Cell Wall Remodeling in Abscission Zone Cells during Ethylene-Promoted Fruit Abscission in Citrus
Abscission is a cell separation process by which plants can shed organs such as fruits, leaves, or flowers. The process takes place in specific locations termed abscission zones. In fruit crops like citrus, fruit abscission represents a high percentage of annual yield losses. Thus, understanding...
| Main Authors: | , , , , , , , , , , , |
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| Format: | article |
| Language: | Inglés |
| Published: |
2018
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| Online Access: | http://hdl.handle.net/20.500.11939/6073 https://www.frontiersin.org/articles/10.3389/fpls.2017.00126/full |
| _version_ | 1855032362023256064 |
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| author | Merelo, Paz Agustí, Javier Arbona, Vicent Costa, Mario L. Estornell, Leandro H. Gómez-Cadenas, Aurelio Coimbra, Silvia Gómez, María D. Pérez-Amador, Miguel A. Domingo, Concha Talón, Manuel Tadeo, Francisco R. |
| author_browse | Agustí, Javier Arbona, Vicent Coimbra, Silvia Costa, Mario L. Domingo, Concha Estornell, Leandro H. Gómez, María D. Gómez-Cadenas, Aurelio Merelo, Paz Pérez-Amador, Miguel A. Tadeo, Francisco R. Talón, Manuel |
| author_facet | Merelo, Paz Agustí, Javier Arbona, Vicent Costa, Mario L. Estornell, Leandro H. Gómez-Cadenas, Aurelio Coimbra, Silvia Gómez, María D. Pérez-Amador, Miguel A. Domingo, Concha Talón, Manuel Tadeo, Francisco R. |
| author_sort | Merelo, Paz |
| collection | ReDivia |
| description | Abscission is a cell separation process by which plants can shed organs such as fruits,
leaves, or flowers. The process takes place in specific locations termed abscission zones.
In fruit crops like citrus, fruit abscission represents a high percentage of annual yield
losses. Thus, understanding the molecular regulation of abscission is of capital relevance
to control production. To identify genes preferentially expressed within the citrus fruit
abscission zone (AZ-C), we performed a comparative transcriptomics assay at the cell
type resolution level between the AZ-C and adjacent fruit rind cells (non-abscising tissue)
during ethylene-promoted abscission. Our strategy combined laser microdissection with
microarray analysis. Cell wall modification-related gene families displayed prominent
representation in the AZ-C. Phylogenetic analyses of such gene families revealed a link
between phylogenetic proximity and expression pattern during abscission suggesting
highly conserved roles for specific members of these families in abscission. Our
transcriptomic data was validated with (and strongly supported by) a parallel approach
consisting on anatomical, histochemical and biochemical analyses on the AZ-C during
fruit abscission. Our work identifies genes potentially involved in organ abscission and
provides relevant data for future biotechnology approaches aimed at controlling such
crucial process for citrus yield. |
| format | article |
| id | ReDivia6073 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | ReDivia60732025-04-25T14:46:07Z Cell Wall Remodeling in Abscission Zone Cells during Ethylene-Promoted Fruit Abscission in Citrus Merelo, Paz Agustí, Javier Arbona, Vicent Costa, Mario L. Estornell, Leandro H. Gómez-Cadenas, Aurelio Coimbra, Silvia Gómez, María D. Pérez-Amador, Miguel A. Domingo, Concha Talón, Manuel Tadeo, Francisco R. Abscission is a cell separation process by which plants can shed organs such as fruits, leaves, or flowers. The process takes place in specific locations termed abscission zones. In fruit crops like citrus, fruit abscission represents a high percentage of annual yield losses. Thus, understanding the molecular regulation of abscission is of capital relevance to control production. To identify genes preferentially expressed within the citrus fruit abscission zone (AZ-C), we performed a comparative transcriptomics assay at the cell type resolution level between the AZ-C and adjacent fruit rind cells (non-abscising tissue) during ethylene-promoted abscission. Our strategy combined laser microdissection with microarray analysis. Cell wall modification-related gene families displayed prominent representation in the AZ-C. Phylogenetic analyses of such gene families revealed a link between phylogenetic proximity and expression pattern during abscission suggesting highly conserved roles for specific members of these families in abscission. Our transcriptomic data was validated with (and strongly supported by) a parallel approach consisting on anatomical, histochemical and biochemical analyses on the AZ-C during fruit abscission. Our work identifies genes potentially involved in organ abscission and provides relevant data for future biotechnology approaches aimed at controlling such crucial process for citrus yield. 2018-05-09T16:31:00Z 2018-05-09T16:31:00Z 2017 article publishedVersion Merelo, P., Agusti, J., Arbona, V., Costa, M. L., Estornell, L. H., Gomez-Cadenas, A., Coimbra, S., Gomez, M. D., Perez-Amador, M. A., Domingo, C., Talon, M., Tadeo, F. R. (2017). Cell wall remodeling in abscission zone cells during ethylene-promoted fruit abscission in citrus. Frontiers in Plant Science, 8, 126. 1664-462X http://hdl.handle.net/20.500.11939/6073 10.3389/fpls.2017.00126 https://www.frontiersin.org/articles/10.3389/fpls.2017.00126/full en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ electronico |
| spellingShingle | Merelo, Paz Agustí, Javier Arbona, Vicent Costa, Mario L. Estornell, Leandro H. Gómez-Cadenas, Aurelio Coimbra, Silvia Gómez, María D. Pérez-Amador, Miguel A. Domingo, Concha Talón, Manuel Tadeo, Francisco R. Cell Wall Remodeling in Abscission Zone Cells during Ethylene-Promoted Fruit Abscission in Citrus |
| title | Cell Wall Remodeling in Abscission Zone Cells during Ethylene-Promoted Fruit Abscission in Citrus |
| title_full | Cell Wall Remodeling in Abscission Zone Cells during Ethylene-Promoted Fruit Abscission in Citrus |
| title_fullStr | Cell Wall Remodeling in Abscission Zone Cells during Ethylene-Promoted Fruit Abscission in Citrus |
| title_full_unstemmed | Cell Wall Remodeling in Abscission Zone Cells during Ethylene-Promoted Fruit Abscission in Citrus |
| title_short | Cell Wall Remodeling in Abscission Zone Cells during Ethylene-Promoted Fruit Abscission in Citrus |
| title_sort | cell wall remodeling in abscission zone cells during ethylene promoted fruit abscission in citrus |
| url | http://hdl.handle.net/20.500.11939/6073 https://www.frontiersin.org/articles/10.3389/fpls.2017.00126/full |
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