The resistance of sour orange to Citrus tristeza virus is mediated by both the salicylic acid and RNA silencing defence pathways
Citrus tristeza virus (CTV) induces in the field the decline and death of citrus varieties grafted on sour orange (SO) rootstock, which has forced the use of alternative decline-tolerant rootstocks in affected countries, despite the highly desirable agronomic features of the SO rootstock. Declining...
| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/6047 |
| _version_ | 1855032357597216768 |
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| author | Gómez-Munoz, Neus Velázquez, Karelia Vives, María C. Ruiz-Ruiz, Susana Pina, José A. Flores, Ricardo Moreno, Pedro Guerri, José |
| author_browse | Flores, Ricardo Guerri, José Gómez-Munoz, Neus Moreno, Pedro Pina, José A. Ruiz-Ruiz, Susana Velázquez, Karelia Vives, María C. |
| author_facet | Gómez-Munoz, Neus Velázquez, Karelia Vives, María C. Ruiz-Ruiz, Susana Pina, José A. Flores, Ricardo Moreno, Pedro Guerri, José |
| author_sort | Gómez-Munoz, Neus |
| collection | ReDivia |
| description | Citrus tristeza virus (CTV) induces in the field the decline and death of citrus varieties grafted on sour orange (SO) rootstock, which has forced the use of alternative decline-tolerant rootstocks in affected countries, despite the highly desirable agronomic features of the SO rootstock. Declining citrus plants display phloem necrosis below the bud union. In addition, SO is minimally susceptible to CTV compared with other citrus varieties, suggesting partial resistance of SO to CTV. Here, by silencing different citrus genes with a Citrus leaf blotch virus-based vector, we have examined the implication of the RNA silencing and salicylic acid (SA) defence pathways in the resistance of SO to CTV. Silencing of the genes RDR1, NPR1 and DCL2/DCL4, associated with these defence pathways, enhanced virus spread and accumulation in SO plants in comparison with non-silenced controls, whereas silencing of the genes NPR3/NPR4, associated with the hypersensitive response, produced a slight decrease in CTV accumulation and reduced stunting of SO grafted on CTV-infected rough lemon plants. We also found that the CTV RNA silencing suppressors p20 and p23 also suppress the SA signalling defence, with the suppressor activity being higher in the most virulent isolates. |
| format | article |
| id | ReDivia6047 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | ReDivia60472025-04-25T14:46:01Z The resistance of sour orange to Citrus tristeza virus is mediated by both the salicylic acid and RNA silencing defence pathways Gómez-Munoz, Neus Velázquez, Karelia Vives, María C. Ruiz-Ruiz, Susana Pina, José A. Flores, Ricardo Moreno, Pedro Guerri, José Citrus tristeza virus (CTV) induces in the field the decline and death of citrus varieties grafted on sour orange (SO) rootstock, which has forced the use of alternative decline-tolerant rootstocks in affected countries, despite the highly desirable agronomic features of the SO rootstock. Declining citrus plants display phloem necrosis below the bud union. In addition, SO is minimally susceptible to CTV compared with other citrus varieties, suggesting partial resistance of SO to CTV. Here, by silencing different citrus genes with a Citrus leaf blotch virus-based vector, we have examined the implication of the RNA silencing and salicylic acid (SA) defence pathways in the resistance of SO to CTV. Silencing of the genes RDR1, NPR1 and DCL2/DCL4, associated with these defence pathways, enhanced virus spread and accumulation in SO plants in comparison with non-silenced controls, whereas silencing of the genes NPR3/NPR4, associated with the hypersensitive response, produced a slight decrease in CTV accumulation and reduced stunting of SO grafted on CTV-infected rough lemon plants. We also found that the CTV RNA silencing suppressors p20 and p23 also suppress the SA signalling defence, with the suppressor activity being higher in the most virulent isolates. 2018-05-09T16:30:58Z 2018-05-09T16:30:58Z 2017 article publishedVersion Gomez-Muñoz, N., Velazquez, K., Vives, M. C., Ruiz-Ruiz, S., Antonio Pina, J., Flores, R. et al. (2017). The resistance of sour orange to citrus tristeza virus is mediated by both the salicylic acid and RNA silencing defence pathways. Molecular Plant Pathology, 18(9), 1253-1266. 1464-6722 http://hdl.handle.net/20.500.11939/6047 10.1111/mpp.12488 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ electronico |
| spellingShingle | Gómez-Munoz, Neus Velázquez, Karelia Vives, María C. Ruiz-Ruiz, Susana Pina, José A. Flores, Ricardo Moreno, Pedro Guerri, José The resistance of sour orange to Citrus tristeza virus is mediated by both the salicylic acid and RNA silencing defence pathways |
| title | The resistance of sour orange to Citrus tristeza virus is mediated by both the salicylic acid and RNA silencing defence pathways |
| title_full | The resistance of sour orange to Citrus tristeza virus is mediated by both the salicylic acid and RNA silencing defence pathways |
| title_fullStr | The resistance of sour orange to Citrus tristeza virus is mediated by both the salicylic acid and RNA silencing defence pathways |
| title_full_unstemmed | The resistance of sour orange to Citrus tristeza virus is mediated by both the salicylic acid and RNA silencing defence pathways |
| title_short | The resistance of sour orange to Citrus tristeza virus is mediated by both the salicylic acid and RNA silencing defence pathways |
| title_sort | resistance of sour orange to citrus tristeza virus is mediated by both the salicylic acid and rna silencing defence pathways |
| url | http://hdl.handle.net/20.500.11939/6047 |
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