The sodium transporter encoded by the HKT1;2 gene modulates sodium/potassium homeostasis in tomato shoots under salinity
Excessive soil salinity diminishes crop yield and quality. In a previous study in tomato, we identified two closely linked genes encoding HKT1-like transporters, HKT1;1 and HKT1;2, as candidate genes for a major quantitative trait locus (kc7.1) related to shoot Na+/K+ homeostasis - a major salt tole...
| Autores principales: | , , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/6052 |
| _version_ | 1855032358478020608 |
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| author | Jaime-Perez, Noelia Pineda, Benito Garcia-Sogo, Begona Atares, Alejandro Athman, Asmini Byrt, Caitlin S. Olias, Raquel Asins, María J. Gilliham, Matthew Moreno, Vicente Belver, Andres |
| author_browse | Asins, María J. Atares, Alejandro Athman, Asmini Belver, Andres Byrt, Caitlin S. Garcia-Sogo, Begona Gilliham, Matthew Jaime-Perez, Noelia Moreno, Vicente Olias, Raquel Pineda, Benito |
| author_facet | Jaime-Perez, Noelia Pineda, Benito Garcia-Sogo, Begona Atares, Alejandro Athman, Asmini Byrt, Caitlin S. Olias, Raquel Asins, María J. Gilliham, Matthew Moreno, Vicente Belver, Andres |
| author_sort | Jaime-Perez, Noelia |
| collection | ReDivia |
| description | Excessive soil salinity diminishes crop yield and quality. In a previous study in tomato, we identified two closely linked genes encoding HKT1-like transporters, HKT1;1 and HKT1;2, as candidate genes for a major quantitative trait locus (kc7.1) related to shoot Na+/K+ homeostasis - a major salt tolerance trait - using two populations of recombinant inbred lines (RILs). Here, we determine the effectiveness of these genes in conferring improved salt tolerance by using two near-isogenic lines (NILs) that were homozygous for either the Solanum lycopersicum allele (NIL17) or for the Solanum cheesmaniae allele (NIL14) at both HKT1 loci; transgenic lines derived from these NILs in which each HKT1;1 and HKT1;2 had been silenced by stable transformation were also used. Silencing of ScHKT1;2 and SlHKT1;2 altered the leaf Na+/K+ ratio and caused hypersensitivity to salinity in plants cultivated under transpiring conditions, whereas silencing SlHKT1;1/ScHKT1;1 had a lesser effect. These results indicate that HKT1;2 has the more significant role in Na+ homeostasis and salinity tolerance in tomato. |
| format | article |
| id | ReDivia6052 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | ReDivia60522025-04-25T14:46:02Z The sodium transporter encoded by the HKT1;2 gene modulates sodium/potassium homeostasis in tomato shoots under salinity Jaime-Perez, Noelia Pineda, Benito Garcia-Sogo, Begona Atares, Alejandro Athman, Asmini Byrt, Caitlin S. Olias, Raquel Asins, María J. Gilliham, Matthew Moreno, Vicente Belver, Andres Excessive soil salinity diminishes crop yield and quality. In a previous study in tomato, we identified two closely linked genes encoding HKT1-like transporters, HKT1;1 and HKT1;2, as candidate genes for a major quantitative trait locus (kc7.1) related to shoot Na+/K+ homeostasis - a major salt tolerance trait - using two populations of recombinant inbred lines (RILs). Here, we determine the effectiveness of these genes in conferring improved salt tolerance by using two near-isogenic lines (NILs) that were homozygous for either the Solanum lycopersicum allele (NIL17) or for the Solanum cheesmaniae allele (NIL14) at both HKT1 loci; transgenic lines derived from these NILs in which each HKT1;1 and HKT1;2 had been silenced by stable transformation were also used. Silencing of ScHKT1;2 and SlHKT1;2 altered the leaf Na+/K+ ratio and caused hypersensitivity to salinity in plants cultivated under transpiring conditions, whereas silencing SlHKT1;1/ScHKT1;1 had a lesser effect. These results indicate that HKT1;2 has the more significant role in Na+ homeostasis and salinity tolerance in tomato. 2018-05-09T16:30:58Z 2018-05-09T16:30:58Z 2017 article acceptedVersion Jaime-Perez, N., Pineda, B., Garcia-Sogo, B., Atares, A., Athman, A., Byrt, C. S. et al. (2017). The sodium transporter encoded by the HKT1,2 gene modulates sodium/potassium homeostasis in tomato shoots under salinity. Plant Cell and Environment, 40(5), 658-671. 0140-7791 http://hdl.handle.net/20.500.11939/6052 10.1111/pce.12883 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ electronico |
| spellingShingle | Jaime-Perez, Noelia Pineda, Benito Garcia-Sogo, Begona Atares, Alejandro Athman, Asmini Byrt, Caitlin S. Olias, Raquel Asins, María J. Gilliham, Matthew Moreno, Vicente Belver, Andres The sodium transporter encoded by the HKT1;2 gene modulates sodium/potassium homeostasis in tomato shoots under salinity |
| title | The sodium transporter encoded by the HKT1;2 gene modulates sodium/potassium homeostasis in tomato shoots under salinity |
| title_full | The sodium transporter encoded by the HKT1;2 gene modulates sodium/potassium homeostasis in tomato shoots under salinity |
| title_fullStr | The sodium transporter encoded by the HKT1;2 gene modulates sodium/potassium homeostasis in tomato shoots under salinity |
| title_full_unstemmed | The sodium transporter encoded by the HKT1;2 gene modulates sodium/potassium homeostasis in tomato shoots under salinity |
| title_short | The sodium transporter encoded by the HKT1;2 gene modulates sodium/potassium homeostasis in tomato shoots under salinity |
| title_sort | sodium transporter encoded by the hkt1 2 gene modulates sodium potassium homeostasis in tomato shoots under salinity |
| url | http://hdl.handle.net/20.500.11939/6052 |
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