Chromatin-associated regulation of sorbitol synthesis in flower buds of peach
Key message PpeS6PDH gene is postulated to mediate sorbitol synthesis in flower buds of peach concomitantly with specific chromatin modifications. Abstract Perennial plants have evolved an adaptive mechanism involving protection of meristems within specialized structures named buds in order to surv...
| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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Springer
2017
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/5736 https://link.springer.com/article/10.1007/s11103-017-0669-6 |
| _version_ | 1855032326116868096 |
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| author | Lloret, Alba Martínez-Fuentes, Amparo Agustí, Manuel Badenes, María L. Ríos, Gabino |
| author_browse | Agustí, Manuel Badenes, María L. Lloret, Alba Martínez-Fuentes, Amparo Ríos, Gabino |
| author_facet | Lloret, Alba Martínez-Fuentes, Amparo Agustí, Manuel Badenes, María L. Ríos, Gabino |
| author_sort | Lloret, Alba |
| collection | ReDivia |
| description | Key message PpeS6PDH gene is postulated to mediate sorbitol synthesis in flower buds of peach concomitantly with specific chromatin modifications.
Abstract Perennial plants have evolved an adaptive mechanism involving protection of meristems within specialized structures named buds in order to survive low temperatures and water deprivation during winter. A seasonal period of dormancy further improves tolerance of buds to environmental stresses through specific mechanisms poorly known at the molecular level. We have shown that peach PpeS6PDH gene is down-regulated in flower buds after dormancy release, concomitantly with changes in the methylation level at specific lysine residues of histone H3 (H3K27 and H3K4) in the chromatin around the translation start site of the gene. PpeS6PDH encodes a NADPH-dependent sorbitol-6-phosphate dehydrogenase, the key enzyme for biosynthesis of sorbitol. Consistently, sorbitol accumulates in dormant buds showing higher PpeS6PDH expression. Moreover, PpeS6PDH gene expression is affected by cold and water deficit stress. Particularly, its expression is up-regulated by
low temperature in buds and leaves, whereas desiccation treatment induces PpeS6PDH in buds and represses the gene in leaves. These data reveal the concurrent participation of chromatin modification mechanisms, transcriptional regulation of PpeS6PDH and sorbitol accumulation in flower buds of peach. In addition to its role as a major translocatable
photosynthate in Rosaceae species, sorbitol is a widespread compatible solute and cryoprotectant, which suggests its participation in tolerance to environmental stresses in flower buds of peach. |
| format | article |
| id | ReDivia5736 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | ReDivia57362025-04-25T14:44:41Z Chromatin-associated regulation of sorbitol synthesis in flower buds of peach Lloret, Alba Martínez-Fuentes, Amparo Agustí, Manuel Badenes, María L. Ríos, Gabino Abiotic stress Bud dormancy Chromatin Prunus persica Sorbitol-6-phosphate dehydrogenase (S6PDH) Aldose-6-phosphate reductase (Ald6PRase) F30 Plant genetics and breeding Prunus persica Bud dormancy Abiotic stress Chromatin Key message PpeS6PDH gene is postulated to mediate sorbitol synthesis in flower buds of peach concomitantly with specific chromatin modifications. Abstract Perennial plants have evolved an adaptive mechanism involving protection of meristems within specialized structures named buds in order to survive low temperatures and water deprivation during winter. A seasonal period of dormancy further improves tolerance of buds to environmental stresses through specific mechanisms poorly known at the molecular level. We have shown that peach PpeS6PDH gene is down-regulated in flower buds after dormancy release, concomitantly with changes in the methylation level at specific lysine residues of histone H3 (H3K27 and H3K4) in the chromatin around the translation start site of the gene. PpeS6PDH encodes a NADPH-dependent sorbitol-6-phosphate dehydrogenase, the key enzyme for biosynthesis of sorbitol. Consistently, sorbitol accumulates in dormant buds showing higher PpeS6PDH expression. Moreover, PpeS6PDH gene expression is affected by cold and water deficit stress. Particularly, its expression is up-regulated by low temperature in buds and leaves, whereas desiccation treatment induces PpeS6PDH in buds and represses the gene in leaves. These data reveal the concurrent participation of chromatin modification mechanisms, transcriptional regulation of PpeS6PDH and sorbitol accumulation in flower buds of peach. In addition to its role as a major translocatable photosynthate in Rosaceae species, sorbitol is a widespread compatible solute and cryoprotectant, which suggests its participation in tolerance to environmental stresses in flower buds of peach. 2017-11-13T15:30:40Z 2017-11-13T15:30:40Z 2017 article Published version acceptedVersion Lloret, A., Martínez-Fuentes, A., Agustí, M., Badenes, M. L. & Ríos, G. (2017). Chromatin-associated regulation of sorbitol synthesis in flower buds of peach. Plant Molecular Biology, 95, 507-517. 0167-4412 http://hdl.handle.net/20.500.11939/5736 10.1007/s11103-017-0669-6 https://link.springer.com/article/10.1007/s11103-017-0669-6 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Springer electronico |
| spellingShingle | Abiotic stress Bud dormancy Chromatin Prunus persica Sorbitol-6-phosphate dehydrogenase (S6PDH) Aldose-6-phosphate reductase (Ald6PRase) F30 Plant genetics and breeding Prunus persica Bud dormancy Abiotic stress Chromatin Lloret, Alba Martínez-Fuentes, Amparo Agustí, Manuel Badenes, María L. Ríos, Gabino Chromatin-associated regulation of sorbitol synthesis in flower buds of peach |
| title | Chromatin-associated regulation of sorbitol synthesis in flower buds of peach |
| title_full | Chromatin-associated regulation of sorbitol synthesis in flower buds of peach |
| title_fullStr | Chromatin-associated regulation of sorbitol synthesis in flower buds of peach |
| title_full_unstemmed | Chromatin-associated regulation of sorbitol synthesis in flower buds of peach |
| title_short | Chromatin-associated regulation of sorbitol synthesis in flower buds of peach |
| title_sort | chromatin associated regulation of sorbitol synthesis in flower buds of peach |
| topic | Abiotic stress Bud dormancy Chromatin Prunus persica Sorbitol-6-phosphate dehydrogenase (S6PDH) Aldose-6-phosphate reductase (Ald6PRase) F30 Plant genetics and breeding Prunus persica Bud dormancy Abiotic stress Chromatin |
| url | http://hdl.handle.net/20.500.11939/5736 https://link.springer.com/article/10.1007/s11103-017-0669-6 |
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