Extracellular proteases inhibit protein-dependent biofilm formation in Staphylococcus aureus
In Staphylococcus aureus, biofilm formation can be associated with the production of surface-anchored proteins, including Bap, SasG, FnBPs or Spa. By mutational analysis, and using a model strain in which biofilm formation was Bap-dependent, we found that sigma(B) was essential for protein-dependent...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5574 |
| _version_ | 1855491947859279872 |
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| author | Marti, Miguel Trotonda, Maria P. Tormo-Mas, María A. Vergara-Irigaray, Marta Cheung, Ambrose L. Lasa, Inigo Penadés, José R. |
| author_browse | Cheung, Ambrose L. Lasa, Inigo Marti, Miguel Penadés, José R. Tormo-Mas, María A. Trotonda, Maria P. Vergara-Irigaray, Marta |
| author_facet | Marti, Miguel Trotonda, Maria P. Tormo-Mas, María A. Vergara-Irigaray, Marta Cheung, Ambrose L. Lasa, Inigo Penadés, José R. |
| author_sort | Marti, Miguel |
| collection | ReDivia |
| description | In Staphylococcus aureus, biofilm formation can be associated with the production of surface-anchored proteins, including Bap, SasG, FnBPs or Spa. By mutational analysis, and using a model strain in which biofilm formation was Bap-dependent, we found that sigma(B) was essential for protein-dependent biofilm development. Non-polar mutations of sigma(B) in genetically unrelated S. aureus strains lowered the Bap expression and completely impaired biofilm development. Although Northern blot analysis showed a slight reduction in bap, transcription, we demonstrated that Aur and SspA, two proteases that are overexpressed in the sigB mutant strain and are capable of degrading Bap, inhibit biofilm formation. Interestingly, a double sigB-agr mutant, which showed a diminished capacity to express extracellular proteases, was able to restore biofilm formation. Since the vast majority of the S. aureus global regulators control the expression of the extracellular proteases, the results of this work demonstrate the existence of a new pathway controlling protein-mediated biofilm formation in which different global regulators modulate biofilm formation by controlling the expression of S. aureus extracellular proteases. |
| format | Artículo |
| id | ReDivia5574 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia55742025-04-25T14:43:20Z Extracellular proteases inhibit protein-dependent biofilm formation in Staphylococcus aureus Marti, Miguel Trotonda, Maria P. Tormo-Mas, María A. Vergara-Irigaray, Marta Cheung, Ambrose L. Lasa, Inigo Penadés, José R. In Staphylococcus aureus, biofilm formation can be associated with the production of surface-anchored proteins, including Bap, SasG, FnBPs or Spa. By mutational analysis, and using a model strain in which biofilm formation was Bap-dependent, we found that sigma(B) was essential for protein-dependent biofilm development. Non-polar mutations of sigma(B) in genetically unrelated S. aureus strains lowered the Bap expression and completely impaired biofilm development. Although Northern blot analysis showed a slight reduction in bap, transcription, we demonstrated that Aur and SspA, two proteases that are overexpressed in the sigB mutant strain and are capable of degrading Bap, inhibit biofilm formation. Interestingly, a double sigB-agr mutant, which showed a diminished capacity to express extracellular proteases, was able to restore biofilm formation. Since the vast majority of the S. aureus global regulators control the expression of the extracellular proteases, the results of this work demonstrate the existence of a new pathway controlling protein-mediated biofilm formation in which different global regulators modulate biofilm formation by controlling the expression of S. aureus extracellular proteases. 2017-06-01T10:12:35Z 2017-06-01T10:12:35Z 2010 JAN 2010 article Marti, Miguel, P. Trotonda, M., Angeles Tormo-Mas, M., Vergara-Irigaray, Marta, Cheung, Ambrose L., Lasa, Inigo, Penades, J.R. (2010). Extracellular proteases inhibit protein-dependent biofilm formation in Staphylococcus aureus. Microbes and Infection, 12(1), 55-64. 1286-4579 http://hdl.handle.net/20.500.11939/5574 10.1016/j.micinf.2009.10.005 en openAccess Impreso |
| spellingShingle | Marti, Miguel Trotonda, Maria P. Tormo-Mas, María A. Vergara-Irigaray, Marta Cheung, Ambrose L. Lasa, Inigo Penadés, José R. Extracellular proteases inhibit protein-dependent biofilm formation in Staphylococcus aureus |
| title | Extracellular proteases inhibit protein-dependent biofilm formation in Staphylococcus aureus |
| title_full | Extracellular proteases inhibit protein-dependent biofilm formation in Staphylococcus aureus |
| title_fullStr | Extracellular proteases inhibit protein-dependent biofilm formation in Staphylococcus aureus |
| title_full_unstemmed | Extracellular proteases inhibit protein-dependent biofilm formation in Staphylococcus aureus |
| title_short | Extracellular proteases inhibit protein-dependent biofilm formation in Staphylococcus aureus |
| title_sort | extracellular proteases inhibit protein dependent biofilm formation in staphylococcus aureus |
| url | http://hdl.handle.net/20.500.11939/5574 |
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