Morphometric changes in goat sperm heads induced by cryopreservation
Two experiments were designed to evaluate the effect of cryopreservation on morphometric characteristics of the goat sperm head. To address this question, we evaluated the size of the sperm head in fresh control cells, post-cooling cells after equilibration with the glycerol preservation solution, a...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
|
| Acceso en línea: | http://hdl.handle.net/20.500.11939/5567 |
| _version_ | 1855032299174756352 |
|---|---|
| author | Marco-Jiménez, Francisco Viudes-De-Castro, María P. Balasch, Sebastià Mocé, Eva Silvestre, Miguel A. Gómez, Ernesto A. Vicente, José Salvador |
| author_browse | Balasch, Sebastià Gómez, Ernesto A. Marco-Jiménez, Francisco Mocé, Eva Silvestre, Miguel A. Vicente, José Salvador Viudes-De-Castro, María P. |
| author_facet | Marco-Jiménez, Francisco Viudes-De-Castro, María P. Balasch, Sebastià Mocé, Eva Silvestre, Miguel A. Gómez, Ernesto A. Vicente, José Salvador |
| author_sort | Marco-Jiménez, Francisco |
| collection | ReDivia |
| description | Two experiments were designed to evaluate the effect of cryopreservation on morphometric characteristics of the goat sperm head. To address this question, we evaluated the size of the sperm head in fresh control cells, post-cooling cells after equilibration with the glycerol preservation solution, and post-thawing cells. Assessment was by automated morphometric sperm head analysis (ASMA) using phase-contrast microscopy without staining. In the first experiment, ASMA was performed on heterospermic pooled samples (fresh, post-cooling after equilibration with the glycerol preservation solution and post-thawing): length, width, area and perimeter were measured. In the second experiment, sperm viability was assessed by Hoechst staining and head morphometry was carried out its before, simultaneously during the cryopreservation process, and the head size was identified for both live and dead spermatozoa. The data were analysed by principal component analysis (PCA). The purpose of PCA is to derive a small number of linear combinations (principal components) from a set of variables (length, width, area and perimeter), that retain as much of the information in the original variables as possible. The main findings that have emerged from this study are that (i) a simple procedure has been developed for measuring spermatozoa heads without staining, which minimises the possibility that sperm head dimensions were influenced by procedural artefacts: (ii) the dimensions of goat sperm heads after cryopreservation in skimmed milk-glucose medium were smaller than in fresh sperm, but this was due to the equilibration phase with the cryoprotectant and not to the cryopreservation process itself, and (iii) dead spermatozoa showed smaller heads than live sperm, consequent upon the loss of membrane function. No differences were observed between post-cooling cells after equilibration with the glycerol preservation solution and post-thawing spermatozoa and only minor osmotic differences between them and fresh sperm were observed. (c) 2006 Elsevier Inc. All rights reserved. |
| format | article |
| id | ReDivia5567 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia55672025-04-25T14:43:19Z Morphometric changes in goat sperm heads induced by cryopreservation Marco-Jiménez, Francisco Viudes-De-Castro, María P. Balasch, Sebastià Mocé, Eva Silvestre, Miguel A. Gómez, Ernesto A. Vicente, José Salvador Two experiments were designed to evaluate the effect of cryopreservation on morphometric characteristics of the goat sperm head. To address this question, we evaluated the size of the sperm head in fresh control cells, post-cooling cells after equilibration with the glycerol preservation solution, and post-thawing cells. Assessment was by automated morphometric sperm head analysis (ASMA) using phase-contrast microscopy without staining. In the first experiment, ASMA was performed on heterospermic pooled samples (fresh, post-cooling after equilibration with the glycerol preservation solution and post-thawing): length, width, area and perimeter were measured. In the second experiment, sperm viability was assessed by Hoechst staining and head morphometry was carried out its before, simultaneously during the cryopreservation process, and the head size was identified for both live and dead spermatozoa. The data were analysed by principal component analysis (PCA). The purpose of PCA is to derive a small number of linear combinations (principal components) from a set of variables (length, width, area and perimeter), that retain as much of the information in the original variables as possible. The main findings that have emerged from this study are that (i) a simple procedure has been developed for measuring spermatozoa heads without staining, which minimises the possibility that sperm head dimensions were influenced by procedural artefacts: (ii) the dimensions of goat sperm heads after cryopreservation in skimmed milk-glucose medium were smaller than in fresh sperm, but this was due to the equilibration phase with the cryoprotectant and not to the cryopreservation process itself, and (iii) dead spermatozoa showed smaller heads than live sperm, consequent upon the loss of membrane function. No differences were observed between post-cooling cells after equilibration with the glycerol preservation solution and post-thawing spermatozoa and only minor osmotic differences between them and fresh sperm were observed. (c) 2006 Elsevier Inc. All rights reserved. 2017-06-01T10:12:34Z 2017-06-01T10:12:34Z 2006 APR 2006 article Marco-Jimenez, F., Viudes-de-Castro, M.P., Balasch, S., Moce, E., Silvestre, M.A., Gomez, E.A., Vicente, J.S. (2006). Morphometric changes in goat sperm heads induced by cryopreservation. Cryobiology, 52(2), 295-304. 0011-2240 http://hdl.handle.net/20.500.11939/5567 10.1016/j.cryobiol.2006.01.002 en openAccess Impreso |
| spellingShingle | Marco-Jiménez, Francisco Viudes-De-Castro, María P. Balasch, Sebastià Mocé, Eva Silvestre, Miguel A. Gómez, Ernesto A. Vicente, José Salvador Morphometric changes in goat sperm heads induced by cryopreservation |
| title | Morphometric changes in goat sperm heads induced by cryopreservation |
| title_full | Morphometric changes in goat sperm heads induced by cryopreservation |
| title_fullStr | Morphometric changes in goat sperm heads induced by cryopreservation |
| title_full_unstemmed | Morphometric changes in goat sperm heads induced by cryopreservation |
| title_short | Morphometric changes in goat sperm heads induced by cryopreservation |
| title_sort | morphometric changes in goat sperm heads induced by cryopreservation |
| url | http://hdl.handle.net/20.500.11939/5567 |
| work_keys_str_mv | AT marcojimenezfrancisco morphometricchangesingoatspermheadsinducedbycryopreservation AT viudesdecastromariap morphometricchangesingoatspermheadsinducedbycryopreservation AT balaschsebastia morphometricchangesingoatspermheadsinducedbycryopreservation AT moceeva morphometricchangesingoatspermheadsinducedbycryopreservation AT silvestremiguela morphometricchangesingoatspermheadsinducedbycryopreservation AT gomezernestoa morphometricchangesingoatspermheadsinducedbycryopreservation AT vicentejosesalvador morphometricchangesingoatspermheadsinducedbycryopreservation |