Selection of Ceratitis capitata (Diptera: Tephritidae) Specific Recombinant Monoclonal Phage Display Antibodies for Prey Detection Analysis
Several recombinant antibodies against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), one of the most important pests in agriculture worldwide, were selected for the first time from a commercial phage display library of human scFv antibodies. The specificity and...
| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5685 |
| _version_ | 1855032317998792704 |
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| author | Monzó, César Urbaneja, Alberto Ximenez-Embun, Miguel Garcia-Fernandez, Julia García, José L. Castanera, Pedro |
| author_browse | Castanera, Pedro Garcia-Fernandez, Julia García, José L. Monzó, César Urbaneja, Alberto Ximenez-Embun, Miguel |
| author_facet | Monzó, César Urbaneja, Alberto Ximenez-Embun, Miguel Garcia-Fernandez, Julia García, José L. Castanera, Pedro |
| author_sort | Monzó, César |
| collection | ReDivia |
| description | Several recombinant antibodies against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), one of the most important pests in agriculture worldwide, were selected for the first time from a commercial phage display library of human scFv antibodies. The specificity and sensitivity of the selected recombinant antibodies were compared with that of a rabbit polyclonal serum raised in parallel using a wide range of arthropod species as controls. The selected recombinant monoclonal antibodies had a similar or greater specificity when compared with classical monoclonal antibodies. The selected recombinant antibodies were successfully used to detect the target antigen in the gut of predators and the scFv antibodies were sequenced and compared. These results demonstrate the potential for recombinant scFv antibodies to be used as an alternative to the classical monoclonal antibodies or even molecular probes in the post-mortem analysis studies of generalist predators. Citation: Monzo C, Urbaneja A, Ximenez-Embun M, Garcia-Fernandez J, Garcia JL, et al. (2012) Selection of Ceratitis capitata (Diptera: Tephritidae) Specific Recombinant Monoclonal Phage Display Antibodies for Prey Detection Analysis. PLoS ONE 7(12): e51440. doi:10.1371/journal.pone.0051440 |
| format | article |
| id | ReDivia5685 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia56852025-04-25T14:44:35Z Selection of Ceratitis capitata (Diptera: Tephritidae) Specific Recombinant Monoclonal Phage Display Antibodies for Prey Detection Analysis Monzó, César Urbaneja, Alberto Ximenez-Embun, Miguel Garcia-Fernandez, Julia García, José L. Castanera, Pedro Several recombinant antibodies against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), one of the most important pests in agriculture worldwide, were selected for the first time from a commercial phage display library of human scFv antibodies. The specificity and sensitivity of the selected recombinant antibodies were compared with that of a rabbit polyclonal serum raised in parallel using a wide range of arthropod species as controls. The selected recombinant monoclonal antibodies had a similar or greater specificity when compared with classical monoclonal antibodies. The selected recombinant antibodies were successfully used to detect the target antigen in the gut of predators and the scFv antibodies were sequenced and compared. These results demonstrate the potential for recombinant scFv antibodies to be used as an alternative to the classical monoclonal antibodies or even molecular probes in the post-mortem analysis studies of generalist predators. Citation: Monzo C, Urbaneja A, Ximenez-Embun M, Garcia-Fernandez J, Garcia JL, et al. (2012) Selection of Ceratitis capitata (Diptera: Tephritidae) Specific Recombinant Monoclonal Phage Display Antibodies for Prey Detection Analysis. PLoS ONE 7(12): e51440. doi:10.1371/journal.pone.0051440 2017-06-01T10:12:50Z 2017-06-01T10:12:50Z 2012 DEC 14 2012 article publishedVersion Monzó, C., Urbaneja, A., Ximénez-Embún, M., García-Fernández, J., García, J. L., & Castañera, P. (2012). Selection of Ceratitis capitata (Diptera: Tephritidae) specific recombinant monoclonal phage display antibodies for prey detection analysis. PloS one, 7(12), e51440. 1932-6203 http://hdl.handle.net/20.500.11939/5685 10.1371/journal.pone.0051440 en openAccess Impreso |
| spellingShingle | Monzó, César Urbaneja, Alberto Ximenez-Embun, Miguel Garcia-Fernandez, Julia García, José L. Castanera, Pedro Selection of Ceratitis capitata (Diptera: Tephritidae) Specific Recombinant Monoclonal Phage Display Antibodies for Prey Detection Analysis |
| title | Selection of Ceratitis capitata (Diptera: Tephritidae) Specific Recombinant Monoclonal Phage Display Antibodies for Prey Detection Analysis |
| title_full | Selection of Ceratitis capitata (Diptera: Tephritidae) Specific Recombinant Monoclonal Phage Display Antibodies for Prey Detection Analysis |
| title_fullStr | Selection of Ceratitis capitata (Diptera: Tephritidae) Specific Recombinant Monoclonal Phage Display Antibodies for Prey Detection Analysis |
| title_full_unstemmed | Selection of Ceratitis capitata (Diptera: Tephritidae) Specific Recombinant Monoclonal Phage Display Antibodies for Prey Detection Analysis |
| title_short | Selection of Ceratitis capitata (Diptera: Tephritidae) Specific Recombinant Monoclonal Phage Display Antibodies for Prey Detection Analysis |
| title_sort | selection of ceratitis capitata diptera tephritidae specific recombinant monoclonal phage display antibodies for prey detection analysis |
| url | http://hdl.handle.net/20.500.11939/5685 |
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