Pseudomonas savastanoi pv. savastanoi Contains Two iaaL Paralogs, One of Which Exhibits a Variable Number of a Trinucleotide (TAC) Tandem Repeat
In this study, Pseudomonas savastanoi pv. savastanoi isolates were demonstrated to contain two iaaL paralogs, which are both chromosomally located in most strains. Comparative analysis of iaaL nucleotide sequences amplified from these two paralogs revealed that one paralog, iaaL(Ps)n, is 100% identi...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5614 |
| _version_ | 1855491954642518016 |
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| author | Matas, Isabel M. Perez-Martinez, Isabel Quesada, Jose M. Rodriguez-Herva, Jose J. Penyalver, Ramón Ramos, Cayo |
| author_browse | Matas, Isabel M. Penyalver, Ramón Perez-Martinez, Isabel Quesada, Jose M. Ramos, Cayo Rodriguez-Herva, Jose J. |
| author_facet | Matas, Isabel M. Perez-Martinez, Isabel Quesada, Jose M. Rodriguez-Herva, Jose J. Penyalver, Ramón Ramos, Cayo |
| author_sort | Matas, Isabel M. |
| collection | ReDivia |
| description | In this study, Pseudomonas savastanoi pv. savastanoi isolates were demonstrated to contain two iaaL paralogs, which are both chromosomally located in most strains. Comparative analysis of iaaL nucleotide sequences amplified from these two paralogs revealed that one paralog, iaaL(Ps)n, is 100% identical to iaaL from P. savastanoi pv. nerii, while the other paralog, iaaL(Psv), exhibited 93% identity to iaaL from Pseudomonas syringae pv. tomato (iaaL(Pto)). A 3-nucleotide motif (TAC) comprised of 3 to 15 repeats, which remained stable after propagation of the strains in olive plants, was found in iaaL(Psv). Based on the observed nucleotide sequence variations, a restriction fragment length polymorphism assay was developed that allowed differentiation among iaaL(Psn), iaaL(Psv), and iaaL(Pto). In addition, reverse transcriptase PCR on total RNA from P. savastanoi pv. savastanoi strains demonstrated that both iaaL(Psv) and iaaL(Psn) containing 14 or fewer TAC repeats are transcribed. Capillary electrophoresis analysis of PCR-amplified DNA fragments containing the TAC repeats from iaaL(Psv) allowed the differentiation of P. savastanoi pv. savastanoi isolates. |
| format | Artículo |
| id | ReDivia5614 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia56142025-04-25T14:43:26Z Pseudomonas savastanoi pv. savastanoi Contains Two iaaL Paralogs, One of Which Exhibits a Variable Number of a Trinucleotide (TAC) Tandem Repeat Matas, Isabel M. Perez-Martinez, Isabel Quesada, Jose M. Rodriguez-Herva, Jose J. Penyalver, Ramón Ramos, Cayo In this study, Pseudomonas savastanoi pv. savastanoi isolates were demonstrated to contain two iaaL paralogs, which are both chromosomally located in most strains. Comparative analysis of iaaL nucleotide sequences amplified from these two paralogs revealed that one paralog, iaaL(Ps)n, is 100% identical to iaaL from P. savastanoi pv. nerii, while the other paralog, iaaL(Psv), exhibited 93% identity to iaaL from Pseudomonas syringae pv. tomato (iaaL(Pto)). A 3-nucleotide motif (TAC) comprised of 3 to 15 repeats, which remained stable after propagation of the strains in olive plants, was found in iaaL(Psv). Based on the observed nucleotide sequence variations, a restriction fragment length polymorphism assay was developed that allowed differentiation among iaaL(Psn), iaaL(Psv), and iaaL(Pto). In addition, reverse transcriptase PCR on total RNA from P. savastanoi pv. savastanoi strains demonstrated that both iaaL(Psv) and iaaL(Psn) containing 14 or fewer TAC repeats are transcribed. Capillary electrophoresis analysis of PCR-amplified DNA fragments containing the TAC repeats from iaaL(Psv) allowed the differentiation of P. savastanoi pv. savastanoi isolates. 2017-06-01T10:12:40Z 2017-06-01T10:12:40Z 2009 FEB 15 2009 article Matas, I.M., Perez-Martinez, I., Quesada, J.M., Rodriguez-Herva, J.J., Penyalver, R., Ramos, C. (2009). Pseudomonas savastanoi pv. savastanoi Contains Two iaaL Paralogs, One of Which Exhibits a Variable Number of a Trinucleotide (TAC), Tandem Repeat. Applied and Environmental Microbiology, 75(4), 1030-1035. 0099-2240 http://hdl.handle.net/20.500.11939/5614 10.1128/AEM.01572-08 en openAccess Impreso |
| spellingShingle | Matas, Isabel M. Perez-Martinez, Isabel Quesada, Jose M. Rodriguez-Herva, Jose J. Penyalver, Ramón Ramos, Cayo Pseudomonas savastanoi pv. savastanoi Contains Two iaaL Paralogs, One of Which Exhibits a Variable Number of a Trinucleotide (TAC) Tandem Repeat |
| title | Pseudomonas savastanoi pv. savastanoi Contains Two iaaL Paralogs, One of Which Exhibits a Variable Number of a Trinucleotide (TAC) Tandem Repeat |
| title_full | Pseudomonas savastanoi pv. savastanoi Contains Two iaaL Paralogs, One of Which Exhibits a Variable Number of a Trinucleotide (TAC) Tandem Repeat |
| title_fullStr | Pseudomonas savastanoi pv. savastanoi Contains Two iaaL Paralogs, One of Which Exhibits a Variable Number of a Trinucleotide (TAC) Tandem Repeat |
| title_full_unstemmed | Pseudomonas savastanoi pv. savastanoi Contains Two iaaL Paralogs, One of Which Exhibits a Variable Number of a Trinucleotide (TAC) Tandem Repeat |
| title_short | Pseudomonas savastanoi pv. savastanoi Contains Two iaaL Paralogs, One of Which Exhibits a Variable Number of a Trinucleotide (TAC) Tandem Repeat |
| title_sort | pseudomonas savastanoi pv savastanoi contains two iaal paralogs one of which exhibits a variable number of a trinucleotide tac tandem repeat |
| url | http://hdl.handle.net/20.500.11939/5614 |
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