Effects of high levels of zinc and manganese ions on Strategy I responses to iron deficiency in citrus
The aim of this work is to study the effect of elevated concentrations of either Zn2+ or Mn2+ in the culture media on the components of the system of iron acquisition by roots from Fe-deficient and Fe-supplied citrus seedlings. Seeds of Citrus aurantium (L.) were germinated in vitro. Nutrient soluti...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5601 |
| _version_ | 1855491952480354304 |
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| author | Martínez-Cuenca, Mary-Rus Quinones, Ana Iglesias, Domingo J. Forner-Giner, María A. Primo-Millo, Eduardo Legaz, Francisco |
| author_browse | Forner-Giner, María A. Iglesias, Domingo J. Legaz, Francisco Martínez-Cuenca, Mary-Rus Primo-Millo, Eduardo Quinones, Ana |
| author_facet | Martínez-Cuenca, Mary-Rus Quinones, Ana Iglesias, Domingo J. Forner-Giner, María A. Primo-Millo, Eduardo Legaz, Francisco |
| author_sort | Martínez-Cuenca, Mary-Rus |
| collection | ReDivia |
| description | The aim of this work is to study the effect of elevated concentrations of either Zn2+ or Mn2+ in the culture media on the components of the system of iron acquisition by roots from Fe-deficient and Fe-supplied citrus seedlings. Seeds of Citrus aurantium (L.) were germinated in vitro. Nutrient solutions without iron, zinc or manganese were prepared for assays and then combinations of Fe-EDDHA, ZnSO4 center dot 7H(2)O or MnSO4 center dot H2O were added to obtain the following media: -Fe-Zn, +Fe-Zn, -Fe+Zn, +Fe+Zn, -Fe-Mn, +Fe-Mn, -Fe+Mn and +Fe+Mn. Data indicated that when either 100 mu M Zn2+ or 250 mu M Mn2+ were added to culture media containing 20 mu M Fe, iron concentration in roots and shoots was reduced with respect to the seedlings cultured in the same medium lacking Zn2+ or Mn2+. Reciprocally, the presence of iron in the medium lowered Zn and Mn levels in the plant. Addition of either 100 mu M Zn2+ or 250 mu M Mn2+ to the medium enhanced the transcription levels of FRO2, IRT1 and NRAMP3 genes in roots, this effect being more marked in Fe-starved plants. FC-R activity followed the same behaviour as the gene FRO2. Both Zn2+ and Mn2+ stimulated the activity of some genes (FRO2, IRT1 and NRAMP3) which was also induced by Fe-deficiency, although these effects appear to be independent of the inhibition of Fe uptake by these cations. |
| format | Artículo |
| id | ReDivia5601 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia56012025-04-25T14:43:23Z Effects of high levels of zinc and manganese ions on Strategy I responses to iron deficiency in citrus Martínez-Cuenca, Mary-Rus Quinones, Ana Iglesias, Domingo J. Forner-Giner, María A. Primo-Millo, Eduardo Legaz, Francisco The aim of this work is to study the effect of elevated concentrations of either Zn2+ or Mn2+ in the culture media on the components of the system of iron acquisition by roots from Fe-deficient and Fe-supplied citrus seedlings. Seeds of Citrus aurantium (L.) were germinated in vitro. Nutrient solutions without iron, zinc or manganese were prepared for assays and then combinations of Fe-EDDHA, ZnSO4 center dot 7H(2)O or MnSO4 center dot H2O were added to obtain the following media: -Fe-Zn, +Fe-Zn, -Fe+Zn, +Fe+Zn, -Fe-Mn, +Fe-Mn, -Fe+Mn and +Fe+Mn. Data indicated that when either 100 mu M Zn2+ or 250 mu M Mn2+ were added to culture media containing 20 mu M Fe, iron concentration in roots and shoots was reduced with respect to the seedlings cultured in the same medium lacking Zn2+ or Mn2+. Reciprocally, the presence of iron in the medium lowered Zn and Mn levels in the plant. Addition of either 100 mu M Zn2+ or 250 mu M Mn2+ to the medium enhanced the transcription levels of FRO2, IRT1 and NRAMP3 genes in roots, this effect being more marked in Fe-starved plants. FC-R activity followed the same behaviour as the gene FRO2. Both Zn2+ and Mn2+ stimulated the activity of some genes (FRO2, IRT1 and NRAMP3) which was also induced by Fe-deficiency, although these effects appear to be independent of the inhibition of Fe uptake by these cations. 2017-06-01T10:12:38Z 2017-06-01T10:12:38Z 2013 DEC 2013 article Martinez-Cuenca, M.R., Quinones, A., Iglesias, D.J., Forner-Giner, M.A., Primo-Millo, E., Legaz, F. (2013). Effects of high levels of zinc and manganese ions on Strategy I responses to iron deficiency in citrus. Plant and Soil, 373(1-2), 943-953. 0032-079X; 1573-5036 http://hdl.handle.net/20.500.11939/5601 10.1007/s11104-013-1861-3 en openAccess Impreso |
| spellingShingle | Martínez-Cuenca, Mary-Rus Quinones, Ana Iglesias, Domingo J. Forner-Giner, María A. Primo-Millo, Eduardo Legaz, Francisco Effects of high levels of zinc and manganese ions on Strategy I responses to iron deficiency in citrus |
| title | Effects of high levels of zinc and manganese ions on Strategy I responses to iron deficiency in citrus |
| title_full | Effects of high levels of zinc and manganese ions on Strategy I responses to iron deficiency in citrus |
| title_fullStr | Effects of high levels of zinc and manganese ions on Strategy I responses to iron deficiency in citrus |
| title_full_unstemmed | Effects of high levels of zinc and manganese ions on Strategy I responses to iron deficiency in citrus |
| title_short | Effects of high levels of zinc and manganese ions on Strategy I responses to iron deficiency in citrus |
| title_sort | effects of high levels of zinc and manganese ions on strategy i responses to iron deficiency in citrus |
| url | http://hdl.handle.net/20.500.11939/5601 |
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