Molecular characterization of new viral agents causing old citrus diseases by deep sequencing of small RNAs
There are some graft-transmissible citrus diseases such as incompatibility of varieties grafted on trifoliate rootstocks, impietratura and cristacortis that, although they have been described for many years in many citrus growing areas, their causal agent is still unknown and rapid detection metho...
| Autores principales: | , , , |
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| Formato: | conferenceObject |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5825 |
| _version_ | 1855032341142962176 |
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| author | Velázquez, Karelia Hervàs, J. Guerri, José Vives, María C. |
| author_browse | Guerri, José Hervàs, J. Velázquez, Karelia Vives, María C. |
| author_facet | Velázquez, Karelia Hervàs, J. Guerri, José Vives, María C. |
| author_sort | Velázquez, Karelia |
| collection | ReDivia |
| description | There are some graft-transmissible citrus diseases such as incompatibility of varieties grafted on trifoliate rootstocks, impietratura and
cristacortis that, although they have been described for many years in many citrus growing areas, their causal agent is still unknown
and rapid detection methods could not be developed. Diagnosis can only be made by means of infectivity assays on indicator pl ants,
resulting slow, expensive and non-specific and pose a limitation for the analysis of a large number of samples. These diseases are
present in Spain and their diagnosis is mandatory in Sanitation and Certification Programs. To identify the causal agent asso ciated to
such diseases and develop specific molecular detection methods, the small RNAs (sRNAs) fraction, extracted from Etrog citron plants
infected with each of the above diseases, was analyzed by deep sequencing. The assembly of the sRNAs derived from each sample
allowed the obtaining of several sequence fragments that were present in infected plants and not in healthy ones. In the case of
incompatibility on trifoliate rootstocks disease, we determined the complete genome sequence of a new virus provisionally nam ed
Trifoliate citrus bud union associated virus (TCBUaV), that showed homology with species of th e genus Cytorhabdovirus, whereas for
impietratura and cristacortis diseases we obtained partial sequences of individual viral genomes that had no homology with an y virus
available in the databases. Obtaining specific sequences of each pathogen has allowed us to develop specific and rapid molecular
detection methods thereof by RT-PCR |
| format | conferenceObject |
| id | ReDivia5825 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | ReDivia58252025-04-25T14:52:07Z Molecular characterization of new viral agents causing old citrus diseases by deep sequencing of small RNAs Velázquez, Karelia Hervàs, J. Guerri, José Vives, María C. There are some graft-transmissible citrus diseases such as incompatibility of varieties grafted on trifoliate rootstocks, impietratura and cristacortis that, although they have been described for many years in many citrus growing areas, their causal agent is still unknown and rapid detection methods could not be developed. Diagnosis can only be made by means of infectivity assays on indicator pl ants, resulting slow, expensive and non-specific and pose a limitation for the analysis of a large number of samples. These diseases are present in Spain and their diagnosis is mandatory in Sanitation and Certification Programs. To identify the causal agent asso ciated to such diseases and develop specific molecular detection methods, the small RNAs (sRNAs) fraction, extracted from Etrog citron plants infected with each of the above diseases, was analyzed by deep sequencing. The assembly of the sRNAs derived from each sample allowed the obtaining of several sequence fragments that were present in infected plants and not in healthy ones. In the case of incompatibility on trifoliate rootstocks disease, we determined the complete genome sequence of a new virus provisionally nam ed Trifoliate citrus bud union associated virus (TCBUaV), that showed homology with species of th e genus Cytorhabdovirus, whereas for impietratura and cristacortis diseases we obtained partial sequences of individual viral genomes that had no homology with an y virus available in the databases. Obtaining specific sequences of each pathogen has allowed us to develop specific and rapid molecular detection methods thereof by RT-PCR 2018-05-05T17:21:24Z 2018-05-05T17:21:24Z 2017 conferenceObject Velázquez, K., Hervàs, J., Guerri, J., Vives, M.C. (2017). Molecular characterization of new viral agents causing old citrus diseases by deep sequencing of small RNAs. In V Simposio Internacional de Fruticultura Tropical y Subtropical. http://hdl.handle.net/20.500.11939/5825 en 2017 V Simposio Internacional de Fruticultura Tropical y Subtropical Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ electronico |
| spellingShingle | Velázquez, Karelia Hervàs, J. Guerri, José Vives, María C. Molecular characterization of new viral agents causing old citrus diseases by deep sequencing of small RNAs |
| title | Molecular characterization of new viral agents causing old citrus diseases by deep sequencing of small RNAs |
| title_full | Molecular characterization of new viral agents causing old citrus diseases by deep sequencing of small RNAs |
| title_fullStr | Molecular characterization of new viral agents causing old citrus diseases by deep sequencing of small RNAs |
| title_full_unstemmed | Molecular characterization of new viral agents causing old citrus diseases by deep sequencing of small RNAs |
| title_short | Molecular characterization of new viral agents causing old citrus diseases by deep sequencing of small RNAs |
| title_sort | molecular characterization of new viral agents causing old citrus diseases by deep sequencing of small rnas |
| url | http://hdl.handle.net/20.500.11939/5825 |
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