caterina Alfaro-Fernández, Ana Font-San-Ambrosio, Isabel Davino, Salvatore Guerri, José Rubio, Luis Galipienso, Luis Detection of Southern tomato virus by molecular hybridisation |
| title | Detection of Southern tomato virus by molecular hybridisation |
| title_full | Detection of Southern tomato virus by molecular hybridisation |
| title_fullStr | Detection of Southern tomato virus by molecular hybridisation |
| title_full_unstemmed | Detection of Southern tomato virus by molecular hybridisation |
| title_short | Detection of Southern tomato virus by molecular hybridisation |
| title_sort | detection of southern tomato virus by molecular hybridisation |
| url | http://hdl.handle.net/20.500.11939/6085 https://onlinelibrary.wiley.com/doi/full/10.1111/aab.12367 |
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