Cryoprotectant and freezing-process alter the ability of chicken sperm to acrosome react
Sperm surviving after freezing-thawing is usually 40-50% of the initial population. Damage during this process affects both fertilizing ability and its duration in avian species. However, the effect of cryopreservation on the sperm ability to undergo the acrosome reaction, the initial event of ferti...
| Main Authors: | , , |
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| Format: | article |
| Language: | Inglés |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.11939/5646 |
| _version_ | 1855032311889788928 |
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| author | Mocé, Eva Grasseau, I. Blesbois, E. |
| author_browse | Blesbois, E. Grasseau, I. Mocé, Eva |
| author_facet | Mocé, Eva Grasseau, I. Blesbois, E. |
| author_sort | Mocé, Eva |
| collection | ReDivia |
| description | Sperm surviving after freezing-thawing is usually 40-50% of the initial population. Damage during this process affects both fertilizing ability and its duration in avian species. However, the effect of cryopreservation on the sperm ability to undergo the acrosome reaction, the initial event of fertilization, is still in question in birds. In this paper, the influence of cryoprotectant (glycerol and dimethylacetamide-DMA) and of two different cryopreservation processes (pellets or straws) on the ability of rooster sperm to acrosome react (AR measured with PNA-FITC) was studied. Motility parameters (CASA) and plasma membrane integrity (propidium iodide exclusion) were also measured. The addition of cryoprotectants (CPA) immediately provoked a dramatic decrease in the ability of sperm to undergo the acrosome reaction, glycerol being more harmful than DMA. The cryoprotectant removal was also harmful. The other parts of the freezing process further decreased the ability to AR. Motility was affected to a lesser extent by CPA presence although plasma membrane integrity was not altered. The DMA/rapid freezing procedure was the most harmful for plasma membrane integrity. Taken together, these results show that AR is more dramatically altered by CPA presence than motility and membrane integrity and CPA provokes a more pronounced effect than the freezing-thawing process especially in the case of using glycerol/slow freezing process. (C) 2010 Elsevier B.V. All rights reserved. |
| format | article |
| id | ReDivia5646 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia56462025-04-25T14:44:31Z Cryoprotectant and freezing-process alter the ability of chicken sperm to acrosome react Mocé, Eva Grasseau, I. Blesbois, E. Sperm surviving after freezing-thawing is usually 40-50% of the initial population. Damage during this process affects both fertilizing ability and its duration in avian species. However, the effect of cryopreservation on the sperm ability to undergo the acrosome reaction, the initial event of fertilization, is still in question in birds. In this paper, the influence of cryoprotectant (glycerol and dimethylacetamide-DMA) and of two different cryopreservation processes (pellets or straws) on the ability of rooster sperm to acrosome react (AR measured with PNA-FITC) was studied. Motility parameters (CASA) and plasma membrane integrity (propidium iodide exclusion) were also measured. The addition of cryoprotectants (CPA) immediately provoked a dramatic decrease in the ability of sperm to undergo the acrosome reaction, glycerol being more harmful than DMA. The cryoprotectant removal was also harmful. The other parts of the freezing process further decreased the ability to AR. Motility was affected to a lesser extent by CPA presence although plasma membrane integrity was not altered. The DMA/rapid freezing procedure was the most harmful for plasma membrane integrity. Taken together, these results show that AR is more dramatically altered by CPA presence than motility and membrane integrity and CPA provokes a more pronounced effect than the freezing-thawing process especially in the case of using glycerol/slow freezing process. (C) 2010 Elsevier B.V. All rights reserved. 2017-06-01T10:12:44Z 2017-06-01T10:12:44Z 2010 DEC 2010 article Moce, E., Grasseau, I., Blesbois, E. (2010). Cryoprotectant and freezing-process alter the ability of chicken sperm to acrosome react. Animal Reproduction Science, 122(3-4), 359-366. 0378-4320 http://hdl.handle.net/20.500.11939/5646 10.1016/j.anireprosci.2010.10.010 en openAccess Impreso |
| spellingShingle | Mocé, Eva Grasseau, I. Blesbois, E. Cryoprotectant and freezing-process alter the ability of chicken sperm to acrosome react |
| title | Cryoprotectant and freezing-process alter the ability of chicken sperm to acrosome react |
| title_full | Cryoprotectant and freezing-process alter the ability of chicken sperm to acrosome react |
| title_fullStr | Cryoprotectant and freezing-process alter the ability of chicken sperm to acrosome react |
| title_full_unstemmed | Cryoprotectant and freezing-process alter the ability of chicken sperm to acrosome react |
| title_short | Cryoprotectant and freezing-process alter the ability of chicken sperm to acrosome react |
| title_sort | cryoprotectant and freezing process alter the ability of chicken sperm to acrosome react |
| url | http://hdl.handle.net/20.500.11939/5646 |
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