Rootstock influence on iron uptake responses in Citrus leaves and their regulation under the Fe paradox effect
Background and aims: This work evaluates the regulation of iron uptake responses in Citrus leaves and their involvement in the Fe paradox effect. Methods: Experiments were performed in field-grown 'Navelina' trees grafted onto two Cleopatra mandarin x Poncirus trifoliata (L.) Raf. hybrids with diffe...
| Autores principales: | , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
|
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6069 |
| _version_ | 1855492012256526336 |
|---|---|
| author | Martínez-Cuenca, Mary-Rus Primo-Capella, Amparo Quinones, Ana Bermejo, Almudena Forner-Giner, María A. |
| author_browse | Bermejo, Almudena Forner-Giner, María A. Martínez-Cuenca, Mary-Rus Primo-Capella, Amparo Quinones, Ana |
| author_facet | Martínez-Cuenca, Mary-Rus Primo-Capella, Amparo Quinones, Ana Bermejo, Almudena Forner-Giner, María A. |
| author_sort | Martínez-Cuenca, Mary-Rus |
| collection | ReDivia |
| description | Background and aims: This work evaluates the regulation of iron uptake responses in Citrus leaves and their involvement in the Fe paradox effect. Methods: Experiments were performed in field-grown 'Navelina' trees grafted onto two Cleopatra mandarin x Poncirus trifoliata (L.) Raf. hybrids with different Fe-chlorosis symptoms: 030146 (non-chlorotic) and 030122 (chlorotic). Results: Chlorotic leaves were smaller than non-chlorotic ones for both dry weight (DW) and area basis, and exhibited marked photosynthetic state affection, but reduced catalase and peroxidase enzymatic activities. Although both samples had a similar total Fe concentration on DW, it was lower in chlorotic leaves when expressed on an area basis. A similar pattern was observed for the total Fe concentration in the apoplast and cell sap and in active Fe (Fe2+) concentration. FRO2 gene expression and ferric chelate reductase (FC-R) activity were also lower in chlorotic samples, while HA1 and IRT1 were more induced. Despite similar apoplasmic pH, K+/Ca2+ was higher in chlorotic leaves, and both citrate and malate concentrations in total tissue and apoplast fluid were lower. Conclusion: (1) The rootstock influences Fe acquisition system in the leaf; (2) the increased sensitivity to Fe-deficiency as revealed by chlorosis and decreased biomass, was correlated with lower FC-R activity and lower organic acid level in leaf cells, which could cause a decreased Fe mobility and trigger other Fe-stress responses in this organ to enhance acidification and Fe uptake inside cells; and (3) the chlorosis paradox phenomenon in citrus likely occurs as a combination of a marked FC-R activity impairment in the leaf and the strong growth inhibition in this organ. |
| format | Artículo |
| id | ReDivia6069 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | ReDivia60692025-04-25T14:46:06Z Rootstock influence on iron uptake responses in Citrus leaves and their regulation under the Fe paradox effect Martínez-Cuenca, Mary-Rus Primo-Capella, Amparo Quinones, Ana Bermejo, Almudena Forner-Giner, María A. Background and aims: This work evaluates the regulation of iron uptake responses in Citrus leaves and their involvement in the Fe paradox effect. Methods: Experiments were performed in field-grown 'Navelina' trees grafted onto two Cleopatra mandarin x Poncirus trifoliata (L.) Raf. hybrids with different Fe-chlorosis symptoms: 030146 (non-chlorotic) and 030122 (chlorotic). Results: Chlorotic leaves were smaller than non-chlorotic ones for both dry weight (DW) and area basis, and exhibited marked photosynthetic state affection, but reduced catalase and peroxidase enzymatic activities. Although both samples had a similar total Fe concentration on DW, it was lower in chlorotic leaves when expressed on an area basis. A similar pattern was observed for the total Fe concentration in the apoplast and cell sap and in active Fe (Fe2+) concentration. FRO2 gene expression and ferric chelate reductase (FC-R) activity were also lower in chlorotic samples, while HA1 and IRT1 were more induced. Despite similar apoplasmic pH, K+/Ca2+ was higher in chlorotic leaves, and both citrate and malate concentrations in total tissue and apoplast fluid were lower. Conclusion: (1) The rootstock influences Fe acquisition system in the leaf; (2) the increased sensitivity to Fe-deficiency as revealed by chlorosis and decreased biomass, was correlated with lower FC-R activity and lower organic acid level in leaf cells, which could cause a decreased Fe mobility and trigger other Fe-stress responses in this organ to enhance acidification and Fe uptake inside cells; and (3) the chlorosis paradox phenomenon in citrus likely occurs as a combination of a marked FC-R activity impairment in the leaf and the strong growth inhibition in this organ. 2018-05-09T16:31:00Z 2018-05-09T16:31:00Z 2017 article publishedVersion Martinez-Cuenca, M., Primo-Capella, A., Quinones, A., Bermejo, A., Forner-Giner, M. A. (2017). Rootstock influence on iron uptake responses in citrus leaves and their regulation under the fe paradox effect. Peerj, 5, e3553. 2167-8359 http://hdl.handle.net/20.500.11939/6069 10.7717/peerj.3553 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ electronico |
| spellingShingle | Martínez-Cuenca, Mary-Rus Primo-Capella, Amparo Quinones, Ana Bermejo, Almudena Forner-Giner, María A. Rootstock influence on iron uptake responses in Citrus leaves and their regulation under the Fe paradox effect |
| title | Rootstock influence on iron uptake responses in Citrus leaves and their regulation under the Fe paradox effect |
| title_full | Rootstock influence on iron uptake responses in Citrus leaves and their regulation under the Fe paradox effect |
| title_fullStr | Rootstock influence on iron uptake responses in Citrus leaves and their regulation under the Fe paradox effect |
| title_full_unstemmed | Rootstock influence on iron uptake responses in Citrus leaves and their regulation under the Fe paradox effect |
| title_short | Rootstock influence on iron uptake responses in Citrus leaves and their regulation under the Fe paradox effect |
| title_sort | rootstock influence on iron uptake responses in citrus leaves and their regulation under the fe paradox effect |
| url | http://hdl.handle.net/20.500.11939/6069 |
| work_keys_str_mv | AT martinezcuencamaryrus rootstockinfluenceonironuptakeresponsesincitrusleavesandtheirregulationunderthefeparadoxeffect AT primocapellaamparo rootstockinfluenceonironuptakeresponsesincitrusleavesandtheirregulationunderthefeparadoxeffect AT quinonesana rootstockinfluenceonironuptakeresponsesincitrusleavesandtheirregulationunderthefeparadoxeffect AT bermejoalmudena rootstockinfluenceonironuptakeresponsesincitrusleavesandtheirregulationunderthefeparadoxeffect AT fornerginermariaa rootstockinfluenceonironuptakeresponsesincitrusleavesandtheirregulationunderthefeparadoxeffect |