Comparative expression of candidate genes involved in sodium transport and compartmentation in citrus
Plants possess a number of mechanisms to cope with sodium (Na+) under salt stress conditions that include minimizing Ne influx, maximizing efflux back to the growth medium or to apoplastic spaces via Na+/H+. antiporters in the plasma membrane, intracellular compartrnentation of Na+ into the vacuole,...
| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5585 |
| _version_ | 1855032302173683712 |
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| author | Martínez-Alcántara, Belén Martínez-Cuenca, Mary-Rus Quinones, Ana Iglesias, Domingo J. Primo-Millo, Eduardo Forner-Giner, María A. |
| author_browse | Forner-Giner, María A. Iglesias, Domingo J. Martínez-Alcántara, Belén Martínez-Cuenca, Mary-Rus Primo-Millo, Eduardo Quinones, Ana |
| author_facet | Martínez-Alcántara, Belén Martínez-Cuenca, Mary-Rus Quinones, Ana Iglesias, Domingo J. Primo-Millo, Eduardo Forner-Giner, María A. |
| author_sort | Martínez-Alcántara, Belén |
| collection | ReDivia |
| description | Plants possess a number of mechanisms to cope with sodium (Na+) under salt stress conditions that include minimizing Ne influx, maximizing efflux back to the growth medium or to apoplastic spaces via Na+/H+. antiporters in the plasma membrane, intracellular compartrnentation of Na+ into the vacuole, as well as recirculation of Na+ out of the shoot via the phloem. Na+ transport in plants constitutes a complex system, in which different Na+ transporters are closely related and their functions are matched tightly. The fact that in citrus under salt stress does chloride appear to be the more toxic ion has led to little attention being paid to Na+ uptake and transport mechanisms in citrus. The aim of this study was to provide insight into the links between the expression levels of candidate Na+ transporter genes (SOS1, NHX1, HKT1), as well as tonoplast proton pumps (V-ATPase, V-PPiase), and Na+ tolerance in two citrus rootstocks, Cleopatra mandarin and trifoliate orange, differing in their Na+ exclusion capacity under salt stress. According to the results of this preliminary study, we hypothesize that higher root Na+ concentration in trifoliate orange genotype, and thus lower allocation of this ion in the shoots, is the result of an enhanced retrieval of Na+ from xylem stream, and an impaired translocation to the shoot tissues, probably as a consequence of the overexpression of putative SOS1 (in roots) and HKT1 (both in roots and shoots). Moreover, the higher transcriptional levels of putative NHX1 found in roots and shoots of trifoliate orange plantlets compared to those of Cleopatra mandarin, together with the enhanced activity of the tonoplast proton pumps in the former, might reveal the preferential sequestration into vacuole of retrieved Na+ from xylem mainly in roots. (C) 2014 Published by Elsevier B.V. |
| format | article |
| id | ReDivia5585 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia55852025-04-25T14:43:21Z Comparative expression of candidate genes involved in sodium transport and compartmentation in citrus Martínez-Alcántara, Belén Martínez-Cuenca, Mary-Rus Quinones, Ana Iglesias, Domingo J. Primo-Millo, Eduardo Forner-Giner, María A. Plants possess a number of mechanisms to cope with sodium (Na+) under salt stress conditions that include minimizing Ne influx, maximizing efflux back to the growth medium or to apoplastic spaces via Na+/H+. antiporters in the plasma membrane, intracellular compartrnentation of Na+ into the vacuole, as well as recirculation of Na+ out of the shoot via the phloem. Na+ transport in plants constitutes a complex system, in which different Na+ transporters are closely related and their functions are matched tightly. The fact that in citrus under salt stress does chloride appear to be the more toxic ion has led to little attention being paid to Na+ uptake and transport mechanisms in citrus. The aim of this study was to provide insight into the links between the expression levels of candidate Na+ transporter genes (SOS1, NHX1, HKT1), as well as tonoplast proton pumps (V-ATPase, V-PPiase), and Na+ tolerance in two citrus rootstocks, Cleopatra mandarin and trifoliate orange, differing in their Na+ exclusion capacity under salt stress. According to the results of this preliminary study, we hypothesize that higher root Na+ concentration in trifoliate orange genotype, and thus lower allocation of this ion in the shoots, is the result of an enhanced retrieval of Na+ from xylem stream, and an impaired translocation to the shoot tissues, probably as a consequence of the overexpression of putative SOS1 (in roots) and HKT1 (both in roots and shoots). Moreover, the higher transcriptional levels of putative NHX1 found in roots and shoots of trifoliate orange plantlets compared to those of Cleopatra mandarin, together with the enhanced activity of the tonoplast proton pumps in the former, might reveal the preferential sequestration into vacuole of retrieved Na+ from xylem mainly in roots. (C) 2014 Published by Elsevier B.V. 2017-06-01T10:12:36Z 2017-06-01T10:12:36Z 2015 MAR 2015 article Martínez-Alcántara, B., Martinez-Cuenca, M.R., Quinones, A., Iglesias, D. J., Primo-Millo, E., Forner-Giner, María A. (2015). Comparative expression of candidate genes involved in sodium transport and compartmentation in citrus. Environmental and experimental botany, 111, 52-62. 0098-8472 http://hdl.handle.net/20.500.11939/5585 10.1016/j.envexpbot.2014.11.002 en openAccess Impreso |
| spellingShingle | Martínez-Alcántara, Belén Martínez-Cuenca, Mary-Rus Quinones, Ana Iglesias, Domingo J. Primo-Millo, Eduardo Forner-Giner, María A. Comparative expression of candidate genes involved in sodium transport and compartmentation in citrus |
| title | Comparative expression of candidate genes involved in sodium transport and compartmentation in citrus |
| title_full | Comparative expression of candidate genes involved in sodium transport and compartmentation in citrus |
| title_fullStr | Comparative expression of candidate genes involved in sodium transport and compartmentation in citrus |
| title_full_unstemmed | Comparative expression of candidate genes involved in sodium transport and compartmentation in citrus |
| title_short | Comparative expression of candidate genes involved in sodium transport and compartmentation in citrus |
| title_sort | comparative expression of candidate genes involved in sodium transport and compartmentation in citrus |
| url | http://hdl.handle.net/20.500.11939/5585 |
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