Estimation of vector propensity for Lettuce mosaic virus based on viral detection in single aphids
Lettuce mosaic virus (LMV) is transmitted by aphids nonpersistently causing severe disease outbreaks in commercial lettuce crops. New strategies to control plant viruses have arisen based on molecular techniques, which analyze plantvirus-vector interactions. In this work, two PCR-based methods with...
| Autores principales: | , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
|
| Acceso en línea: | http://hdl.handle.net/20.500.11939/5689 |
| _version_ | 1855032318534615040 |
|---|---|
| author | Moreno, A. Bertolini, Edson Olmos, Antonio Cambra, Mariano Fereres, Alberto |
| author_browse | Bertolini, Edson Cambra, Mariano Fereres, Alberto Moreno, A. Olmos, Antonio |
| author_facet | Moreno, A. Bertolini, Edson Olmos, Antonio Cambra, Mariano Fereres, Alberto |
| author_sort | Moreno, A. |
| collection | ReDivia |
| description | Lettuce mosaic virus (LMV) is transmitted by aphids nonpersistently causing severe disease outbreaks in commercial lettuce crops. New strategies to control plant viruses have arisen based on molecular techniques, which analyze plantvirus-vector interactions. In this work, two PCR-based methods with a previous immunocapture phase, have been developed to detect LMV in single aphids. Detection rates using a RT-nested-PCR method in single aphids and transmission efficiency of Myzus persicae (vector species) and Nasonovia ribisnigri (nonvector species) were compared. Although the percentage of viruliferous aphids for N. ribisnigri (45.8 +/- 2.3) was higher than for M. persicae (39.2 +/- 3.5) after the same acquisition access period, N. ribisnigri was unable to transmit the virus while M. persicae proved to be an efficient vector (with a transmission rate per single aphid of 10.4 +/- 0.8). A method was proposed to estimate vector propensity for nonpersistent viruses based on the relationship between the percentage of viruliferous aphids and their transmission ability. This methodology could be applied to decision-making and implementing control strategies to prevent virus spreading. |
| format | article |
| id | ReDivia5689 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia56892025-04-25T14:44:35Z Estimation of vector propensity for Lettuce mosaic virus based on viral detection in single aphids Moreno, A. Bertolini, Edson Olmos, Antonio Cambra, Mariano Fereres, Alberto Lettuce mosaic virus (LMV) is transmitted by aphids nonpersistently causing severe disease outbreaks in commercial lettuce crops. New strategies to control plant viruses have arisen based on molecular techniques, which analyze plantvirus-vector interactions. In this work, two PCR-based methods with a previous immunocapture phase, have been developed to detect LMV in single aphids. Detection rates using a RT-nested-PCR method in single aphids and transmission efficiency of Myzus persicae (vector species) and Nasonovia ribisnigri (nonvector species) were compared. Although the percentage of viruliferous aphids for N. ribisnigri (45.8 +/- 2.3) was higher than for M. persicae (39.2 +/- 3.5) after the same acquisition access period, N. ribisnigri was unable to transmit the virus while M. persicae proved to be an efficient vector (with a transmission rate per single aphid of 10.4 +/- 0.8). A method was proposed to estimate vector propensity for nonpersistent viruses based on the relationship between the percentage of viruliferous aphids and their transmission ability. This methodology could be applied to decision-making and implementing control strategies to prevent virus spreading. 2017-06-01T10:12:50Z 2017-06-01T10:12:50Z 2007 SEP 2007 article publishedVersion Moreno, A., Bertolini, E., Olmos, A., Cambra, M., Fereres, A. (2007). Estimation of vector propensity for Lettuce mosaic virus based on viral detection in single aphids. Spanish Journal of Agricultural Research, 5(3), 376-384. 1695-971X http://hdl.handle.net/20.500.11939/5689 en openAccess Impreso |
| spellingShingle | Moreno, A. Bertolini, Edson Olmos, Antonio Cambra, Mariano Fereres, Alberto Estimation of vector propensity for Lettuce mosaic virus based on viral detection in single aphids |
| title | Estimation of vector propensity for Lettuce mosaic virus based on viral detection in single aphids |
| title_full | Estimation of vector propensity for Lettuce mosaic virus based on viral detection in single aphids |
| title_fullStr | Estimation of vector propensity for Lettuce mosaic virus based on viral detection in single aphids |
| title_full_unstemmed | Estimation of vector propensity for Lettuce mosaic virus based on viral detection in single aphids |
| title_short | Estimation of vector propensity for Lettuce mosaic virus based on viral detection in single aphids |
| title_sort | estimation of vector propensity for lettuce mosaic virus based on viral detection in single aphids |
| url | http://hdl.handle.net/20.500.11939/5689 |
| work_keys_str_mv | AT morenoa estimationofvectorpropensityforlettucemosaicvirusbasedonviraldetectioninsingleaphids AT bertoliniedson estimationofvectorpropensityforlettucemosaicvirusbasedonviraldetectioninsingleaphids AT olmosantonio estimationofvectorpropensityforlettucemosaicvirusbasedonviraldetectioninsingleaphids AT cambramariano estimationofvectorpropensityforlettucemosaicvirusbasedonviraldetectioninsingleaphids AT fereresalberto estimationofvectorpropensityforlettucemosaicvirusbasedonviraldetectioninsingleaphids |