Search for reservoirs of 'Candidatus Liberibacter solanacearum' and mollicutes in weeds associated with carrot and celery crops
Currently, the main arthropod vectored pathogens associated with carrot and celery crop diseases are ˋCandidatus Liberibacter solanacearum´, Spiroplasma citri and different phytoplasma species. Mitigation strategies require elucidating whether these pathogens survive in the weeds of these Apiaceae c...
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2018
|
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6017 https://link.springer.com/article/10.1007%2Fs10658-016-0984-9 |
| _version_ | 1855032352358531072 |
|---|---|
| author | Alfaro-Fernández, Ana Verdeguer, Mercedes Rodríguez-León, Francisco Ibanez, Isabel Hernández, Desamparados Teresani, Gabriela Bertolini, Edson Cambra, Mariano Font-San-Ambrosio, Isabel |
| author_browse | Alfaro-Fernández, Ana Bertolini, Edson Cambra, Mariano Font-San-Ambrosio, Isabel Hernández, Desamparados Ibanez, Isabel Rodríguez-León, Francisco Teresani, Gabriela Verdeguer, Mercedes |
| author_facet | Alfaro-Fernández, Ana Verdeguer, Mercedes Rodríguez-León, Francisco Ibanez, Isabel Hernández, Desamparados Teresani, Gabriela Bertolini, Edson Cambra, Mariano Font-San-Ambrosio, Isabel |
| author_sort | Alfaro-Fernández, Ana |
| collection | ReDivia |
| description | Currently, the main arthropod vectored pathogens associated with carrot and celery crop diseases are ˋCandidatus Liberibacter solanacearum´, Spiroplasma citri and different phytoplasma species. Mitigation strategies require elucidating whether these pathogens survive in the weeds of these Apiaceae crops, which can act as reservoirs. Weed surveys were conducted in a vegetative cycle (April to October 2012) in the spontaneous vegetation that surrounded crops affected by ˋCa. L. solanacearum´, S. citri and/or phytoplasmas. Sixty-three species of 53 genera that belong to 23 botanical families were collected in the main carrot and celery Spanish production area. Species were identified, estimating coverage and abundance, and conserved in herbarium. Samples were analysed by nested-PCR with universal primers for phytoplasmas detection, and were sequenced for identification purposes; by conventional PCR for S. citri and real-time PCR for ˋCa. L. solanacearum´. The only detected pathogens were ˋCa. Phytoplasma trifolii´ (clover proliferation group 16Sr VI-A) in Amaranthus blitoides and Setaria adhaerens and ˋCa. P. solani´ (stolbur group 16Sr XII-A) in Convolvulus arvensis. These pathogens were also sporadically detected in celery or carrot crops. Unexpectedly, neither ˋCa. L. solanacearum´ nor S. citri was detected in the weed samples, despite the relatively high prevalence of these pathogens (less than 66 % and 25 %, respectively) in the surveyed plots. This suggests that weeds do not play an epidemiological role as reservoirs in the spread of such organisms in the studied region. The use of pathogen-free seed lots and the control of vectors are crucial for preventing the introduction and spread of these economical important pathogens to new areas. |
| format | article |
| id | ReDivia6017 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | ReDivia60172025-04-25T14:45:55Z Search for reservoirs of 'Candidatus Liberibacter solanacearum' and mollicutes in weeds associated with carrot and celery crops Alfaro-Fernández, Ana Verdeguer, Mercedes Rodríguez-León, Francisco Ibanez, Isabel Hernández, Desamparados Teresani, Gabriela Bertolini, Edson Cambra, Mariano Font-San-Ambrosio, Isabel Currently, the main arthropod vectored pathogens associated with carrot and celery crop diseases are ˋCandidatus Liberibacter solanacearum´, Spiroplasma citri and different phytoplasma species. Mitigation strategies require elucidating whether these pathogens survive in the weeds of these Apiaceae crops, which can act as reservoirs. Weed surveys were conducted in a vegetative cycle (April to October 2012) in the spontaneous vegetation that surrounded crops affected by ˋCa. L. solanacearum´, S. citri and/or phytoplasmas. Sixty-three species of 53 genera that belong to 23 botanical families were collected in the main carrot