Metabolic responses to iron deficiency in roots of Carrizo citrange [Citrus sinensis (L.) Osbeck. x Poncirus trifoliata (L.) Raf.]
The effects of iron (Fe) deficiency on the low-molecular-weight organic acid (LMWOA) metabolism have been investigated in Carrizo citrange (CC) [Citrus sinensis (L.) Osb. x Poncirus trifoliata (L.) Raf.] roots. Major LMWOAs found in roots, xylem sap and root exudates were citrate and malate and thei...
| Autores principales: | , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5599 |
| _version_ | 1855032304625254400 |
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| author | Martínez-Cuenca, Mary-Rus Iglesias, Domingo J. Talón, Manuel Abadia, Javier Lopez-Millan, Ana-Flor Primo-Millo, Eduardo Legaz, Francisco |
| author_browse | Abadia, Javier Iglesias, Domingo J. Legaz, Francisco Lopez-Millan, Ana-Flor Martínez-Cuenca, Mary-Rus Primo-Millo, Eduardo Talón, Manuel |
| author_facet | Martínez-Cuenca, Mary-Rus Iglesias, Domingo J. Talón, Manuel Abadia, Javier Lopez-Millan, Ana-Flor Primo-Millo, Eduardo Legaz, Francisco |
| author_sort | Martínez-Cuenca, Mary-Rus |
| collection | ReDivia |
| description | The effects of iron (Fe) deficiency on the low-molecular-weight organic acid (LMWOA) metabolism have been investigated in Carrizo citrange (CC) [Citrus sinensis (L.) Osb. x Poncirus trifoliata (L.) Raf.] roots. Major LMWOAs found in roots, xylem sap and root exudates were citrate and malate and their concentrations increased with Fe deficiency. The activities of several enzymes involved in the LMWOA metabolism were also assessed in roots. In the cytosolic fraction, the activities of malate dehydrogenase (cMDH) and phosphoenolpyruvate carboxylase (PEPC) enzymes were 132 and 100% higher in Fe-deficient conditions, whereas the activity of pyruvate kinase was 31% lower and the activity of malic enzyme (ME) did not change. In the mitochondrial fraction, the activities of fumarase, MDH and citrate synthase enzymes were 158, 117 and 53% higher, respectively, in Fe-deficient extracts when compared with Fe-sufficient controls, whereas no significant differences between treatments were found for aconitase (ACO) activity. The expression of their corresponding genes in roots of Fe-deficient plants was higher than that measured in Fe-sufficient controls, except for ACO and ME. Also, dicarboxylate-tricarboxylate carrier (DTC) expression was significantly increased in Fe-deficient roots. In conclusion, Fe deficiency in CC seedlings causes a reprogramming of the carbon metabolism that involves an increase of anaplerotic fixation of carbon via PEPC and MDH activities in the cytosol and a shift of the Krebs cycle in the mitochondria towards a non-cyclic mode, as previously described in herbaceous species. In this scheme, DTC could play an important role shuttling both malate and reducing equivalents between the cytosol and the mitochondria. As a result of this metabolic switch malate and citrate concentrations in roots, xylem sap and root exudates increase. |
| format | article |
| id | ReDivia5599 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia55992025-04-25T14:43:23Z Metabolic responses to iron deficiency in roots of Carrizo citrange [Citrus sinensis (L.) Osbeck. x Poncirus trifoliata (L.) Raf.] Martínez-Cuenca, Mary-Rus Iglesias, Domingo J. Talón, Manuel Abadia, Javier Lopez-Millan, Ana-Flor Primo-Millo, Eduardo Legaz, Francisco The effects of iron (Fe) deficiency on the low-molecular-weight organic acid (LMWOA) metabolism have been investigated in Carrizo citrange (CC) [Citrus sinensis (L.) Osb. x Poncirus trifoliata (L.) Raf.] roots. Major LMWOAs found in roots, xylem sap and root exudates were citrate and malate and their concentrations increased with Fe deficiency. The activities of several enzymes involved in the LMWOA metabolism were also assessed in roots. In the cytosolic fraction, the activities of malate dehydrogenase (cMDH) and phosphoenolpyruvate carboxylase (PEPC) enzymes were 132 and 100% higher in Fe-deficient conditions, whereas the activity of pyruvate kinase was 31% lower and the activity of malic enzyme (ME) did not change. In the mitochondrial fraction, the activities of fumarase, MDH and citrate synthase enzymes were 158, 117 and 53% higher, respectively, in Fe-deficient extracts when compared with Fe-sufficient controls, whereas no significant differences between treatments were found for aconitase (ACO) activity. The expression of their corresponding genes in roots of Fe-deficient plants was higher than that measured in Fe-sufficient controls, except for ACO and ME. Also, dicarboxylate-tricarboxylate carrier (DTC) expression was significantly increased in Fe-deficient roots. In conclusion, Fe deficiency in CC seedlings causes a reprogramming of the carbon metabolism that involves an increase of anaplerotic fixation of carbon via PEPC and MDH activities in the cytosol and a shift of the Krebs cycle in the mitochondria towards a non-cyclic mode, as previously described in herbaceous species. In this scheme, DTC could play an important role shuttling both malate and reducing equivalents between the cytosol and the mitochondria. As a result of this metabolic switch malate and citrate concentrations in roots, xylem sap and root exudates increase. 2017-06-01T10:12:38Z 2017-06-01T10:12:38Z 2013 MAR 2013 article Martinez-Cuenca, M.R., Iglesias, D.J., Talón, M., Abadia, J., Lopez-Millan, Ana-Flor, Primo-Millo, E., Legaz, F. (2013). Metabolic responses to iron deficiency in roots of Carrizo citrange [Citrus sinensis (L.), Osbeck. x Poncirus trifoliata (L.), Raf.]. Tree physiology, 33(3), 320-329. 0829-318X http://hdl.handle.net/20.500.11939/5599 10.1093/treephys/tpt011 en openAccess Impreso |
| spellingShingle | Martínez-Cuenca, Mary-Rus Iglesias, Domingo J. Talón, Manuel Abadia, Javier Lopez-Millan, Ana-Flor Primo-Millo, Eduardo Legaz, Francisco Metabolic responses to iron deficiency in roots of Carrizo citrange [Citrus sinensis (L.) Osbeck. x Poncirus trifoliata (L.) Raf.] |
| title | Metabolic responses to iron deficiency in roots of Carrizo citrange [Citrus sinensis (L.) Osbeck. x Poncirus trifoliata (L.) Raf.] |
| title_full | Metabolic responses to iron deficiency in roots of Carrizo citrange [Citrus sinensis (L.) Osbeck. x Poncirus trifoliata (L.) Raf.] |
| title_fullStr | Metabolic responses to iron deficiency in roots of Carrizo citrange [Citrus sinensis (L.) Osbeck. x Poncirus trifoliata (L.) Raf.] |
| title_full_unstemmed | Metabolic responses to iron deficiency in roots of Carrizo citrange [Citrus sinensis (L.) Osbeck. x Poncirus trifoliata (L.) Raf.] |
| title_short | Metabolic responses to iron deficiency in roots of Carrizo citrange [Citrus sinensis (L.) Osbeck. x Poncirus trifoliata (L.) Raf.] |
| title_sort | metabolic responses to iron deficiency in roots of carrizo citrange citrus sinensis l osbeck x poncirus trifoliata l raf |
| url | http://hdl.handle.net/20.500.11939/5599 |
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