Chitosan-dextran sulphate nanoparticles for GnRH release in rabbit insemination extenders
This study was designed to develop chitosan (CS)–dextran sulphate (DS) nanoparticles containing a GnRH analogue and to study their effect on rabbit (Oryctolagus cuniculus) semen quality. Six experimental extenders were tested as follows: (control) Tris‐citric acid‐glucose (TCG), (1) 0.05% CS‐0.05% D...
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| Formato: | conferenceObject |
| Lenguaje: | Inglés |
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2018
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5768 https://onlinelibrary.wiley.com/doi/abs/10.1111/rda.13062 |
| _version_ | 1855032331642863616 |
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| author | Fernández-Serrano, Paula Casares-Crespo, Lucía Viudes-De-Castro, María P. |
| author_browse | Casares-Crespo, Lucía Fernández-Serrano, Paula Viudes-De-Castro, María P. |
| author_facet | Fernández-Serrano, Paula Casares-Crespo, Lucía Viudes-De-Castro, María P. |
| author_sort | Fernández-Serrano, Paula |
| collection | ReDivia |
| description | This study was designed to develop chitosan (CS)–dextran sulphate (DS) nanoparticles containing a GnRH analogue and to study their effect on rabbit (Oryctolagus cuniculus) semen quality. Six experimental extenders were tested as follows: (control) Tris‐citric acid‐glucose (TCG), (1) 0.05% CS‐0.05% DS (4:1), (2) 0.1% CS‐0.05% DS (4:1), (3) 0.05% CS‐0.05% DS (3:1), (4) 0.1% CS‐0.05% DS (3:1), (5) 0.1% CS‐0.05% DS (2:1). CS and DS were dissolved in TCG medium, and nanoparticles were obtained through magnetic stirring. Rabbit seminal samples were incubated up to 5 hr at 37°C in the extenders, and seminal quality was evaluated. The entrapment efficiency was 40%–50%. After 5 hr at 37°C, a 20% of the hormone was released. Results showed that the presence of CS‐DS nanoparticles did not affect rabbit semen motility, viability and membrane functionality; however, acrosome integrity was significantly higher versus control (p < .001). |
| format | conferenceObject |
| id | ReDivia5768 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | ReDivia57682025-04-25T14:51:30Z Chitosan-dextran sulphate nanoparticles for GnRH release in rabbit insemination extenders Fernández-Serrano, Paula Casares-Crespo, Lucía Viudes-De-Castro, María P. L53 Animal physiology - Reproduction Chitosan Rabbits Semen This study was designed to develop chitosan (CS)–dextran sulphate (DS) nanoparticles containing a GnRH analogue and to study their effect on rabbit (Oryctolagus cuniculus) semen quality. Six experimental extenders were tested as follows: (control) Tris‐citric acid‐glucose (TCG), (1) 0.05% CS‐0.05% DS (4:1), (2) 0.1% CS‐0.05% DS (4:1), (3) 0.05% CS‐0.05% DS (3:1), (4) 0.1% CS‐0.05% DS (3:1), (5) 0.1% CS‐0.05% DS (2:1). CS and DS were dissolved in TCG medium, and nanoparticles were obtained through magnetic stirring. Rabbit seminal samples were incubated up to 5 hr at 37°C in the extenders, and seminal quality was evaluated. The entrapment efficiency was 40%–50%. After 5 hr at 37°C, a 20% of the hormone was released. Results showed that the presence of CS‐DS nanoparticles did not affect rabbit semen motility, viability and membrane functionality; however, acrosome integrity was significantly higher versus control (p < .001). 2018-04-26T14:12:48Z 2018-04-26T14:12:48Z 2017 conferenceObject Fernandez-Serrano, P., Casares-Crespo, L., Viudes-de-Castro, M.P. (2017). Chitosan-dextran sulphate nanoparticles for GnRH release in rabbit insemination extenders. In 14th International Congress of the Spanish Association for Animal Reproduction (AERA), Barcelona, Spain. http://hdl.handle.net/20.500.11939/5768 10.1111/rda.13062 https://onlinelibrary.wiley.com/doi/abs/10.1111/rda.13062 en 14th International Congress of the Spanish Association for Animal Reproduction (AERA) Barcelona, Spain Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ electronico |
| spellingShingle | L53 Animal physiology - Reproduction Chitosan Rabbits Semen Fernández-Serrano, Paula Casares-Crespo, Lucía Viudes-De-Castro, María P. Chitosan-dextran sulphate nanoparticles for GnRH release in rabbit insemination extenders |
| title | Chitosan-dextran sulphate nanoparticles for GnRH release in rabbit insemination extenders |
| title_full | Chitosan-dextran sulphate nanoparticles for GnRH release in rabbit insemination extenders |
| title_fullStr | Chitosan-dextran sulphate nanoparticles for GnRH release in rabbit insemination extenders |
| title_full_unstemmed | Chitosan-dextran sulphate nanoparticles for GnRH release in rabbit insemination extenders |
| title_short | Chitosan-dextran sulphate nanoparticles for GnRH release in rabbit insemination extenders |
| title_sort | chitosan dextran sulphate nanoparticles for gnrh release in rabbit insemination extenders |
| topic | L53 Animal physiology - Reproduction Chitosan Rabbits Semen |
| url | http://hdl.handle.net/20.500.11939/5768 https://onlinelibrary.wiley.com/doi/abs/10.1111/rda.13062 |
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