Development and characterization of microsatellite markers in pomegranate (Punica granatum L.)

The pomegranate (Punica granatum L.) is a temperate climate species requiring high temperatures for proper and complete ripening. The species is consumed as a fresh fruit, but also can be used to obtain transformed products such as juice, jam, or preserve. It is a fruit tree species with a high degr...

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Autores principales: Soriano, J. M., Zuriaga, Elena, Rubio, Pia, Llácer, Gerardo, Infante, Rodrigo, Badenes, María L.
Formato: Artículo
Lenguaje:Inglés
Publicado: 2017
Acceso en línea:http://hdl.handle.net/20.500.11939/5629
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author Soriano, J. M.
Zuriaga, Elena
Rubio, Pia
Llácer, Gerardo
Infante, Rodrigo
Badenes, María L.
author_browse Badenes, María L.
Infante, Rodrigo
Llácer, Gerardo
Rubio, Pia
Soriano, J. M.
Zuriaga, Elena
author_facet Soriano, J. M.
Zuriaga, Elena
Rubio, Pia
Llácer, Gerardo
Infante, Rodrigo
Badenes, María L.
author_sort Soriano, J. M.
collection ReDivia
description The pomegranate (Punica granatum L.) is a temperate climate species requiring high temperatures for proper and complete ripening. The species is consumed as a fresh fruit, but also can be used to obtain transformed products such as juice, jam, or preserve. It is a fruit tree species with a high degree of diversity, but the identification of cultivars by morphological traits is very difficult. Thus, the characterization of genotypes through molecular markers is of great value for germplasm preservation, genetic studies, and plant breeding. The number of simple sequence repeat (SSR or microsatellite) markers developed for this genus is too low, so in this work we report the development of 117 microsatellite loci from a CT/AG-enriched pomegranate genomic library. In order to check their utility, eleven accessions were analyzed. The polymorphism information content (PIC) value across all loci ranged between 0.09 and 0.71, with an average of 0.37. These markers will facilitate genetic diversity studies, mapping, and genotyping of pomegranate.
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spelling ReDivia56292025-04-25T14:44:29Z Development and characterization of microsatellite markers in pomegranate (Punica granatum L.) Soriano, J. M. Zuriaga, Elena Rubio, Pia Llácer, Gerardo Infante, Rodrigo Badenes, María L. The pomegranate (Punica granatum L.) is a temperate climate species requiring high temperatures for proper and complete ripening. The species is consumed as a fresh fruit, but also can be used to obtain transformed products such as juice, jam, or preserve. It is a fruit tree species with a high degree of diversity, but the identification of cultivars by morphological traits is very difficult. Thus, the characterization of genotypes through molecular markers is of great value for germplasm preservation, genetic studies, and plant breeding. The number of simple sequence repeat (SSR or microsatellite) markers developed for this genus is too low, so in this work we report the development of 117 microsatellite loci from a CT/AG-enriched pomegranate genomic library. In order to check their utility, eleven accessions were analyzed. The polymorphism information content (PIC) value across all loci ranged between 0.09 and 0.71, with an average of 0.37. These markers will facilitate genetic diversity studies, mapping, and genotyping of pomegranate. 2017-06-01T10:12:42Z 2017-06-01T10:12:42Z 2011 JAN 2011 article Soriano, J. M., Zuriaga, E., Rubio, P., Llacer, G., Infante, R. & Badenes, M. L. (2011). Development and characterization of microsatellite markers in pomegranate (Punica granatum L.). Molecular Breeding, 27(1), 119-128. 1380-3743 http://hdl.handle.net/20.500.11939/5629 10.1007/s11032-010-9511-4 en openAccess Impreso
spellingShingle Soriano, J. M.
Zuriaga, Elena
Rubio, Pia
Llácer, Gerardo
Infante, Rodrigo
Badenes, María L.
Development and characterization of microsatellite markers in pomegranate (Punica granatum L.)
title Development and characterization of microsatellite markers in pomegranate (Punica granatum L.)
title_full Development and characterization of microsatellite markers in pomegranate (Punica granatum L.)
title_fullStr Development and characterization of microsatellite markers in pomegranate (Punica granatum L.)
title_full_unstemmed Development and characterization of microsatellite markers in pomegranate (Punica granatum L.)
title_short Development and characterization of microsatellite markers in pomegranate (Punica granatum L.)
title_sort development and characterization of microsatellite markers in pomegranate punica granatum l
url http://hdl.handle.net/20.500.11939/5629
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