Molecular markers flanking citrus tristeza virus resistance gene from Poncirus trifoliata (L) Raf
Two segregating populations for citrus tristeza virus (CTV) resistance derived from Poncirus trifoliata var 'Flying Dragon' by self-pollination and pollination to Citrus medica L. var ethrog 'Arizona' were inoculated with a common CTV isolate. The presence of virus was checked by the...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5626 |
| _version_ | 1855491956600209408 |
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| author | Mestre, P. F. Asins, María J. Pina, José A. Carbonell, Emilio A. Navarro, Luis |
| author_browse | Asins, María J. Carbonell, Emilio A. Mestre, P. F. Navarro, Luis Pina, José A. |
| author_facet | Mestre, P. F. Asins, María J. Pina, José A. Carbonell, Emilio A. Navarro, Luis |
| author_sort | Mestre, P. F. |
| collection | ReDivia |
| description | Two segregating populations for citrus tristeza virus (CTV) resistance derived from Poncirus trifoliata var 'Flying Dragon' by self-pollination and pollination to Citrus medica L. var ethrog 'Arizona' were inoculated with a common CTV isolate. The presence of virus was checked by the Double Antibody Sandwich Enzyme-Linked Assay and Direct Tissue Blot Inmunoassay at 3, 6, and 12 months after inoculation. Seven RAPDs were found linked to the CTV resistance gene by bulked segregant analysis. The closest linked RAPDs were cloned to obtain linked codominant RFLPs and to increase the precision of the tic distance estimation. The CTV resistance gene seems to be located between cW18 and cK16. Differences in genetic distances among progenies are large and can be explained by genome-wide reduction in the recombination of progeny derived from male versus female gametes. |
| format | Artículo |
| id | ReDivia5626 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia56262025-04-25T14:44:29Z Molecular markers flanking citrus tristeza virus resistance gene from Poncirus trifoliata (L) Raf Mestre, P. F. Asins, María J. Pina, José A. Carbonell, Emilio A. Navarro, Luis Two segregating populations for citrus tristeza virus (CTV) resistance derived from Poncirus trifoliata var 'Flying Dragon' by self-pollination and pollination to Citrus medica L. var ethrog 'Arizona' were inoculated with a common CTV isolate. The presence of virus was checked by the Double Antibody Sandwich Enzyme-Linked Assay and Direct Tissue Blot Inmunoassay at 3, 6, and 12 months after inoculation. Seven RAPDs were found linked to the CTV resistance gene by bulked segregant analysis. The closest linked RAPDs were cloned to obtain linked codominant RFLPs and to increase the precision of the tic distance estimation. The CTV resistance gene seems to be located between cW18 and cK16. Differences in genetic distances among progenies are large and can be explained by genome-wide reduction in the recombination of progeny derived from male versus female gametes. 2017-06-01T10:12:41Z 2017-06-01T10:12:41Z 1997 MAR article Mestre, PF, Asins, M.J., Pina, JA, Carbonell, EA, Navarro, L. (1997). Molecular markers flanking citrus tristeza virus resistance gene from Poncirus trifoliata (L), Raf. Theoretical and Applied Genetics, 94(3-4), 458-464. 0040-5752 http://hdl.handle.net/20.500.11939/5626 10.1007/s001220050437 en openAccess Impreso |
| spellingShingle | Mestre, P. F. Asins, María J. Pina, José A. Carbonell, Emilio A. Navarro, Luis Molecular markers flanking citrus tristeza virus resistance gene from Poncirus trifoliata (L) Raf |
| title | Molecular markers flanking citrus tristeza virus resistance gene from Poncirus trifoliata (L) Raf |
| title_full | Molecular markers flanking citrus tristeza virus resistance gene from Poncirus trifoliata (L) Raf |
| title_fullStr | Molecular markers flanking citrus tristeza virus resistance gene from Poncirus trifoliata (L) Raf |
| title_full_unstemmed | Molecular markers flanking citrus tristeza virus resistance gene from Poncirus trifoliata (L) Raf |
| title_short | Molecular markers flanking citrus tristeza virus resistance gene from Poncirus trifoliata (L) Raf |
| title_sort | molecular markers flanking citrus tristeza virus resistance gene from poncirus trifoliata l raf |
| url | http://hdl.handle.net/20.500.11939/5626 |
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