Dual regulation of water retention and cell growth by a stressassociated protein (SAP) gene in Prunus
We have identified a gene (PpSAP1) of Prunus persica coding for a stress-associated protein (SAP) containing Zn-finger domains A20 and AN1. SAPs have been described as regulators of the abiotic stress response in plant species, emerging as potential candidates for improvement of stress tolerance in...
| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/6060 |
| _version_ | 1855032359699611648 |
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| author | Lloret, Alba Conejero, Ana Leida, Carmen Petri, Cesar Gil-Muñoz, Francisco Burgos, Lorenzo Badenes, María L. Ríos, Gabino |
| author_browse | Badenes, María L. Burgos, Lorenzo Conejero, Ana Gil-Muñoz, Francisco Leida, Carmen Lloret, Alba Petri, Cesar Ríos, Gabino |
| author_facet | Lloret, Alba Conejero, Ana Leida, Carmen Petri, Cesar Gil-Muñoz, Francisco Burgos, Lorenzo Badenes, María L. Ríos, Gabino |
| author_sort | Lloret, Alba |
| collection | ReDivia |
| description | We have identified a gene (PpSAP1) of Prunus persica coding for a stress-associated protein (SAP) containing Zn-finger domains A20 and AN1. SAPs have been described as regulators of the abiotic stress response in plant species, emerging as potential candidates for improvement of stress tolerance in plants. PpSAP1 was highly expressed in leaves and dormant buds, being down-regulated before bud dormancy release. PpSAP1 expression was moderately induced by water stresses and heat in buds. In addition, it was found that PpSAP1 strongly interacts with polyubiquitin proteins in the yeast two-hybrid system. The overexpression of PpSAP1 in transgenic plum plants led to alterations in leaf shape and an increase of water retention under drought stress. Moreover, we established that leaf morphological alterations were concomitant with a reduced cell size and down-regulation of genes involved in cell growth, such as GROWTH-REGULATING FACTOR (GRF) 1-like, TONOPLAST INTRINSIC PROTEIN (TIP)-like, and TARGET OF RAPAMYCIN (TOR)-like. Especially, the inverse expression pattern of PpSAP1 and TOR-like in transgenic plum and peach buds suggests a role of PpSAP1 in cell expansion through the regulation of TOR pathway. |
| format | article |
| id | ReDivia6060 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | ReDivia60602025-04-25T14:46:04Z Dual regulation of water retention and cell growth by a stressassociated protein (SAP) gene in Prunus Lloret, Alba Conejero, Ana Leida, Carmen Petri, Cesar Gil-Muñoz, Francisco Burgos, Lorenzo Badenes, María L. Ríos, Gabino We have identified a gene (PpSAP1) of Prunus persica coding for a stress-associated protein (SAP) containing Zn-finger domains A20 and AN1. SAPs have been described as regulators of the abiotic stress response in plant species, emerging as potential candidates for improvement of stress tolerance in plants. PpSAP1 was highly expressed in leaves and dormant buds, being down-regulated before bud dormancy release. PpSAP1 expression was moderately induced by water stresses and heat in buds. In addition, it was found that PpSAP1 strongly interacts with polyubiquitin proteins in the yeast two-hybrid system. The overexpression of PpSAP1 in transgenic plum plants led to alterations in leaf shape and an increase of water retention under drought stress. Moreover, we established that leaf morphological alterations were concomitant with a reduced cell size and down-regulation of genes involved in cell growth, such as GROWTH-REGULATING FACTOR (GRF) 1-like, TONOPLAST INTRINSIC PROTEIN (TIP)-like, and TARGET OF RAPAMYCIN (TOR)-like. Especially, the inverse expression pattern of PpSAP1 and TOR-like in transgenic plum and peach buds suggests a role of PpSAP1 in cell expansion through the regulation of TOR pathway. 2018-05-09T16:30:59Z 2018-05-09T16:30:59Z 2017 article publishedVersion Lloret, A., Conejero, A., Leida, C., Petri, C., Gil-Munoz, F., Burgos, L. et al. (2017). Dual regulation of water retention and cell growth by a stressassociated protein (SAP) gene in prunus. Scientific Reports, 7, 332. 2045-2322 http://hdl.handle.net/20.500.11939/6060 10.1038/s41598-017-00471-7 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ electronico |
| spellingShingle | Lloret, Alba Conejero, Ana Leida, Carmen Petri, Cesar Gil-Muñoz, Francisco Burgos, Lorenzo Badenes, María L. Ríos, Gabino Dual regulation of water retention and cell growth by a stressassociated protein (SAP) gene in Prunus |
| title | Dual regulation of water retention and cell growth by a stressassociated protein (SAP) gene in Prunus |
| title_full | Dual regulation of water retention and cell growth by a stressassociated protein (SAP) gene in Prunus |
| title_fullStr | Dual regulation of water retention and cell growth by a stressassociated protein (SAP) gene in Prunus |
| title_full_unstemmed | Dual regulation of water retention and cell growth by a stressassociated protein (SAP) gene in Prunus |
| title_short | Dual regulation of water retention and cell growth by a stressassociated protein (SAP) gene in Prunus |
| title_sort | dual regulation of water retention and cell growth by a stressassociated protein sap gene in prunus |
| url | http://hdl.handle.net/20.500.11939/6060 |
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