and celery Spanish production area. Species were identified, estimating coverage and abundance, and conserved in herbarium. Samples were analysed by nested-PCR with universal primers for phytoplasmas detection, and were sequenced for identification purposes; by conventional PCR for S. citri and real-time PCR for ˋCa. L. solanacearum´. The only detected pathogens were ˋCa. Phytoplasma trifolii´ (clover proliferation group 16Sr VI-A) in Amaranthus blitoides and Setaria adhaerens and ˋCa. P. solani´ (stolbur group 16Sr XII-A) in Convolvulus arvensis. These pathogens were also sporadically detected in celery or carrot crops. Unexpectedly, neither ˋCa. L. solanacearum´ nor S. citri was detected in the weed samples, despite the relatively high prevalence of these pathogens (less than 66 % and 25 %, respectively) in the surveyed plots. This suggests that weeds do not play an epidemiological role as reservoirs in the spread of such organisms in the studied region. The use of pathogen-free seed lots and the control of vectors are crucial for preventing the introduction and spread of these economical important pathogens to new areas. 2018-05-09T16:30:55Z 2018-05-09T16:30:55Z 2017 article Alfaro-Fernandez, A., Verdeguer, M., Rodríguez-León, F., Ibanez, I., Hernandez, D., Teresani, G. R., et al. (2017). Search for reservoirs of 'candidatus liberibacter solanacearum' and mollicutes in weeds associated with carrot and celery crops. European Journal of Plant Pathology, 147(1), 15-20. 0929-1873 http://hdl.handle.net/20.500.11939/6017 10.1007/s10658-016-0984-9 https://link.springer.com/article/10.1007%2Fs10658-016-0984-9 en closedAccess electronico |
| spellingShingle | Alfaro-Fernández, Ana Verdeguer, Mercedes Rodríguez-León, Francisco Ibanez, Isabel Hernández, Desamparados Teresani, Gabriela Bertolini, Edson Cambra, Mariano Font-San-Ambrosio, Isabel Search for reservoirs of 'Candidatus Liberibacter solanacearum' and mollicutes in weeds associated with carrot and celery crops |
| title | Search for reservoirs of 'Candidatus Liberibacter solanacearum' and mollicutes in weeds associated with carrot and celery crops |
| title_full | Search for reservoirs of 'Candidatus Liberibacter solanacearum' and mollicutes in weeds associated with carrot and celery crops |
| title_fullStr | Search for reservoirs of 'Candidatus Liberibacter solanacearum' and mollicutes in weeds associated with carrot and celery crops |
| title_full_unstemmed | Search for reservoirs of 'Candidatus Liberibacter solanacearum' and mollicutes in weeds associated with carrot and celery crops |
| title_short | Search for reservoirs of 'Candidatus Liberibacter solanacearum' and mollicutes in weeds associated with carrot and celery crops |
| title_sort | search for reservoirs of candidatus liberibacter solanacearum and mollicutes in weeds associated with carrot and celery crops |
| url | http://hdl.handle.net/20.500.11939/6017 https://link.springer.com/article/10.1007%2Fs10658-016-0984-9 |
| work_keys_str_mv | AT alfarofernandezana searchforreservoirsofcandidatusliberibactersolanacearumandmollicutesinweedsassociatedwithcarrotandcelerycrops AT verdeguermercedes searchforreservoirsofcandidatusliberibactersolanacearumandmollicutesinweedsassociatedwithcarrotandcelerycrops AT rodriguezleonfrancisco searchforreservoirsofcandidatusliberibactersolanacearumandmollicutesinweedsassociatedwithcarrotandcelerycrops AT ibanezisabel searchforreservoirsofcandidatusliberibactersolanacearumandmollicutesinweedsassociatedwithcarrotandcelerycrops AT hernandezdesamparados searchforreservoirsofcandidatusliberibactersolanacearumandmollicutesinweedsassociatedwithcarrotandcelerycrops AT teresanigabriela searchforreservoirsofcandidatusliberibactersolanacearumandmollicutesinweedsassociatedwithcarrotandcelerycrops AT bertoliniedson searchforreservoirsofcandidatusliberibactersolanacearumandmollicutesinweedsassociatedwithcarrotandcelerycrops AT cambramariano searchforreservoirsofcandidatusliberibactersolanacearumandmollicutesinweedsassociatedwithcarrotandcelerycrops AT fontsanambrosioisabel searchforreservoirsofcandidatusliberibactersolanacearumandmollicutesinweedsassociatedwithcarrotandcelerycrops |